Molecular Markers
Identification of Colletotrichum isolates from Capsicum chinense in Amazon
Authors: C.V.S. de Oliveira, K.S. Matos, D.M.C. de Albuquerque, R.E. Hanada and G.F. da Silva
Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colle.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029601
- DOI:
- 10.4238/gmr16029601
Assessment of allelic diversity among drought-resistant cotton genotypes using microsatellite markers
Authors: A. Javaid, F.S. Awan, F.M. Azhar and I.A. Khan
Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (S.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029664
- DOI:
- 10.4238/gmr16029664
Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits
Authors: J.C. Costa, G.G.M. Fracetto, F.J.C. Fracetto, M.V.F. Santos and M.A. Lira Junior
Desmanthus is a genus of forage legumes with high nutritional value, productive potential, and ability to obtain nitrogen in association with diazotrophic bacteria. The use of accurate techniques for genotype identification and characterization is essential for breeding programs. Morphological markers are widely used to know t.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029667
- DOI:
- 10.4238/gmr16029667
Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down stream processes
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu, N. Na and Z. Huiyan
We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from etiolated leaves of wheat crop (Triticum aestivum). mtDNA extraction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applicati.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.27
- DOI:
- 10.4238/2014.December.4.27
Polymorphic microsatellite markers for the rare and endangered cactus Uebelmannia pectinifera (Cactaceae) and its congeneric species
Authors: E.M. Moraes1, F.W. Cidade1, G.A.R. Silva1 and M.C. Machado2
The cactus genus Uebelmannia includes 3 narrow endemic species associated with rocky savanna habitats in eastern South America. Because of their rarity and illegal over-collection, all of these species are endangered. Taxonomic uncertainties resulting from dramatic local variation in morphology within Uebelmannia species preclude effective conservation ef.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.31
- DOI:
- 10.4238/2014.December.4.31
Genetic-molecular characterization of backcross generations for sexual conversion in papaya (Carica papaya L.)
Authors: H.C.C. Ramos1, M.G. Pereira1, T.N.S. Pereira1, G.B.A. Barros1 and G.A. Ferreguetti2
The low number of improved cultivars limits the expansion of the papaya crop, particularly because of the time required for the development of new varieties using classical procedures. Molecular techniques associated with conventional procedures accelerate this process and allow targeted improvements. Thus, we used microsatellite markers to perform geneti.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.32
- DOI:
- 10.4238/2014.December.4.32
Genetic variations in the leptin gene associated with growth and carcass traits in Nellore cattle
Authors: D.B.S. Silva, B.A. Crispim, L.E. Silva, J.A. Oliveira, F. Siqueira, L.O. Seno and A.B. Grisolia
The aim of the study was to detect polymorphisms in the leptin gene and to determine the association of these polymorphisms with growth and carcass traits in Nellore cattle. The single nucleotide polymorphisms (SNPs) -1457 (AJ571671:g.-1457A>G) and A59V (AF536174.1:g. 321C>T), as well as the microsatellite BM1500 (3.9 kb.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.9
- DOI:
- 10.4238/2014.April.16.9
Isolation and characterization of polymorphic microsatellite loci in the swimming crab Portunus trituberculatus (Portunidae) L.P.
Authors: L.P. Ren, Y. Qin, X.C. Li, Y.N. Sun, R.X. Wang
The Swimming crab Portunus trituberculatus (Portunidae) is an important economically food species. To provide molecular markers for P. trituberculatus, we isolated and characterized polymorphic microsatellite markers. We developed a 5'-anchored genomic library of P. trituberculatus DNA, and derived 45 positive clones. We desig.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.19
- DOI:
- 10.4238/2013.November.22.19
Isolation and characterization of microsatellites for the yam Dioscorea cayenensis (Dioscoreaceae) and cross-amplification in D. rotundata
Authors: L.R.G. Silva, M.M. Bajay, M. Monteiro, T.F. Mezette, W.F. Nascimento, M.I. Zucchi, J.B. Pinheiro and E.A. Veasey
Dioscorea cayenensis and Dioscorea rotundata are among the most important yam species for the humid and sub-humid tropics. We isolated nine polymorphic microsatellite markers using a microsatellite-enriched genomic library technique. The nine primer pairs were validated in 22 D. cayenensis accessions, and were tested for trans.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.14.5
- DOI:
- 10.4238/2014.April.14.5
Genetic diversity and structure of natural Dactylis glomerata L. populations revealed by morphological and microsatellite-based (SSR/ISSR) markers
Authors: P. Madesis1, E.M. Abraham2, A. Kalivas, Ã?Â?. Ganopoulos and A. Tsaftaris
Dactylis glomerata L. is an important forage species in the Mediterranean region, and in other regions with a similar climate. Genetic material from 3 locations in north, central, and south Greece was studied, using morphological traits, SSR, and ISSR molecular markers. Morphological analysis revealed differences among the geo.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.8
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.8