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New genomic resources for the honey bee(Apis mellifera L.): development of a deep-coverage BAC library and a preliminary STC database
Authors: J.P. Tomkins, M. Luo, G.C. Fang1, D. Main1, J.L. Goicoechea,M. Atkins, D.A. Frisch, R.E. Page, E. Guzm�¡n-Novoa5, Y. Yu,G. Hunt6 and R.A. Wing
We have constructed a bacterial artificial chromosome (BAC) library for a European honey bee strain using the cloning enzyme HindIII in order to develop resources for structural genomics research. The library contains 36,864 clones (ninety-six 384-well plates). A random sampling of 247 clones indicated an average insert size o.. Read More»
- Genet. Mol. Res. 1(4):
(Co)variance components, genetic parameters and annual trends for calf weights in a Brahman herd kept on floodable savanna
Authors: Dieter Plasse1, Omar Verde, Jes�ºs Arango, Luis Camaripano, Hugo Fossi, Rafael Romero, Carlos Rodriguez M. and Jos�© L. Rumbos
(Co)variance components and genetic parameters were estimated for body weights of an elite Brahman herd under a designed, supervised management and genetic program, including strategic artificial insemination (AI). Restricted maximum likelihood methods were used with a univariate animal model for birth weight (BW) and a bivari.. Read More»
- Genet. Mol. Res. 1(4):
Greater genetic variability in Argentine Creole than in Thoroughbred horses based on serum protein polymorphisms
Authors: S. DÃ?Âaz, F.N. Dulout and P. Peral-GarcÃ?Âa
Genetic polymorphism was analyzed for five blood proteins: albumin - Al, esterase - Es, α1B-glycoprotein - Xk, transferrin - Tf and hemoglobin - Hb in 200 Thoroughbred (TB) and 124 Argentine Creole (AC) horses. Of the five systems examined, Tf and Hb were not in Hardy-Weinberg equilibrium in either breed and Es was not i.. Read More»
- Genet. Mol. Res. 1(3):
Frequencies of GSTM1, GSTT1, and GSTP1 polymorphisms in a Brazilian population
Authors: Ana Rossini, Davy C.M. Rapozo, LÃ?Âdia M.F. Amorim, Jacyara M.B. Macedo1, Raquel Medina, JosÃ?© F.N. Neto, ClÃ?¡udia V.M. Gallo and Luis F.R. Pinto
The glutathione S-transferase (GST) family of enzymes has a vital role in phase II of biotransformation of environmental carcinogens, pollutants, drugs and other xenobiotics. GSTs are polymorphic, with the type and frequency of polymorphism being ethnic dependent. Polymorphisms in GST genes have been shown to be associated wit.. Read More»
- Genet. Mol. Res. 1(3):
Female remating, sperm competition and sexual selection in Drosophila
Authors: Shree Ram Singh, Bashisth N. Singh and Hugo F. Hoenigsberg
Female remating is fundamental to evolutionary biology as it determines the pattern of sexual selection and sexual conflict. Remating in females is an important component of Drosophila mating systems because it affects sperm usage patterns and sexual selection. Remating is common in females of many species of Drosophila in bot.. Read More»
- Genet. Mol. Res. 1(3):
Varroa-tolerant Italian honey bees introduced from Brazil were not more efficient in defending themselves against the mite Varroa destructor than Carniolan bees in Germany
Authors: M.H. Corr�ªa-Marques, D. De Jong, P. Rosenkranz andL.S. Gon�§alves
In Europe and North America honey bees cannot be kept without chemical treatments against Varroa destructor. Nevertheless, in Brazil an isolated population of Italian honey bees has been kept on an island since 1984 without treatment against this mite. The infestation rates in these colonies have decreased over the years. We l.. Read More»
- Genet. Mol. Res. 1(2):
Cell nucleus activity during post-embryonic development of Apis mellifera L. (Hymenoptera:Apidae). Intranuclear acid phosphatase
Authors: Carminda da Cruz Landim, Rejane Daniele Reginato, Regina Lucia Morelli Silva de Moraes and Vagner Melo Cavalcante
We report nuclear acid phosphatase activity in the somatic (intra-ovariolar and stromatic) and germ cells of differentiating honey bee worker ovaries, as well as in the midgut cells of metamorphosing bees. There was heterogeneity in the intensity and distribution of electron dense deposits of lead phosphate, indicative of acid.. Read More»
- Genet. Mol. Res. 1(2):
Identification and characterization of polymorphisms at the HSA �±1-acid glycoprotein (ORM*) gene locus in Caucasians
Authors: Catherine M. Owczarek, Aleksander L. Owczarek andPhilip G. Board
Human α1-acid glycoprotein (AGP) or orosomucoid (ORM) is a major acute phase protein that is thought to play a crucial role in maintaining homeostasis. Human AGP is the product of a cluster of at least two adjacent genes located on HSA chromosome 9. Using a range of restriction endonucleases we have investigated DNA vari.. Read More»
- Genet. Mol. Res. 1(1):
Estimates of heritability in a blanched asparagus population
Authors: Vanina Cravero, Enrique Cointry, Ileana Gatti and Fernando L�³pez Anido
To estimate the heritability values of characters frequently used as selective criteria, 32 half-sib families obtained from selected plants of three populations of the asparagus variety Argenteüil were evaluated in a randomized complete block design. The following characters were measured: days to emergence of the first s.. Read More»
- Genet. Mol. Res. 1(1):
Rapid identification of single nucleotide polymorphisms by fluorescence-based capillary electrophoresis
Authors: Montse Bernat, Esther Titos and Joan Cl� ria
We describe the application of two different fluorescence- based techniques (ddNTP primer extension and single-strand conformation polymorphism (SSCP)) to the detection of single nucleotide polymorphisms (SNPs) by capillary electrophoresis. The ddNTP primer extension technique is based on the extension, in the presence of fluo.. Read More»
- Genet. Mol. Res. 1(1):