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Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down stream processes

Author(s): M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu, N. Na and Z. Huiyan

We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from etiolated leaves of wheat crop (Triticum aestivum). mtDNA extraction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applications such as DNA restriction, amplification, and cloning.


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