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A.F. Blank

Publications of : A.F. Blank
Plant Genetics   Research Article

Analysis of genetic diversity of Hyptis pectinata (L.) Poit. plants using ISSR markers

Hyptis pectinata, popularly known as ‘sambacaitá’ or ‘canudinho’, is a medicinal and aromatic species widely used in the Brazilian Northeast. In Sergipe, the excessive extraction of natural resources may reduce the genetic variability of native plants. Thus, molecular markers have frequently been applied to the characterization of gene.. Read More»

Genet. Mol. Res. 16(3): gmr16039603

DOI: 10.4238/gmr16039603

Human Genetics   Research Article

Analysis of genetic diversity of a native population of Myrcia lundiana Kiaersk. plants using ISSR markers

    M.F. Alves, D.A.C. Nizio, F.A. Brito, T.S. Sampaio, A.V.C. Silva, M.F. Arrigoni-Blank, S.V.A. Carvalho and A.F. Blank

Myrcia lundiana Kiaersk. is a tree of the family Myrtaceae found in tropical and subtropical areas of the southern hemisphere that produces essential oil. The aim of this study was to characterize the genetic diversity of M. lundiana plants from a native population of Parque Nacional de Itabaiana, using inter-simple sequence repeat molecular markers. Thirty-five pri.. Read More»

Genet. Mol. Res. 15(4): gmr15049198

DOI: 10.4238/gmr15049198

Cytogenomics   Research Article

Development and characterization of microsatellite primers in Pogostemon cablin (Lamiaceae)

    S.S. Sandes, J.B. Pinheiro, M.I. Zucchi, M. Monteiro, M.F. Arrigoni-Blank and A.F. Blank

Microsatellite primers were developed and optimized for patchouli (Pogostemon cablin) to characterize the patchouli Active Germplasm Bank of Universidade Federal de Sergipe. Creation of a genomic library for patchouli enabled the design of 12 microsatellite primers. Six of these microsatellites were polymorphic, revealing two well-defined groups of individuals that possess exclusive alleles. Th.. Read More»

Genet. Mol. Res. 12(3): 2013.August.8.4

DOI: 10.4238/2013.August.8.4

Human Genetics   Research Article

Cytotoxic effects of essential oils from three Lippia gracilis Schauer genotypes on HeLa, B16, and MCF-7 cells and normal human fibroblasts

    J.O. Melo, A.L. Fachin, W.F. Rizo, H.C.R. Jesus, M.F. Arrigoni-Blank, P.B. Alves, M.A. Marins, S.C. França and A.F. Blank

This study aimed to evaluate the chemical composition of the essential oils from three genotypes of Lippia gracilis Schauer (Verbenaceae) and investigate the cytotoxic activities of these oils. Essential oils were extracted from the leaves using a Clevenger-type apparatus, and chemical analysis was performed using a gas chromatograph coupled to a mass spectrometer a.. Read More»

Genet. Mol. Res. 13(2): 2014.April.8.12

DOI: 10.4238/2014.April.8.12

Human Genetics   Research Article

Analysis of genetic diversity of Laeliinae (Orchidaceae) in the State of Sergipe using ISSR markers

    M.F. Arrigoni-Blank, M.S. Santos, A.F. Blank, A.R.C. Rabbani, R. Silva-Mann, J.B. Santos, A.S. Costa and T.S.A. Menezes

The Orchidaceae represent one of the largest and most diverse families on the planet. However, this family is constantly threatened by predators and by the advancement of urban centers over its natural habitats. The objective of this study was to use inter-simple sequence repeat markers to evaluate the genetic diversity between orchid accessions of the Laeliinae sub.. Read More»

Genet. Mol. Res. 15(2): gmr.15027997

DOI: 10.4238/gmr.15027997

Plant Genetics   Research Article

Development of a novel set of microsatellite markers for Lippia alba (Verbenaceae)

    D.S. Rocha, C.P. Santos, M.M. Bajay, J.B. Campos, A.F. Blank,J.B. Pinheiro and M.I. Zucchi

Microsatellite primers were developed and optimized for Lippia alba to characterize the L. alba germplasm bank of Universidade de São Paulo. A genomic library enabled the design of 9 microsatellite primers. Six of the 9 primers yielded polymorphic products, which defined 2 groups in the bank. The data provide support to characterize germplasm banks, genetic breeding programs for L. alba, and othe.. Read More»

Genet. Mol. Res. 14(1): 2015.February.3.4

DOI: 10.4238/2015.February.3.4

Plant Genetics   Research Article

Genetic diversity of Lippia sidoides Cham. and L. gracilis Schauer germplasm

    C.P. Santos, J.B. Pinheiro, M.I. Zucchi, M.M. Bajay, J.B. Campos, M.F. Arrigoni-Blank, J.A.O. Pinto and A.F. Blank

The conservation of plants in germplasm banks ensures the characterization and availability of these resources for future generations. The present study used DNA markers to obtain genetic information about germplasm collections of Lippia sidoides and L. gracilis, which are maintained in an Active Germplasm Bank (AGB). Genetic variability of samples in the AGB was assessed using 12 combinations .. Read More»

Genet. Mol. Res. 15(3): gmr.15037781

DOI: 10.4238/gmr.15037781

Medical Genetics   Research Article

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

    F.A. Brito, D.A.C. Nizio, A.V.C. Silva2 L.E.C. Diniz, A.R.C. Rabbani, M.F. Arrigoni-Blank, S.V. Alvares-Carvalho, G.M. Figueira, I. Montanari Júnior and A.F. Blank

Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (U.. Read More»

Genet. Mol. Res. 15(3): gmr.15038681

DOI: 10.4238/gmr.15038681

Animal Genetics   Research Article

Genetic diversity of a native population of Myrcia ovata (Myrtaceae) using ISSR molecular markers

Myrcia ovata Cambess. (Myrtaceae) is a medicinal and aromatic plant that has analgesic, bactericidal and fungicidal properties. Even though this plant has economic potential, nothing is known about the variability and genetic diversity of this species. This information is necessary to establish conservation strategies and allow prospection of natural resources. The .. Read More»

Genet. Mol. Res. 17(3): http://dx.doi.org/gmr18022

DOI: http://dx.doi.org/10.4238/gmr18022

Microbial Genetics   Research Article

Molecular and chemical characterization of vetiver, Chrysopogon zizanioides (L.) Roberty, germplasm

    R.S. Celestino, M.I. Zucchi, J.B. Pinheiro, J.B. Campos, A.A. Pereira, F.G. Bianchini, R.N. Lima, M.F. Arrigoni-Blank, P.B. Alves and A.F. Blank

Due to the economic interests in vetiver, Chrysopogon zizanioides (L.) Roberty, molecular and chemical studies are essential to generate information for its sustainable exploitation. The aim of this study was to undertake a molecular and chemical characterization of vetiver accessions of the active germplasm bank of the Universidade Federal de Sergipe. The molecular characteristics of the acces.. Read More»

Genet. Mol. Res. 14(3): 2015.August.14.9

DOI: 10.4238/2015.August.14.9