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Molecular Markers

Medical Genetics   Research Article

Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy

Authors: J. Han, W.Y. Wang, X.P. Leng, L. Guo, M.L. Yu, W.B. Jiang and R.J. Ma

One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram .. Read More»

Genet. Mol. Res. 13(1):
2014.January.8.2
DOI:
10.4238/2014.January.8.2
Animal Genetics   Research Article

DNA extraction from bristles and quills of Chaetomys subspinosus (Rodentia: Erethizontidae) using a novel protocol

Authors: C.G. Oliveira, R.A. Martinez, F.A. Gaiotto

DNA extraction protocols are as varied as DNA sources. When it comes to endangered species, it is especially important to pay attention to all details that ensure the completion of the study goals and effectiveness in attaining useful data for conservation. Chaetomys subspinosus (Rodentia: Erethizontidae) is a secretive arbore.. Read More»

Genet. Mol. Res. 6(3):
Human Genetics   Review Article

Backcross assisted by microsatellite markers in common bean

Authors: L.K. Oliveira1,3, L.C. Melo2, C. Brondani3, M.J.D. Peloso2 and R.P.V. Brondani3

The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virus and, for all crosses, only individuals resistant to the bean golden mosaic virus were back.. Read More»

Genet. Mol. Res. 7(4):
Human Genetics   Research Article

Intraspecific differentiation of Hancornia speciosa revealed by simple sequence repeat and random amplified polymorphic DNA markers

Authors: C.A. Nogueira, N.B. Stafuzza,T.P. Ribeiro, A.D.L. Prado, I.P.P. Meneze, N. Peixoto, P.J. Gon�§alves,L.M. Almeida

Hancornia speciosa, popularly known as mangabeira, is a fruit tree native to the Brazilian Cerrado that shows great economic potential, due to its multiple uses. Intraspecific classification of this species is difficult because it shows high morphological diversity. An early study of the species reported that there are six bot.. Read More»

Genet. Mol. Res. 14(4):
2015.December.7.12
DOI:
10.4238/2015.December.7.12
Plant Genetics   Research Article

Comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of Vitis vinifera

Authors: G.C. Santâ��Ana, J.L. Ferreira, H.S. Rocha, A. Bor�©m, M. Pasqual and G.M.A. Can�§ado

Identification and knowledge concerning genetic diversity are fundamental for efficient management and use of grapevine germplasm. Recently, new types of molecular markers have been developed, such as retrotransposon-based markers. Because of their multilocus pattern, retrotransposon-based markers might be able to differentiat.. Read More»

Genet. Mol. Res. 11(2):
2012.May.21.8
DOI:
10.4238/2012.May.21.8
Animal Genetics   Research Article

Does beekeeping reduce genetic variability in Melipona scutellaris (Apidae, Meliponini)?

Authors: G.A. Carvalho-Zilse, M.F.F. Costa-Pinto, C.G. Nunes-Silva, W.E. Kerr

Many factors have contributed to reductions in wild populations of stingless bees, such as: deforestation, displacement and destruction of nests by honey gatherers, as well as use of insecticides and other agrochemicals. All of these can potentially affect the populational structure of native species. We analyzed genetic varia.. Read More»

Genet. Mol. Res. 8(2):
vol8-2keer006
DOI:
10.4238/vol8-2keer006
Plant Genetics   Review Article

Potential of maize single-cross hybrids for extraction of inbred lines using the mean components and mixed models with microsatellite marker information

Authors: M. Balestre, R.G. Von Pinho, J.C. Souza and J.C. Machado

The present study examined the importance of mean (m+a’ and d) components in the performance of single-cross hybrids for the formation of new populations and determined the contribution of the mixed model (best linear unbiased predictor of random effects, BLUP) method associated with molecular markers for the choice of crosses to obtain interpopulat.. Read More»

Genet. Mol. Res. 7(4):
vol7-4gmr491
DOI:
10.4238/vol7-4gmr491
Animal Genetics   Research Article

Association of single nucleotide polymorphisms in the bovine leptin and leptin receptor genes with growth and ultrasound carcass traits in Nellore cattle

Authors: R.C.G. da Silva J.B.S. Ferraz F.V. Meirelles J.P. Eler J.C.C. Balieiro D.C. Cucco E.C. Mattos F.M. Rezende S.L. Silva

Given the important role of leptin in metabolism, we looked for a possible association of leptin and leptin receptor polymorphisms with carcass and growth traits in Nellore cattle. We examined associations of leptin and leptin receptor SNPs with ultrasound carcass (longissimus dorsi muscle area (ribeye area), backfat thickness and rump fat thickness and grow.. Read More»

Genet. Mol. Res. 11(4):
2012.August.17.10
DOI:
10.4238/2012.August.17.10
Animal Genetics   Research Article

Molecular markers in commercial Bombyx mori (Lepidoptera: Bombycidae) hybrids susceptible to multiple nucleopolyhedrovirus

Authors: L.F.C. Ribeiro D.B. Zanatta J.P. Bravo R.M.C. Brancalh�£o M.A. Fernandez

The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance.. Read More»

Genet. Mol. Res. 8(1):
vol8-1gmr540
DOI:
10.4238/vol8-1gmr540
Animal Genetics   Research Article

Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs

Authors: F.B. Britto, D.S.F. Mendes, M. Ogawa, I.H.A. Cintra and F.M. Diniz

We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of eac.. Read More»

Genet. Mol. Res. 10(4):
2011.November.9.2
DOI:
10.4238/2011.November.9.2