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Cloning of flanking sequence in transgenic plants by restriction site-anchored single-primer polymerase chain reaction
Authors: J. Ma, N.N. Wang, S. Ren, Y.P. Fu, S. Lu, Y.P. Wang and P.W. Wang
Determining the insertion position of an exogenous gene in the target plant genome is one of the main issues in the transgenic plant field. This study introduced a simple, rapid, and accurate method to clone the flanking sequences of the transgenic bar gene as the anchoring gene in the transgenic maize genome using single-primer polymerase chain reaction .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.18
- DOI:
- 10.4238/2014.December.12.18
Weak cation magnetic separation technology and MALDI-TOF-MS in screening serum protein markers in primary type I osteoporosis
Authors: X.L. Shi, C.W. Li, B.C. Liang, K.H. He and X.Y. Li
We investigated weak cation magnetic separation technology and matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) in screening serum protein markers of primary type I osteoporosis. We selected 16 postmenopausal women with osteoporosis and nine postmenopausal women as controls to find a .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.4
- DOI:
- 10.4238/2015.November.30.4
Clinical and cytogenetic results of a series of amniocentesis cases from Northeast China: a report of 2500 cases
Authors: N. An, L.L. Li, R.X. Wang, L.L. Li, J.M. Yue and R.Z. Liu
The aims of this study were to demonstrate the clinical and cytogenetic results of amniocentesis (AS) cases in Northeast China, to compare the incidence of different kinds of chromosomal abnormalities, and to study the association between the detection rate of chromosomal abnormalities and different indications for prenatal di.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.18
- DOI:
- 10.4238/2015.December.1.18
Cuticular signature in the development of Polistes versicolor
Authors: J.H. dos S. Brito, T.S. Montagna, F.S. Maia, W.F. Antonialli-Junior and C.A.L. Cardoso
Wasps belong to societies that are highly complex and diverse, especially considering social organization and parental care. They use chemicals in their daily communication, and act incisively in recognition of mates and non-nest mates, and can even identify individuals of different castes. In this study, cuticle composition w.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.19
- DOI:
- 10.4238/2015.October.16.19
`Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients
Authors: C.L. Wei1, J.L. Cheng, W.C. Yang, L.Y. Li, H.C. Cheng and J.J. Fu1
Y chromosomal microdeletions at the azoospermia factor locus and chromosome abnormalities have been implicated as the major causes of idiopathic male infertility. A marker chromosome is a structurally abnormal chromosome in which no part can be identified by cytogenetics. In this study, to identify the origin of the marker chromosomes and to perform a gen.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.11
- DOI:
- 10.4238/2015.November.18.11
Protein expression and characterization of SEP3 from Arabidopsis thaliana
Authors: Q. Shi, J. Zhou, P. Wang, X. Lin and Y. Xu
SEPALLATA (SEP) MADS-box genes play crucial roles in the regulation of floral growth and development. They are required for the specification of sepals, petals, stamens, and carpels as well as for floral determinacy. SEPs perform their functions through the formation of homo- or hetero-polymers, which are the molecular basis o.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.16.20
- DOI:
- 10.4238/2015.October.16.20
Cloning and characterization of a benzoic acid/salicylic acid carboxyl methyltransferase gene involved in floral scent production from lily (Lilium �Yelloween�)
Authors: H. Wang, M. Sun, L.L. Li, X.H. Xie and Q.X. Zhang
In lily flowers, the volatile ester methyl benzoate is one of the major and abundant floral scent compounds; however, knowledge regarding the biosynthesis of methyl benzoate remains unknown for Lilium. In this study, we isolated a benzoic acid/salicylic acid carboxyl methyltransferase (BSMT) gene, LiBSMT, from petals of Lilium ‘Yelloween’. The.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.14
- DOI:
- 10.4238/2015.November.18.14
Proposed method for dimensionality reduction based on framework in gene expression domain
Authors: D.C. Macedo, E.C.M. Ishikawa, C.B. Santos, S.N. Matos, H.B. Borges and A.C. Francisco
The excessive use of attributes may affect the search for patterns and extraction of useful knowledge, because they harm the learning performance of algorithms in both speed and success rate. The use of dimensionality reduction methods is therefore an important alternative; however, these methods do not deal with the reduction of attributes in a specific .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.21
- DOI:
- 10.4238/2014.December.12.21
Optimization of candidate proteins for serological screening of Chlamydia trachomatis infection
Authors: X.B. Gao, M. Xiao, J. Wang, Y.J. Liu, Q.Z. Liu and M.L. Qi
The aim of this study was to optimize candidate antigen proteins for serological screening of Chlamydia trachomatis infection. C. trachomatis positive serum and swabs of genital secretions were collected from 50 patients in the Tianjin Medical University General Hospital, as well as from 30 patients negative for C. trachomatis.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.12
- DOI:
- 10.4238/2015.October.9.12
Spermatogenesis and nucleolar behavior in Triatoma vandae and Triatoma williami (Hemiptera, Triatominae)
Authors: N.P. Pereira, K.C.C. Alevi, P.P. Mendon�§a and M.T.V. Azeredo-Oliveira
This study describes spermatogenesis in Triatoma vandae and the nucleolar behavior of T. vandae and Triatoma williami, with a cytotaxonomic focus. Analysis of mitotic and meiotic metaphases of T.vandae confirms the species karyotype. T. vandae presents some characteristics during meiosis that differentiate it from T. williami,.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.2
- DOI:
- 10.4238/2015.October.9.2