Molecular Markers
Thirty-four Musa (Musaceae) expressed sequence tag-derived microsatellite markers transferred to Musella lasiocarpa
Authors: W.J. Li, H. Ma, Z.H. Li, Y.M. Wan, X.X. Liu and C.L. Zhou
We assembled 31,308 publicly available Musa EST sequences into 21,129 unigenes; 4944 of them contained 5416 SSR motifs. In all, 238 unigenes flanking SSRs were randomly selected for primer design and then tested for amplification in Musella lasiocarpa. Seventy-eight primer pairs were found to be transferable to this species, and 49 displayed polymorphism... Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.13
- DOI:
- 10.4238/2012.August.6.13
A new strategy employed for identification of sweet orange cultivars with RAPD markers
Authors: X. Sun, Q. Mu, D. Jiang , C. Wang, X.C. Wang and J.G. Fang
We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.11
- DOI:
- 10.4238/2012.August.6.11
Verification and evaluation of grain QTLs using RILs from TD70 x Kasalath in rice
Authors: Y.D. Zhang, J. Zheng, Z.K. Liang, Y.L. Liang, Z.H. Peng and C.L. Wang
Grain size is an important trait that directly influences the yield of rice. Validation and evaluation of grain genes is important in rice genetic studies and for breeding. In a population of 240 recombinant inbred lines (RILs) derived from a cross between an extra-large grain japonica variety TD70 and a small grain .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.53
- DOI:
- 10.4238/2015.November.18.53
Genetic structure and diversity analysis of the primary gene pool of chickpea using SSR markers
Authors: P. Choudhary, S.M. Khanna, P.K. Jain, C. Bharadwaj, J. Kumar, P.C. Lakhera and R. Srinivasan
Members of the primary gene pool of the chickpea, including 38 accessions of Cicer arietinum, six of C. reticulatum and four of C. echinospermum grown in India were investigated using 100 SSR markers to analyze their genetic structure, diversity and relationships. We found considerable diversity, with a mean of 4.8 alleles per.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.5
- DOI:
- 10.4238/2012.April.10.5
Genetic variability in local Brazilian horse lines using microsatellite markers
Authors: A.C.M. Silva, S.R. Paiva, M.S.M. Albuquerque, A.A. Egito, S.A. Santos, F.C. Lima, S.T. Castro, A.S. Mariante, P.S. Correa and C.M. McManus
Genetic variability at 11 microsatellite markers was analyzed in five naturalized/local Brazilian horse breeds or genetic groups. Blood samples were collected from 328 animals of the breeds Campeira (Santa Catarina State), Lavradeira (Roraima State), Pantaneira (Pantanal Mato-Grossense), Mangalarga Marchador (Minas Gerais Stat.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.4
- DOI:
- 10.4238/2012.April.10.4
An improved strategy based on RAPD markers efficiently identified 95 peach cultivars
Authors: M.L. Yu, W.Y. Wang, R.J. Ma, Z.J. Shen and J.G. Fang
DNA markers have useful applications in cultivar identification. A novel analysis approach called cultivar identification diagram (CID) was developed using DNA markers in the separation of plant individuals. This new strategy is less time- and cost-consuming, has reliable results, and was constructed for fingerprinting. Ten 11.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.7.1
- DOI:
- 10.4238/2012.May.7.1
New microsatellite markers for the abalone Haliotis midae developed by 454 pyrosequencing and in silico analyses
Authors: R. Slabbert J.-A. Hepple C. Rhode A.E. Bester-Van der Merwe R. Roodt-Wilding
Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enrich.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.2
- DOI:
- 10.4238/2012.August.24.2
Monosomic and molecular mapping of adult plant leaf rust resistance genes in the Brazilian wheat cultivar Toropi
Authors: P.R. Da-Silva S.P. Brammer D. Guerra S.C.K. Milach A.L. Barcellos M.I. Baggio
Leaf rust is one of the most destructive diseases affecting wheat worldwide. The most effective way to control it is to use resistant cultivars. Resistance based on slow-rusting adult plant resistance (APR) genes has proven to be the best method for developing cultivars with durable resistance. A source of slow-rusting APR for leaf rust is the Brazilian whea.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.7
- DOI:
- 10.4238/2012.August.24.7
Molecular characterization of wheat germplasm using microsatellite markers
Authors: S. Ijaz and I.A. Khan
We investigated the genetic diversity of 63 wheat genotypes, composed of 48 accessions and 15 varieties, using 56 polymorphic simple sequence repeat primers. One hundred and eighty-six loci were found, with a mean of 131.26 alleles per locus. Cluster analysis based on microsatellite allelic diversity discriminated the accessions and varieties into di.. Read More»
- Genet. Mol. Res. 8(3):
Genetic distances between popcorn populations based on molecular markers and correlations with heterosis estimates made by diallel analysis of hybrids
Authors: R.E.F. Munhoz1, A.J. Prioli1, A.T. Amaral J�ºnior2, C.A. Scapim1 and G.A. Simon1
Diallel analysis was used to obtain information on combining ability, heterosis, estimates of genetic distances by random amplified polymorphic DNA (RAPD) and on their correlations with heterosis, for the popcorn varieties RS 20, UNB2, CMS 43, CMS 42, Zélia, UEM J1, UEM M2, Beija-Flor, and Viçosa, which were crossed to obtain all possib.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr592
- DOI:
- 10.4238/vol8-3gmr592