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Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep
Authors: M. Yang, Y.J. Yue, T.T. Guo, J.L. Han, J.B. Liu, J. Guo, X.P. Sun, R.L. Feng, Y.Y. Wu, C.F. Wang, L.P. Wang and B.H. Yang
Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.7
- DOI:
- 10.4238/2014.April.8.7
Preventive effect of tert-butylhydroquinone on scrotal heat-induced damage in mouse testes
Authors: Y.S. Li1, Y.G. Piao1, K. Nagaoka2,3, G. Watanabe2,3, K. Taya2,3 and C.M. Li1
To investigate the effect of tert-butylhydroquinone (tBHQ) on scrotal heat-induced damage in mice testes, 8-week-old mice were divided into 6 groups and administered with or without tBHQ through diet (10 mg/g), intraperitoneal injection (100 mg/kg body weight), or intratestis injection (12.5 mg/kg body weight), respectively. After single scrotal heat expo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.5
- DOI:
- 10.4238/2013.November.11.5
Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)
Authors: R.A. Khan1, M. Rashid1, D. Wang2 and Y.L. Zhang1
Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression ve.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.3
- DOI:
- 10.4238/2013.October.10.3
Global comparison of gene expression between subcutaneous and intramuscular adipose tissue of mature Erhualian pig
Authors: W.X. Sun1, H.H. Wang1,2, B.C. Jiang1, Y.Y. Zhao1, Z.R. Xie1, K. Xiong1 and J. Chen1
Adipose tissue plays an important role in energy metabolism and related diseases. The content of intramuscular fat significantly influences the pork quality. In this study, the whole gene expression of dorsal subcutaneous (s.c.) adipose tissue and intramuscular (i.m.) adipose tissue isolated from longissimus dorsi muscle tissue were compared using Affymet.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.3
- DOI:
- 10.4238/2013.October.29.3
Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.
Authors: C.R. Li1,2*, D.D. Liang1,2*, R.F. Xu1,2*, H. Li2,3, Y.P. Zhang2, R.Y. Qin2, L. Li1,2, P.C. Wei2,3 and J.B. Yang2
Low temperature is a major environmental stress in rice cultivating and production. The alternative oxidase 1 (AOX1) gene is potentially important for genetic engineering to increase cold adaptation. However, previous studies related to this effect have mostly focused on the dicot plants Arabidopsis and tobacco, whereas functional research on rice is limi.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.4
- DOI:
- 10.4238/2013.November.11.4
HLA-B35, a common genetic trait, in a familial case of Henoch-Schoenlein purpura and Berger�s disease
Authors: M.C. Pellegrin, L. Matarazzo, E. Neri, M. Pennesi and S. Crovella
Nephritis characterized by IgA mesangial depositions has been described both in Henoch-Schoenlein purpura (HSP) and in Berger’s disease (BD), but common genetic traits are still uncertain. We report here the case of two brothers, the first affected by HSP with persistent nephritis and the second by BD, accidentally disco.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.9
- DOI:
- 10.4238/2014.April.8.9
Study of white matter at the centrum semiovale level with magnetic resonance spectroscopy and diffusion tensor imaging in cerebral small vessel disease
Authors: L.A. Huang, X.Y. Ling, C. Li, S.J. Zhang, G.B. Chi and A.D. Xu
White matter lesion (WML) in magnetic resonance imaging is commonly observed in patients with cerebral small vessel disease (SVD), but the pathological mechanism of WML in SVD is still unclear. We observed the metabolism and microscopic anatomy of white matter in SVD patients. Twelve subjects clinically diagnosed with SVD and .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.8.11
- DOI:
- 10.4238/2014.April.8.11
Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense
Authors: Z. Li, W.Q. Wang, E.F. Zhang and G.F. Qiu
Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, fi ve RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.23.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.23.2
Analysis of GATA1 mutations and leukemogenesis in newborns with Down syndrome
Authors: L.B. Queiroz, B.D. Lima, J.F. Mazzeu, R. Camargo, M.S. C�³rdoba, I.Q. Magalh�£es, C. Martins-de-S�¡ and I. Ferrari
It has been reported that patients with Down syndrome (DS) frequently develop transient myeloproliferative disorder (TMD) and less commonly myeloid leukemia in DS (ML-DS). We examined the pathogenetic relationship of these conditions with somatic mutations of the GATA1 gene in children with both TMD and ML-DS. To determine the incidence of GATA1 mutations.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.1
- DOI:
- 10.4238/2013.October.18.1
Electrophoresis and spectrometric analyses of adaptation-related proteins in thermally stressed Chromobacterium violaceum
Authors: I.B. Cordeiro1, D.P. Castro7, P.P.O. Nogueira7, P.C.S. Angelo6, P.A. Nogueira7, J.F.C. Gon�§alves8, A.M.R.F. Pereira9, J.S. Garcia2,3, G.H.M.F. Souza2,4, M.A.Z. Arruda5, M.N. Eberlin2, S. Astolfi-Filho1, E.V. Andrade1 and J.L. L�³pez-Lozano10
Chromobacterium violaceum is a Gram-negative proteobacteria found in water and soil; it is widely distributed in tropical and subtropical regions, such as the Amazon rainforest. We examined protein expression changes that occur in C. violaceum at different growth temperatures using electrophoresis and mass spectrometry. The total number of spots detected .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.29.1
- DOI:
- 10.4238/2013.October.29.1