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Cloning of flanking sequence in transgenic plants by restriction site-anchored single-primer polymerase chain reaction

Author(s): J. Ma, N.N. Wang, S. Ren, Y.P. Fu, S. Lu, Y.P. Wang and P.W. Wang

Determining the insertion position of an exogenous gene in the target plant genome is one of the main issues in the transgenic plant field. This study introduced a simple, rapid, and accurate method to clone the flanking sequences of the transgenic bar gene as the anchoring gene in the transgenic maize genome using single-primer polymerase chain reaction (PCR). This method was based on the distribution of restriction sites in the maize genome and adopted the single-primer PCR method.