Microbial Genetics
Genetic parameters and breeding strategies for high levels of iron and zinc in Phaseolus vulgaris L.
Authors: S.M. Martins, P.G.S. Melo, L.C. Faria, T.L.P.O. Souza, L.C. Melo and H.S. Pereira
One of the current focus of common bean breeding programs in Brazil is to increase iron (FeC) and zinc content (ZnC) in grains. The objectives of this study were to estimate genetic parameters for FeC and ZnC in common bean, verify the need for conducting multi-site evaluation tests, identify elite lines that combine high FeC .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028011
- DOI:
- 10.4238/gmr.15028011
In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops
Authors: C. Bredow, J.L. Azevedo, J.A. Pamphile, C.A. Mangolin and S.A. Rhoden
Because of human population growth, increased food production and alternatives to conventional methods of biocontrol and development of plants such as the use of endophytic bacteria and fungi are required. One of the methods used to study microorganism diversity is sequencing of the 16S rRNA gene, which has several advantages, including universality, size.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.3
- DOI:
- 10.4238/2015.August.19.3
Isolation and characterization of a novel paraffin wax-degrading bacterium, Pseudomonas sp strain PW-1, from petroleum-contaminated sites
Authors: Y.L. Zhang, Z. Liu, and T. Liu
An isolate capable of degrading paraffin wax was isolated from petroleum-contaminated sites in Daqing, China, and identified as Pseudomonas sp strain PW-1 by analyzing the 16S rDNA sequence (GenBank accession No.: KF529529) as well as the biochemical and physiological characteristics. The optimized degradation conditions of th.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028021
- DOI:
- 10.4238/gmr.15028021
Relationship between zinc and the growth and development of young children
Authors: S. Gao, D.N. Tu, H. Li, X. Cao, J.X. Jiang, Y. Shi, X.Q. Zhou1and J.B. You
The purpose of this study was to evaluate the relationship between zinc and the growth and development of young children. The parents of 8102 young children were surveyed in person by a trained surveyor using structured questionnaires. The hair zinc concentration of the children was determined using an atomic absorption spectrophotometer. The height, weig.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.5
- DOI:
- 10.4238/2015.August.19.5
Effects of acupotomylysis on basic fibroblast growth factor and CD34 levels in rabbits with third lumbar vertebral transverse foramen syndrome
Authors: X.-H. Li, N.-G. Liu, C.-Q. Guo, H.-M. Sun, H.-X. Wu, H. Xu and Y. Zhang
This study observed the local tissue homogenates in rabbits with third lumbar vertebral transverse foramen syndrome and explored the mechanism of acupotomylysis in local tissue revascularization. Thirty Japanese white rabbits were randomly divided into the following 5 groups of 6 rabbits each: normal, model, acupotomy, electroacupuncture (EA), and acupoto.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.6
- DOI:
- 10.4238/2015.August.19.6
Identification of target genes for adenohypophysis-prefer miR-7 and miR-375 in cattle
Authors: B. Yuan, G.J. Sun, G.L. Zhang, J. Wu, C. Xu, L.S. Dai, J. Chen, X.F. Yu, Z.H. Zhao and J.B. Zhang
In this study, expression levels of miRNAs (miRNAs), miR-375 and miR-7, were detected in different tissues of cattle to determine whether adenohypophysis-prefer or exclusively expressed miRNAs, and target genes could be predicted by TargetScan, RNA22, and other software. Target genes related to pituitary function or reproductive traits were identified usi.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.8
- DOI:
- 10.4238/2015.August.19.8
Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library
Authors: Y.H. Gao, K.F. Wang, S. Zhang, Y.N. Fan, W.J. Guan and Y.H. Ma
Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expressio.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.9
- DOI:
- 10.4238/2015.August.19.9
Population structure and genetic diversity in Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae): is there a correlation between genetic and geographic proximity?
Authors: A.L.S. Mascarenhas, A.M. Waldschmidt and J.C. Silva Jr.
Gynaikothrips uzeli (Thysanoptera: Phlaeothripidae) is a minuscule insect species, which forms galls, is subsocial, and parthenogenetic. It is associated with Ficus benjamina L. (Moraceae) and has a pantropical occurrence. The paucity of genetic studies on the order Thysanoptera led us to use inter-simple sequence repeat molecular marker to assess intra- .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.12
- DOI:
- 10.4238/2015.August.19.12
Improving production of extracellular proteases by random mutagenesis and biochemical characterization of a serine protease in Bacillus subtilis S1-4
Authors: X.C. Wang, H.Y. Zhao, G. Liu, X.J. Cheng and H. Feng
The feather is a valuable by-product with a huge annual yield produced by the poultry industry. Degradation of feathers by microorganisms is a prerequisite to utilize this insoluble protein resource. To improve the degrading efficiency of feathers, mutagenesis of the bacterium Bacillus subtilis S1-4 was performed. By combining.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027831
- DOI:
- 10.4238/gmr.15027831
Genetic markers for detection of Escherichia coli K-12 harboring ampicillin-resistance plasmid from an industrial wastewater treatment effluent pond
Authors: G.A.R. Sim�µes, M.A.S. Xavier, D.A. Oliveira, E.V. Menezes,S.S.G. Magalh�£es, J.A.C.D. Gandra and A.R.E.O. Xavier
Biotechnology industries that use recombinant DNA technology are potential sources for release of genetically modified organisms to the environment. Antibiotic-resistance marker genes are commonly used for recombinant bacteria selection. One example is the marker gene coding for β-lactamase (bla) in plasmids found in Esch.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028528
- DOI:
- 10.4238/gmr.15028528