Cytogenomics
Association between a microRNA-214 binding site polymorphism in the methylenetetrahydrofolate reductase gene and esophageal squamous cell carcinoma
Authors: G.R. Shen, W.Z. Li, Y.C. Liu, X.P. Li and H.Y. Yuan
MicroRNAs (miRNAs) are key regulators of gene expression and play an important role in the development and progression of various diseases including esophageal squamous cell carcinoma (ESCC). In this study, we determined whether a polymorphism at the miR-214 binding site in the 3'-untranslated region (3'-UTR) of the methylenet.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028158
- DOI:
- 10.4238/gmr.15028158
Mechanisms of cytotoxicity induced by the anesthetic isoflurane: the role of inositol 1,4,5-trisphosphate receptors
Authors: W.-H. Zhai, J. Zhao, S.-P. Huo, X.-G. Chen, Y.-D. Li, Z.-L. Zhang, L.-L. Yu, S. Song and Q.-J. Wang
Isoflurane can induce widespread cytotoxicity. We hypothesized that isoflurane induces apoptosis partly by causing excessive calcium release from the endoplasmic reticulum (ER) via direct activation of inositol 1,4,5-trisphosphate receptors (IP3 R). Rat pheochromocytoma cells cultured for seven days with nerve growth factor were divided into four groups: .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.1
- DOI:
- 10.4238/2015.June.26.1
Cytogenetic and protein quantification in polyploid somatic tissue of Myrmeleon uniformis (Neuroptera, Myrmeleontidae)
Authors: C.A. Pacheco,T.L. da Silva and M.K.H. Kobayashi
Myrmeleon unifomis Malpighian tubules cells were analyzed under two aspects: chromatin structure and total protein quantification in the three larval phases (L1, L2 and L3) and in adults. This insect Malpighian tubules present polyploid tissue resultant from endoreduplication, which probably begins in the first larval phases. The Malpighian tubule proxima.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.1
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.1
Genetic diversity analysis of Capsicum spp germplasm bank accessions based on �±/�²-esterase polymorphism
Authors: E.R. Monteiro, A.R. Bronzato, G.R. Orasmo, A.C.A. Lopes, R.L.F. Gomes, C.A. Mangolin and M.F.P.S. Machado
Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piauí in northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.12.2
- DOI:
- 10.4238/2013.April.12.2
Evaluation of genotoxic and cytotoxic effects of antileishmanial extract from Julocroton triqueter (Euphorbiaceae)
Authors: V.R. Moreira, A.C.S. Dias, A.R.P. Martins, M.I.S. Lima, I.C.P.B. Serra, M.N.S. Ribeiro, F.R.F. Nascimento and S.R.F. Pereira
Julocroton triqueter extracts have antileishmanial activity; however, the effect on genetic stability has not been studied. We evaluated genotoxic and cell death induction potential (in vitro and in vivo) of J. triqueter var. triqueter hydroalcoholic extracts, as well as their antigenotoxic potential in vivo. The in vitro genotoxic studies were performed .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.8
- DOI:
- 10.4238/2013.April.10.8
Genetic diversity analysis of the Uruguayan Creole cattle breed using microsatellites and mtDNA markers
Authors: E. Armstrong, A. Iriarte, A.M. MartÃ?Ânez, M. Feijoo, J.L. Vega-Pla, J.V. Delgado and A. Postiglioni
The Uruguayan Creole cattle population (N = 600) is located in a native habitat in south-east Uruguay. We analyzed its genetic diversity and compared it to other populations of American Creole cattle. A random sample of 64 animals was genotyped for a set of 17 microsatellite loci, and the D-loop hyper-variable region of mtDNA was sequenced for 28 calves o.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.7
- DOI:
- 10.4238/2013.April.10.7
Regulating effects of insertion direction of matrix attachment regions on transgenic expression in stably transformed Chinese hamster ovary cells
Authors: F. Wang, J.H. Zhang, T.Y. Wang, W.H. Dong, X.J. Yang, X.Y. Wang,L. Wang, R. Yang, Q. Li and C.P. Zhao
We investigated the effects of different directions of insertion of matrix attachment region (MAR) sequences on transgenic expression in stably transformed Chinese hamster ovary (CHO) cells. The MAR sequences were inserted in forward or reverse directions into the expression vectors, and transfected into CHO cells. The expression of the chloramphenicol ac.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.12
- DOI:
- 10.4238/2015.June.26.12
Genetic analysis of agronomic traits associated with plant architecture by QTL mapping in maize
Authors: Z.P. Zheng and X.H. Liu
Maize (Zea mays L.) is one of the most important cereal crops worldwide, and increasing the grain yield and biomass has been among the most important goals of maize production. The plant architecture can determine the grain yield and biomass to some extent; however, the genetic basis of the link between the plant architecture and grain yield/biomass is un.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.3
- DOI:
- 10.4238/2013.April.17.3
Cassava interspecific hybrids with increased protein content and improved amino acid profiles
Authors: P.T.C. Gomes and N.M.A. Nassar
Cassava (Manihot esculenta) is a principal food for large populations of poor people in the tropics and subtropics. Its edible roots are poor in protein and lack several essential amino acids. Interspecific hybrids may acquire high protein characteristics from wild species. We analyzed 19 hybrids of M. esculenta with its wild relative, M. oligantha, for c.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.12.8
- DOI:
- 10.4238/2013.April.12.8
Genetic diversity in wild Dipsacus chinensis populations from China based on ISSR markers
Authors: D.-X. Chen, L.-Y. Li, X. Zhang, Y. Wang and Z. Zhang
Wild Dipsacus chinensis plants in China have become endangered owing to over-harvesting and habitat fragmentation. We examined the genetic diversity and genetic structure of 90 individuals from three populations using inter-simple sequence repeat markers and found that 106 of 173 bands amplified by 22 informative and reliable primers were polymorphic. The.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.12.7
- DOI:
- 10.4238/2013.April.12.7