Cytogenomics
Development of highly polymorphic EST-SSR markers and segregation in F1 hybrid population of Vitis vinifera L.
Authors: E. Kayesh, Y.Y. Zhang, G.S. Liu, N. Bilkish, X. Sun, X.P. Leng and J.G. Fang
The objectives of this investigation were to develop and validate the expressed sequence tag (EST)-simple sequence repeat (SSR) markers from large EST sequences, and to study the segregation and distribution of SSRs within two grapevine parental lines. In total, 94 F1 lines crossed between “Early Rose” and “Red Globe” were studied... Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.5
- DOI:
- 10.4238/2013.September.23.5
Cytogenetic toxicity and gonadal effects of 17 �±-methyltestosterone in Astyanax bimaculatus (Characidae) and Oreochromis niloticus (Cichlidae)
Authors: C.L.G. Rivero-Wendt, A.L. Miranda-Vilela, M.F.N. Ferreira, A.M. Borges and C.K. Grisolia
The synthetic hormone, 17-α-methyltestosterone (MT), is used in fish hatcheries to induce male monosex. Androgenic effects on various fish species have been reported; however, few studies have assessed possible genotoxic effects, although there are concerns about such effects in target and non-target species. We evaluated genotoxic and gonadal effec.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.4
- DOI:
- 10.4238/2013.September.23.4
Effect of glycerol on GHR and IGF-1 gene expression in breast muscle and on growth of Japanese meat quails
Authors: S.C.C. Silva, E. Gasparino, D.M. Voltolini, A.V. Pires, F. Tanamati, A.L.S. Garcia and D.P. de Oliveira
We evaluated messenger RNA (mRNA) expression of the growth-hormone (GHR) and insulin-like growth factor (IGF-1) genes in 28-day-old Japanese meat quails fed diets containing 0, 8, or 12% dietary glycerol in substitution of corn. Total RNA was extracted from the breast muscle and the DNA was amplified with specific primers using real-time PCR. Feed convers.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.3
- DOI:
- 10.4238/2013.September.23.3
Fine mapping and characterization of a novel dwarf and narrow-leaf mutant dnl1 in rice
Authors: X.J. Wei, S.Q. Tang, G.N. Shao, M.L. Chen, Y.C. Hu and P.S. Hu
Plant height is one of the most important agronomic traits of rice (Oryza sativa). Dwarf mutants are ideal materials for research on the mechanisms of regulation of rice plant height. We examined a new dwarf and narrow-leaf mutant dnl1. Phenotypic analysis showed that the dnl1 mutant has a thinner culm and more tillers, but the number of grains per panicl.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.2
- DOI:
- 10.4238/2013.September.23.2
Expression of a GDP-L-galactose phosphorylase-like gene in a Chinese wild Vitis species induces responses to Erysiphe necator and defense signaling molecules
Authors: H.M. Hou, H.E. Li, M. Gao, H. Wang, C. Jiao and X.P. Wang
Using rapid amplification of cDNA ends, a full-length cDNA sequence of a GDP-L-galactose phosphorylase-like gene was isolated from leaves infected by Erysiphe necator in the Chinese wild (Vitis pseudoreticulata) clone, ‘Baihe-35-1’, an E. necator-resistant genotype. The full-length cDNA, designated as VpVTC, comprised 1943 bp and putatively en.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.1
- DOI:
- 10.4238/2013.September.23.1
Association of cytokine gene polymorphisms with susceptibility to invasive candidiasis
Authors: R.T. Sun, W.J. Tian, X.W. Xing, S.H. Gao and S.B. Wang
The aim of this study was to investigate the role of cytokine genes in the susceptibility to Candida infection. A total of 275 consecutive patients diagnosed with Candida infection were selected between May 2010 and May 2011, along with 305 uninfected controls. Genotyping of the IL-1β gene polymorphisms (IL1β) rs1143634, IL1β rs16944, IL8 r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.29
- DOI:
- 10.4238/2015.June.18.29
Polymorphisms of the myostatin gene (MSTN) and its relationship with growth traits in goat breeds
Authors: Z.J. Zhang, Y.H. Ling, L.J. Wang, Y.F. Hang, X.F. Guo, Y.H. Zhang, J.P. Dingand X.R. Zhang
Mutations in the myostatin (MSTN) gene can inactivate its expression and result in a non-functional protein, which leads to dramatic muscularity and a “double-muscling” phenomenon in many species. Using gene sequencing and polymerase chain reaction-single-strand conformation polymorphism methods, polymorphisms of the MSTN gene were investigate.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.12
- DOI:
- 10.4238/2013.April.2.12
cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)
Authors: Q. Zou, P. Wei, Q. Xu, H.Z. Zheng, B. Tang and S.G. Wang
The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and funct.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.7
- DOI:
- 10.4238/2013.April.2.7
Genetic variability in Blanco Orejinegro breed cattle populations in Colombia
Authors: R. MartÃ?Ânez, A.P. LlinÃ?¡s and J.F. M.-Rocha
We analyzed population structure and genetic diversity in Blanco Orejinegro Creole cattle with 12 microsatellite markers, genotyping 138 individuals belonging to 7 commercial and 3 conservation herds. These markers showed a high level of polymorphism; 171 alleles were identified. The mean number of alleles per locus was 5.63 (3.82-6.58). The total number .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.4
- DOI:
- 10.4238/2013.April.10.4
The epimer of kaurenoic acid from Croton antisyphiliticus is cytotoxic toward B-16 and HeLa tumor cells through apoptosis induction
Authors: V.C. Fernandes, S.I.V. Pereira, J. Coppede, J.S. Martins, W.F. Rizo, R.O. Beleboni, M. Marins, P.S. Pereira, A.M.S. Pereira and A.L. Fachin
Cancer has become the leading cause of death in developing countries due to increased life expectancy of the population and changes in lifestyle. Studies on active principles of plant have motivated researchers to develop new antitumor agents that are specific and effective for treatment of neoplasms. Kaurane diterpenes are considered important compounds .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.16
- DOI:
- 10.4238/2013.April.2.16