Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR
Authors: H. Li1, D.H. Ni, Y.B. Duan, Y. Chen, J. Li, F.S. Song,L. Li, P.C. Wei and J.B. Yang
Rice false smut (RFS) is an important rice disease that is caused by the pathogen Ustilaginoidea virens. In this study, we developed a real-time polymerase chain reaction (PCR) assay to detect U. virens and to estimate the level of disease. The genomic DNAs of U. virens and rice were extracted together from the rice samples. Real-time PCR assays were perf.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.4
- DOI:
- 10.4238/2013.December.10.4
Expression and localization of the vascular endothelial growth factor and changes of microvessel density during hair follicle development of Liaoning cashmere goats
Authors: Q.L. Zhang, J.P Li, Y.M. Li, Q. Chang, Y. Chen, H.Z. Jiang, Z.H. Zhao and D. Guo
Vascular endothelial growth factors (VEGFs) play important roles in neovascularization, tissue development, and angiogenesis. In this study, changes in VEGF expression patterns and microvessel density (MVD), and their correlations, were investigated during hair follicle development in epidermal appendages of Liaoning cashmere goats. Polyclonal antibodies .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.3
- DOI:
- 10.4238/2013.December.10.3
Dietary methionine effects on IGF-I and GHR mRNA expression in broilers
Authors: A.P. Del Vesco, E. Gasparino, A.R. Oliveira Neto, S.E.F. Guimar�£es, S.M.M. Marcato and D.M. Voltolini
This study aimed to evaluate liver and breast muscle insulin-like growth factor I (IGF-I) and growth hormone receptor (GHR) gene expression between broilers fed different methionine levels and sources. Broiler chicks were 22 to 42 days old, distributed in 5 treatments (control diet, DL1 - 0.08% DL-methionine, DL2 - 0.24% DL-methionine, MHA-FA1 - 0.11% met.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.10.2
- DOI:
- 10.4238/2013.December.10.2
Evaluation of genetic diversity in Chinese kale (Brassica oleracea L. var. alboglabra Bailey) by using rapid amplified polymorphic DNA and sequence-related amplified polymorphism markers
Authors: J. Zhang and L.G. Zhang1
Chinese kale is an original Chinese vegetable of the Cruciferae family. To select suitable parents for hybrid breeding, we thoroughly analyzed the genetic diversity of Chinese kale. Random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) molecular markers were used to evaluate the genetic div.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.14.10
- DOI:
- 10.4238/2014.February.14.10
Study of the correlation between growth hormone deficiency and serum leptin, adiponectin, and visfatin levels in adults
Authors: Z.-P. Li, M. Zhang, J. Gao, G.-Y. Zhou, S.-Q. Li and Z.-M. An
We aimed to determine the significance and changes in leptin, adiponectin (ADP), and visfatin levels in adults with growth hormone deficiency (GHD). Forty adults (19 men, 21 women) who had been diagnosed with GHD comprised the observation group, while 36 healthy adults (18 men, 18 women) were used as the control group. Fasting venous blood was collected t.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.14.16
- DOI:
- 10.4238/2014.February.14.16
Agrobacterium tumefaciens-mediated transformation of Lasiodiplodia theobromae, the causal agent of gummosis in cashew nut plants
Authors: C.R. Muniz, G.F. da Silva, M.T. Souza Jr., F.C.O. Freire, G.H.J. Kema and M.I.F. Guedes
Lasiodiplodia theobromae is a major pathogen of many different crop cultures, including cashew nut plants. This paper describes an efficient Agrobacterium tumefaciens-mediated transformation (ATMT) system for the successful delivery of T-DNA, transferring the genes of green fluorescent protein (gfp) and hygromycin B phosphotra.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.21.8
- DOI:
- 10.4238/2014.February.21.8
Hydrogen peroxide enhances the uptake of polyethylenimine/oligonucleotide complexes in A549 cells by activating CaMKII independent of [Ca2+]c elevation
Authors: Z. Ma, W.W. Sun and X. Wang
Aerosol oligonucleotide therapy has vast potential in pulmonary system diseases. Reactive oxygen species (ROS) play an important role in complex physiological processes such as cell signaling, apoptosis, etc. Therefore, to determine the mechanism of ROS involvement in polyethylenimine/oligonucleotide (PEI/ON) endocytosis in ce.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.21.15
- DOI:
- 10.4238/2014.February.21.15
Significant association between lower pulse pressure and increasing levels of a novel type of phospholipid
Authors: C.S. Yao, Q.Z. Wu, H.X. Xu, F. Liu, L.G. Chang, L.C. Song, L.S. Gao and X.L. Meng
The aim of this study was to analyze the association between pulse pressure and a novel type of phospholipid with solubility similar to that of lysophosphatidic acid (LPA), designated as AP, which was reported to be elevated during ischemia. In this cross-sectional study, 416 hypertensive patients and 252 controls aged between.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.February.21.16
- DOI:
- 10.4238/2014.February.21.16
Selection of high heterozygosity popcorn varieties in Brazil based on SSR markers
Authors: I.B.O. Eloi, C.A. Mangolin, C.A. Scapim, C.S. Gon�§alves and M.F.P.S. Machado
We analyzed genetic structure and diversity among eight populations of popcorn, using SSR loci as genetic markers. Our objectives were to select SSR loci that could be used to estimate genetic diversity within popcorn populations, and to analyze the genetic structure of promising populations with high levels of heterozygosity .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.5
- DOI:
- 10.4238/2012.July.19.5
Plasma resistin, associated with single nucleotide polymorphism -420, is correlated with C-reactive protein in Chinese Han patients with spontaneous basal ganglia hemorrhage
Authors: X.-Q. Dong, Q. Du, W.-H. Yu, Z.-Y. Zhang, Q. Zhu, Z.-H. Che, H. Wang, J. Chen, S.-B. Yang and J.-F. Wen
We examined a possible relationship -420C>G SNP of the resistin gene with plasma resistin and C-reactive protein concentrations in intracerebral hemorrhage. Three hundred and forty-four Chinese Han patients with intracerebral hemorrhage and 344 age- and gender-matched healthy controls were included in our study. Plasma resi.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.19.4
- DOI:
- 10.4238/2012.July.19.4