Medical Genetics
High MMP-1, MMP-2, and MMP-9 protein levels in osteoarthritis
Authors: G.Q. Zeng, A.B. Chen, W. Li, J.H. Song and C.Y. Gao
Our study examined the relationship between the expression of matrix metalloproteinases (MMP)-1, MMP-2, and MMP-9 proteins and the pathogenesis of osteoarthritis (OA). We employed rigorous inclusion and exclusion criteria in computer-based bibliographic databases to extract published studies relevant to this investigation. The.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.46
- DOI:
- 10.4238/2015.November.18.46
High proportion of mannosidosis and fucosidosis among lysosomal storage diseases in Cuba
Authors: C. MenÃ?©ndez-Sainz, A. GonzÃ?¡lez-Quevedo, S. GonzÃ?¡lez-GarcÃ?Âa, M. PeÃ?±a-SÃ?¡nchez and R. Giugliani
Although lysosomal storage disorders (LSDs) are considered individually rare, as a group they present a non-negligible frequency. Few studies have been made of populational occurrence of LSDs; they have been conducted predominantly on Caucasian populations. We studied the occurrence of LSDs in Cuba. Data from individuals who had been referred to the Insti.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.9
- DOI:
- 10.4238/2012.August.13.9
Meta-analysis demonstrates that the NAD(P)H: quinone oxidoreductase 1 (NQO1) gene 609 C>T polymorphism is associated with increased gastric cancer risk in Asians
Authors: Y. Zhang, Z.T. Wang and J. Zhong
The association between the NAD(P)H: quinone oxidoreductase 1 (NQO1) gene C609T polymorphism and gastric cancer has been widely evaluated, yet with conflicting results. Data were available from seven study populations involving 2600 subjects. Overall, comparison of alleles 609T and 609C indicated a significantly increased risk (46%) for gastric cancer (95.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.6
- DOI:
- 10.4238/2012.August.13.6
Screening for genes that are differentially-expressed between gastric cancer cells and gastric tumor sphere cells using the gene chip technique
Authors: W.H. Shi, Y.B. Yu, Z.-Y. Li, X.-C. Guo, Z.-L. Wei and Z.-H. Zheng
The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both sa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.54
- DOI:
- 10.4238/2015.November.18.54
Molecular cloning and expression of drought-induced protein 3 (DIP3) encoding a class III chitinase in upland rice
Authors: X.L. Guo, L.R. Bai, C.Q. Su, L.R. Shi and D.W. Wang
A drought-induced gene, DIP3, encoding a chitinase III protein was isolated from the roots of upland rice by reverse transcription-polymerase chain reaction (RT-PCR). Sequence analysis demonstrated that the cDNA and deduced protein showed high identity to Oryza sativa class III chitinase. The deduced protein contained a signal peptide sequence in the N-te.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.19.5
- DOI:
- 10.4238/2013.December.19.5
Cloning and characterization of a �²-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae)
Authors: Y. Wu, H.D. Zou, H. Cheng, C.Y. Zhao, L.F. Sun, S.Z. Su, S.P. Li and Y.P. Yuan
Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a prote.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.4
- DOI:
- 10.4238/2012.August.13.4
Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor
Authors: J.W. Wang, W.W. Cheng, T. Xu and Z.Y. Yang
We investigated the effect of propofol on the proliferation and viability of rat embryonic neural stem cells (rENSCs) and the potential mechanisms involved. rENSCs were isolated and cultured in vitro and treated with 1, 10, or 50 μM propofol, while the control group was treated with 0.1 μM dimethyl sulfoxide. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.57
- DOI:
- 10.4238/2015.November.18.57
MLH1 and XRCC1 polymorphisms in Mexican patients with colorectal cancer
Authors: R. MuÃ?±iz-Mendoza, M.L. Ayala-Madrigal, M. Partida-PÃ?©rez, J. Peregrina-Sandoval, E. Leal-Ugarte, N. MacÃ?Âas-GÃ?³mez, V. Peralta-Leal, J.P. Meza-Espinoza, J.M. Moreno-Ortiz, R. RamÃ?Ârez-RamÃ?Ârez, S. SuÃ?¡rez-Villanueva and M. GutiÃ?©rrez-Angulo
DNA repair proteins maintain DNA integrity; polymorphisms in genes coding for these proteins can increase susceptibility to colorectal cancer (CRC) development. We analyzed a possible association of MLH1 -93G>A and 655A>G and XRCC1 Arg194Trp and Arg399Gln polymorphisms with CRC in Mexican patients. Genomic DNA samples were obtained from peripheral b.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.27.6
- DOI:
- 10.4238/2012.June.27.6
Expression profile of the GA733 gene family in colorectal cancer: correlation with clinicopathological parameters
Authors: I. Mamaloudis, D. Zacharoulis, M. Samara, G. Papadopoulos, S. Samara, G. Koukoulis, C. Chatzitheofilou and P. Kollia
GA733-1/-2/-3 genes have been detected in various types of cancer, although their role has not been fully clarified. GA733-2 and GA733-1 have been correlated with lymph node metastases in laryngeal cancer and liver metastases, respectively. Only a few studies have elucidated the mechanisms regulating GA73.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.42
- DOI:
- 10.4238/2015.November.18.42
Effect of ABCE1-silencing gene, transfected by electrotransfer, on the proliferation, invasion, and migration of human thyroid carcinoma SW579 cells
Authors: X. Qu and L. Zhang
We investigated the effect of an ABCE1-silencing gene on the proliferation, invasion, and migration of human thyroid carcinoma SW579 cells. We designed and synthesized targeted ABCE1-siRNA sequences and a negative control sequence (NC-siRNA), and transfected them into human thyroid cancer SW579 cells by electrotransfer to obta.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.32
- DOI:
- 10.4238/2015.November.18.32