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Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs
Authors: Melissa Nunes Miziara, Penny K. Riggs and M. Elisabete J. Amaral
Genomic sequence comparison across species has enabled the elucidation of important coding and regulatory sequences encoded within DNA. Of particular interest are the noncoding regulatory sequences, which influence gene transcriptional and posttranscriptional processes. A phylogenetic footprinting strategy was employed to iden.. Read More»
- Genet. Mol. Res. 3(4):
Easy detection of green fluorescent proteinmulticopy transformants in Penicillium griseoroseum
Authors: Francis J.F. Lopes, Elza F. de Ara�ºjo and Marisa V. de Queiroz
Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the p.. Read More»
- Genet. Mol. Res. 3(4):
Reduction of non-digestible oligosaccharides in soy milk: application of engineered lactic acid bacteria that produce �±-galactosidase
Authors: Jean Guy LeBlanc, Aurelio Silvestroni, Cristelle Connes,Vincent Juillard, Graciela Savoy de Giori, Jean-Christophe Piard and Fernando Sesma
Human consumption of soy-derived products has been limited by the presence of non-digestible oligosaccharides (NDO), such as the a-galactooligosaccharides raffinose and stachyose. Most mammals, including man, lack pancreatic a-galactosidase (a-Gal), which is necessary for the hydrolysis of these sugars. However, such NDO can b.. Read More»
- Genet. Mol. Res. 3(3):
Pro domain peptide of HGCP-Iv cysteine proteinase inhibits nematode cysteine proteinases
Authors: Francine B. Silva, Jo�£o A.N. Batista, Brener M. Marra,Rodrigo R. Fragoso, Ana Carolina S. Monteiro, Edson L.Z. Figueira, and Maria F�¡tima Grossi-de-S�¡
Cysteine proteinases (CPs) are synthesized as zymogens and converted to mature proteinase forms by proteolytic cleavage and release of their pro domain peptides. A cDNA encoding a papain-like CP, called hgcp-Iv, was isolated from a Heterodera glycines J2 cDNA library, expressed and utilized to assess the ability of its propept.. Read More»
- Genet. Mol. Res. 3(3):
The potential of virus-induced gene silencing for speeding up functional characterization of plant genes
Authors: Vagner A. Benedito, Peter B. Visser, Gerco C. Angenent and Frans A. Krens
Virus-induced gene silencing (VIGS) has been shown to be of great potential in plant reverse genetics. Advantages of VIGS over other approaches, such as T-DNA or transposon tagging, include the circumvention of plant transformation, methodological simplicity and robustness, and speedy results. These features make VIGS an attra.. Read More»
- Genet. Mol. Res. 3(3):
Cytogenetics of genetic counseling patients in Pelotas, Rio Grande do Sul, Brazil
Authors: A.C. Duarte1, E. Cunha, J.M. Roth, F.L.S. Ferreira, G.L. Garcias and M.G. Martino-Roth
From 1986 to 2002, we examined the chromosomal composition of 916 patients attended by two genetic counseling services in the city of Pelotas, in the Brazilian State of Rio Grande do Sul, to determine the genetic causes of their disturbances. Patterns of G-banding using trypsin and Giemsa (GTG) and C-banding using barium and G.. Read More»
- Genet. Mol. Res. 3(3):
Knockdown of chimeric glucoce rebrosidase by green fluorescent protein-directed small interfering RNA
Authors: Tessa N. Campbell, Francis Y.M. Choy
Gaucher disease, the most common type of lysosomal storage disorder, is characterized by an inherited deficiency of the membrane-associated hydrolase, glucocerebrosidase. Glucocerebrosidase catalyzes the hydrolysis of glucocerebroside to ceramide and glucose, a crucial step in the recycling of membrane sphingolipids. The exorb.. Read More»
- Genet. Mol. Res. 3(2):
Genetic organization and expression of citrate permease in lactic acid bacteria
Authors: Djamel Drider, Sadjia Bekal and Herve Prevost
Citrate is present in many natural substrates, such as milk, vegetables and fruits, and its metabolism by lactic acid bacteria (LAB) plays an important role in food fermentation. The industrial importance of LAB stems mainly from their ability to convert carbohydrates into lactic acid and, in some species, like Lactococcus lac.. Read More»
- Genet. Mol. Res. 3(2):
A low prevalence of cystic fibrosis in Uruguayans of mainly European descent
Authors: Horacio Cardoso, Beatriz Crispino, Adriana Mimbacas and Manuel Enrique Cardoso
Cystic fibrosis is the most common hereditary disease in populations of European descent, with its prevalence depending on the populations and ethnic groups studied. In contrast to Europe and North America, there is little information about this disease in Latin America. Uruguay currently has a human population of 3,000,000, w.. Read More»
- Genet. Mol. Res. 3(2):
Diagnosis of human visceral leishmaniasis by PCR using blood samples spotted on filter paper
Authors: Eduardo Sergio da Silva, C�©lia Maria Ferreira Gontijo,Raquel da Silva Pacheco and Reginaldo Pe�§anha Brazil
The polymerase chain reaction (PCR) is a simple, rapid procedure that has been adapted for the diagnosis of leishmaniasis. In the present study, 85 blood samples and seven bone marrow aspirates from 85 patients with clinical symptoms suggestive of visceral leishmaniasis from the metropolitan region of Belo Horizonte in the Bra.. Read More»
- Genet. Mol. Res. 3(2):