G.Q. Zhu
Promoter identification and analysis of key glycosphingolipid biosynthesis-globo series pathway genes in piglets
Glycosphingolipid biosynthesis-globo series pathway genes (FUT1, FUT2, ST3GAL1, HEXA, HEXB, B3GALNT1, and NAGA) play an important regulatory role in the defense against Escherichia coli F18 in piglets. In this study, we identified the transcription initiation site and promoter of this gene cluster by mined previous RNA-seq results using bioinformatics tools. The FUT.. Read More»
Genet. Mol. Res. 16(2): gmr16029574
DOI: 10.4238/gmr16029574
Applying the Fisher score to identify Alzheimer’s disease-related genes
Biologists and scientists can use the data from Alzheimer's disease (AD) gene expression microarrays to mine AD disease-related genes. Because of disadvantages such as small sample sizes, high dimensionality, and a high level of noise, it is difficult to obtain accurate and meaningful biological information from gene expression profiles. In this paper, we present a .. Read More»
Genet. Mol. Res. 15(2): gmr.15028798
DOI: 10.4238/gmr.15028798
TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18
TLR4 is the main recognition receptor of bacterial lipopolysaccharides, which play an important role in innate and adaptive immunity. We used real-time PCR to analyze the tissue expression profile and differential expression of TLR4 in 4 pig populations (Escherichia coli F18-resistant Sutai, E. coli F18-sensitive Sutai, Large White, Meishan), in order to determine the role that the TLR4 gene pl.. Read More»
Genet. Mol. Res. 12(3): 2013.July.30.1
DOI: 10.4238/2013.July.30.1
Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs
The bactericidal/permeability-increasing protein (BPI) gene has been identified as a candidate gene for disease-resistance breeding. We evaluated whether polymorphisms in exons 4 and 10 of the BPI gene are associated with immune indices [interleukin-2 (IL- 2), IL-4, IL-6, interferon-b (IFN-b), IL-10, and IL-12]. In this study, we identified one mutation (C522T) in the BPI exon 4 site and two mu.. Read More»
Genet. Mol. Res. 14(2): 2015.June.8.2
DOI: 10.4238/2015.June.8.2
A new approach for cloning hLIF cDNA from genomic DNA isolated from the oral mucous membrane
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the study of life science, but it is difficult to obtain by traditional reverse transcription. We employed a novel strategy to clone human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA, which was directly isolated from the mucous membrane of mouth. The hLIF sequen.. Read More»
Genet. Mol. Res. 10(4): 2011.November.25.2
DOI: 10.4238/2011.November.25.2
Age-dependent expression of the BPI gene in Sutai piglets
We compared and analyzed expression of the BPI gene of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 by the real-time PCR method, in order to determine if it is involved in protection against disease caused by ETEC F18. There was a significant difference between 18- and 35-day expression in the jejunum. There were also significant differences between 35-day expressio.. Read More»
Genet. Mol. Res. 12(2): 2012.April.12.1
DOI: 10.4238/2012.April.12.1
Identification of BPI protein produced in different expression system and its association with Escherichia coli F18 susceptibility
The super antibiotic bactericidal/permeability-increasing (BPI) protein is a member of a new generation of proteins that have been implicated as endotoxin-neutralizing agents. In this study, recombinant porcine BPI protein was obtained by generating porcine BPI encoding prokaryotic, eukaryotic, and yeast expression vectors. Recombinant protein expression was detected in yeast GS115, Escher.. Read More»
Genet. Mol. Res. 14(1): 2015.February.6.15
DOI: 10.4238/2015.February.6.15
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