Microbial Genetics
Molecular characterization and functional analysis of the Nep1-like protein-encoding gene from Phytophthora capsici
Authors: B.Z. Feng and P.Q. Li
Phytophthora capsici is an aggressive plant pathogen that affects solanaceous and cucurbitaceous hosts. Nep1-like proteins (NLPs) are a group of effectors found particularly in oomycetes and considered important virulence factors. We identified an NLP gene (phcnlp1) from the highly virulent P. capsici strain Phyc12 that had an encoded polypeptide of 476-a.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.26.8
- DOI:
- 10.4238/2013.April.26.8
Genes associated with disc degeneration identified using microarray gene expression profiling and bioinformatics analysis
Authors: Y. Chen, K. Chen, M. Li, C. Li, H. Ma, Y.S. Bai, X.D. Zhu and Q. Fu
Disc degeneration is strongly associated with back or neck pain, sciatica, and disc herniation or prolapse. It places an enormous economic burden on society and can greatly affect quality of life. Alternative treatment approaches, such as genetic therapies, are urgently needed to slow or reverse the disc degeneration process. We downloaded gene expression.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.26.5
- DOI:
- 10.4238/2013.April.26.5
Bos taurus papillomavirus activity in peripheral blood mononuclear cells: demonstrating a productive infection
Authors: T.C. Melo, R.P. Araldi, N.S.D. Pessoa, P.L. de-S�¡-J�ºnior, R.F. Carvalho, W. Be�§ak and R.C. Stocco
Bovine papillomavirus (BPV) is an oncogenic virus with mucous and epithelial tropism. Possible productive virus infection in other tissues, such as blood, has been hypothesized. In order to investigate this possibility, three samples of skin papillomas and blood were collected from bovines with BPV infection and five samples of peripheral blood and one sa.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.19
- DOI:
- 10.4238/2015.December.11.19
Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum
Authors: L.S.A. Gon�§alves, R. Rodrigues, M.S.S. Diz, R.R. Robaina, A.T. do Amaral J�ºnior, A.O. Carvalho and V.M. Gomes
Pathogenesis-related proteins (PRs) are among the defense mechanisms of plants that work as an important barrier to the development of pathogens. These proteins are classified into 17 families according to their amino acid sequences, serology, and/or biological or enzyme activity. The present study aimed to identify PRs associated with the pathosystem of .. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.26.3
- DOI:
- 10.4238/2013.April.26.3
Differential expression of lipid metabolism genes in the liver and adipose tissue of mice treated with evodiamine
Authors: D.F. Jiang, X.G. Zhang, H.L. Yang and C. Sun
Evodiamine is the main active alkali of Wu Zhuyu, a widely distributed Chinese herb. It plays an important role in the regulation of body fat deposition. In this study, we aimed to investigate the effect of evodiamine administration on the expression of genes involved in lipid metabolism in the liver and adipose tissue. Fasted mice were subcutaneously inj.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.January.4.13
- DOI:
- 10.4238/2013.January.4.13
Mutation profile of KRAS and BRAF genes in patients with colorectal cancer: association with morphological and prognostic criteria
Authors: M. Samara, K. Kapatou, M. Ioannou, Ã?Â?. Kostopoulou, R. Papamichali, C. Papandreou, A. Athanasiadis and G. Koukoulis
KRAS and BRAF mutations are well-recognized molecular alterations during colorectal carcinogenesis, but there is little agreement on their effect on tumor characteristics. Therefore, we aimed to evaluate the distribution of the most common KRAS and BRAF mutations in Greek patients with colorectal cancer and their possible associations with clinical histop.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.6
- DOI:
- 10.4238/2015.December.14.6
Translation of foot-and-mouth disease virus RNA: factors influencing alternative AUG selection
Authors: J.L. Liu, Y.H. Ma, J.Y. Xu, Y.X. Chen, S.E. Chen and Z.R. Ma
The mechanism of alternative AUG usage in foot-and-mouth disease virus is not completely understood. Using simple computational approaches, we evaluated the contributions of overall codon bias, quantitative codon bias, and %GC of the region between the two alternative AUGs, Region-La, as well as the nucleotide bias of the sequence context flanking each AU.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.7
- DOI:
- 10.4238/2015.December.14.7
Absence of the BaeR protein leads to the early initiation of DNA replication in Escherichia coli
Authors: Y. Yao, Wunier and Morigen
Escherichia coli cells have dozens of two-component systems to sense and respond to various stimuli, and thereby cope with changing environments. BaeS/BaeR is one such two-component system, and it deals with a variety of envelope stresses. Interestingly, the ArcA/ArcB and TorS/TorR two-component systems are known to be associated with initiation of DNA re.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.16
- DOI:
- 10.4238/2015.December.14.16
Association of a let-7 KRAS rs712 polymorphism with the risk of breast cancer
Authors: X. Huang, Y. Yang, Y. Guo, Z.L. Cao, Z.W. Cui, T.C. Hu and L.B. Gao
Breast cancer (BC) is a common malignancy affecting women, with increasing incidences of this disease in China every year. Recent studies have extensively investigated a single nucleotide polymorphism in the let-7 miRNA binding site of the 3'-untranslated region of KRAS mRNA. The aim of this study was to determine the genotype frequency of the KRAS rs712 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.19
- DOI:
- 10.4238/2015.December.14.19
Cloning, characterization, and expression of cadmium-induced metallothionein-2 gene from earthworm Pheretima aspergillum (E. Perrier)
Authors: L. Gong, W. Li, J. Li, W.E. Li, W.R. Wu and L.W. Yu
Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. asperg.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.5
- DOI:
- 10.4238/2015.December.14.5