Mitochondrial Dna
Mitochondrial DNA control region analysis of three ethnic populations in lower Northern part of Thailand
Authors: U. Suyasunanont, M. Nakkuntod and S. Mirasena
The lower northern part of Thailand contains various genetically diverse ethnic populations. The sequences of the mitochondrial DNA hypervariable region were studied in three ethnic populations inhabiting Phitsanulok Province. One hundred and nine nucleotide sequences - 53, 29, and 27 from Hmongs (Hill tribe), Lao Songs, and T.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039687
- DOI:
- 10.4238/gmr16039687
Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down stream processes
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu, N. Na and Z. Huiyan
We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from etiolated leaves of wheat crop (Triticum aestivum). mtDNA extraction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applicati.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.27
- DOI:
- 10.4238/2014.December.4.27
Utility of hair shafts from study skins for mitochondrial DNA analysis
Authors: N. Kurihara
The condition of mtDNA in hair shafts preserved in a museum was examined using 30 study skins of masked palm civets, Paguma larvata (Viverridae), collected between 1924 and 2011. Comparisons of extracts from fresh and burnt alum-fixed hair shafts showed that burnt alum, which is commonly used in taxidermy, had no harmful effect on the amount of total DNA .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.1
- DOI:
- 10.4238/2013.November.11.1
Universal primers to amplify the complete mitochondrial 12S rRNA gene in marine fish species
Authors: X.X. Jin, S.L. Zhao, R.X. Wang
A pair of new universal 12S mitochondrial rRNA gene primers was designed through multiple alignment analysis of the mitochondrial tRNAPhe and the 5’ region of 16S mitochondrial rRNA genes of different kinds of fishes. The primers were successfully used to amplify an expected product fragment of about 1.2 kb from various marine fish species, and the .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.6
- DOI:
- 10.4238/2013.October.15.6
Mitochondrial and microsatellite DNA analyses showed comparative genetic diversity between parent and offspring populations of Korean black rockfish in a hatchery facility
Authors: H.S. An, J.W. Lee, J.Y. Park, J.I. Myeong and C.M. An
The black rockfish, Sebastes inermis (Sebastidae), is an important commercial fishery resource in Korea. As a preliminary investigation into the effect of artificial reproduction in a hatchery facility, the genetic divergence between parent and offspring populations of black rockfish was accessed using 10 polymorphic nuclear microsatellite DNA loci and a .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.9.2
- DOI:
- 10.4238/2013.December.9.2
Range-wide phylogeography and conservation genetics of a narrowly endemic stream salamander, Pachyhynobius shangchengensis (Caudata, Hynobiidae): implications for conservation
Authors: T. Pan, H. Wang, C.-C. Hu, W.-B. Shi, K. Zhao, X. Huang and B.-W. Zhang
The Shangcheng stout salamander (Pachyhynobius shangchengensis) is an endangered amphibian endemic to the Dabie Mountains, southeast China, and is currently threatened by habitat loss and illegal poaching. Here we used the mitochondrial DNA control region sequence (768 bp) to conduct a comprehensive investigation of genetic di.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.13.9
- DOI:
- 10.4238/2014.February.13.9
Complete sequence analysis of mitochondrial DNA of aplastic anemia patients
Authors: X. Cui, F. Liu, J.Q. Wang, W.J. Zhang, J.Y. Wang, K. Liu, S.Y. Cui, J. Zhang and R.R. Xu
This study was primarily undertaken to test the hypothesis that mitochondrial DNA (mtDNA) mutations may be associated with aplastic anemia (AA). We analyzed mtDNA sequences from 15 patients with AA. The samples were obtained from bone marrow, and patients’ oral epithelial cells were collected for normal tissue comparison. Total DNA was amplified by .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.6.16
- DOI:
- 10.4238/2012.August.6.16
Molecular barcoding of venomous snakes and species-specific multiplex PCR assay to identify snake groups for which antivenom is available in Thailand
Authors: A. Supikamolseni1,2, N. Ngaoburanawit3, M. Sumontha4, L. Chanhome5,S. Suntrarachun6, S. Peyachoknagul2,7 and K. Srikulnath1,2,7
DNA barcodes of mitochondrial COI and Cytb genes were constructed from 54 specimens of 16 species for species identification. Intra- and interspecific sequence divergence of the COI gene (10 times) was greater than that of the Cytb gene (4 times), which suggests that the former gene may be a better marker than the latter for species delimitation in snakes.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.18
- DOI:
- 10.4238/2015.October.29.18
Mitochondrial D-loop nucleotide diversity in Astyanax (Osteichthyes, Characidae) from the upper Paran�¡ and upper Paraguay River basins
Authors: A.J. Prioli, V.A. Carlo, T.V. Soria, S.M.A.P. Prioli, C.S. Pavanelli, R.A. Prioli, V.N. Gomes, H.F. J�ºlio Jr. and L.M. Prioli
The morphological discrimination between the species Astyanax altiparanae and A. asuncionensis of the upper Paraná River and Paraguay River basins, respectively, has always been difficult. Two D-loop haplogroups of A. altiparanae are known, one with the presence (AltoPR) or the absence (AltoPR-D) of a 32-bp block simila.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.5
- DOI:
- 10.4238/2012.April.27.5
Differentiation of four strains of Chinese soft-shelled turtle (Pelodiscus sinensis) based on high-resolution melting analysis of single nucleotide polymorphism sites in mitochondrial DNA
Authors: H.Q. Zhang, C. Zhang, X.J. Xu, J.J. Zhu, Z.Y. He and J.Z. Shao
The Chinese soft-shelled turtle (Pelodiscus sinensis) has been one of the most economically important aquatic animals in China for thousands of years, and several breeding strains have been formed. Since the morphological characteristics of some strains are similar, a rapid and accurate molecular method to differentiate between strains is required. In thi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.10
- DOI:
- 10.4238/2015.October.26.10