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Mitochondrial Dna

Human Genetics   Research Article

Evaluation of a method for high yield purification of largely intact mitochondrial DNA from human placentae

Authors: Sergio U. Dani, Alessandra Cristina Gomes-Ruiz, Maria Angela C. Dani

We developed, and quantitatively and qualitatively evaluated an easily reproducible method for high yield purification of mitochondrial DNA (mtDNA) from human placentae by mechanical tissue disruption, differential centrifugation of mitochondria, enzymatic digestion, phenol extraction and ethanol precipitation. Average mtDNA y.. Read More»

Genet. Mol. Res. 2(2):