New insights into the prolactin-RsaI (PRL-RsaI) locus in Chinese Holstein cows and its effect on milk performance traits
Authors: C.H. Dong1, X.M. Song2, L. Zhang2, J.F. Jiang2, J.P. Zhou1 and Y.Q. Jiang2
Prolactin (PRL) plays central roles in mammals’ reproduction, gland development, milk secretion, and the expression of milk protein genes. In dairy cattle, the PRL gene is a potential quantitative trait locus and genetic marker related to milk performance traits. Here, a total of 586 randomly selected Chinese Holstein cows were genotyped for locus P.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.3
- DOI:
- 10.4238/2013.November.22.3
In-depth pedigree analysis in a large Brazilian Nellore herd
Authors: F.V. Brito1, M. Sargolzaei2,3, J. Braccini Neto1, J.A. Cobuci1, C.M. Pimentel1, J. Barcellos1 and F.S. Schenkel2
A large herd of Nellore cattle was evaluated using in-depth pedigree analyses. Taking into account the incomplete pedigree due to the use of multiple young sires for mating, the average inbreeding coefficient was calculated as 1.73% for the last generation, which was higher than the regular inbreeding coefficient (0.25%). The effective population size was.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.2
- DOI:
- 10.4238/2013.November.22.2
Phylogenetic analysis of substrate-binding subunits in an osmoprotectant system
Authors: T.J.D. Coutinho, N.F. Rodrigues and S.T. Farias
Substrate-binding subunits are important components of the solute importation system, known as the osmoprotectant system, which consists of a membrane protein belonging to the ABC superfamily... Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.22.1
- DOI:
- 10.4238/2013.November.22.1
Gene expression during induced differentiation of sheep bone marrow mesenchymal stem cells into osteoblasts
Authors: Y. Hu, X.-X. Tang and H.-Y. He
The aim of this study was to observe the dynamic expression of osteopontin, type I collagen, and osteocalcin genes during the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts and confirm whether BMSCs can differentiate and mature into osteoblasts. A healthy, 2-month-old Altay tailed sheep was obtained, and 10 mL bone marrow was .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.4
- DOI:
- 10.4238/2013.December.11.4
A new tetra-primer ARMS-PCR for genotyping bovine kappa-casein polymorphisms
Authors: P.A.S. Fonseca, I.C. Rosse, M. DeMiranda, M.A. Machado,R.S. Verneque, M.G.C.D. Peixoto and M.R.S. Carvalho
Kappa-casein (κ-casein) is one of the most abundant milk proteins. Its main function is to avoid the aggregation of casein micelles, keeping them, and therefore calcium phosphate, in pockets in solution. In bovines, a κ-casein functional polymorphism has been associated with fat, calcium, and protein milk contents and faster curd contraction i.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.3
- DOI:
- 10.4238/2013.December.11.3
Infertility caused by male partners with genetic defects in Sichuan Province of China
Authors: Q. Quan, T.J. Li, X.P. Ding, J. Wei, L.X. Li and L. Fu
The purpose of this study was to detect chromosomal aberrations and azoospermia factor (AZF) microdeletions in male patients with reproductive problems and to summarize related clinical features to provide reliable information for evaluating prenatal and preimplantation diagnoses. A large cohort of 5083 men with various phenotypes of male infertility was .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.2
- DOI:
- 10.4238/2013.December.11.2
Random regression models using Legendre orthogonal polynomials to evaluate the milk production of Alpine goats
Authors: F.G. Silva, R.A. Torres, L.F. Brito, R.F. Euclydes, A.L.P. Melo,N.O. Souza1, J.I. Ribeiro Jr. and M.T. Rodrigues
The objective of this study was to identify the best random regression model using Legendre orthogonal polynomials to evaluate Alpine goats genetically and to estimate the parameters for test day milk yield. On the test day, we analyzed 20,710 records of milk yield of 667 goats from the Goat Sector of the Universidade Federal de Viçosa. The evaluat.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.11.1
- DOI:
- 10.4238/2013.December.11.1
Development and characterization of novel microsatellite markers in the rock bream fish Oplegnathus fasciatus
Authors: X.X. Sun, D.D. Xu, B. Lou, S.L. Li, W. Zhan, Y.R. Zhang and J. Xin
The rock bream fish Oplegnathus fasciatus is one of the most popular aquaculture species in China. In the present study, 15 novel polymorphic microsatellite loci were isolated and characterized from a wild population of O. fasciatus from the Zhoushan coast of China. The number of alleles per polymorphic locus ranged from 4 to 9 in a sample of 30 individua.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.7
- DOI:
- 10.4238/2013.December.10.7
Differences in frequencies of UGT1A9, 1A7, and 1A1 genetic polymorphisms in Chinese Tibetan versus Han Chinese populations
Authors: W. Yan, Y.W. Wang, F.F. Yang, M. Wang, X.Q. Zhang, J. Dong,E. Chen and J. Yang
As part of a series of pharmacogenomics studies of the Chinese population, we investigated genetic polymorphisms of some UGT1A regions. The three genes that were analyzed were UGT1A9, 1A7, and 1A1; we sequenced their exons, together with promoters, surrounding introns and 3'-untranslated regions (3êÂ?Â?UTR) in 100 unrelated-healthy Chinese Tibetan individua.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.6
- DOI:
- 10.4238/2013.December.10.6
Contribution of catechol-O-methyltransferase Val158Met polymorphism to endometrial cancer risk in postmenopausal women: a meta-analysis
Authors: G. Lin, J. Zhao, J. Wu, R. Andreevich O, W.-H. Zhang, Y. Zhang and L. Yu
Catechol-O-methyltransferase (COMT) is a critical enzyme to detoxify the carcinogenic catechol estrogen and the Val158Met polymorphism of COMT could influence its enzymatic activity. Recent epidemiological studies have investigated the correlation of COMT Val158Met polymorphism with endometrial cancer risk; however, the results are inconsistent. To better.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.5
- DOI:
- 10.4238/2013.December.10.5