Microbial Genetics
Molecular cloning and tissue expression analyses of two novel pepper genes: heterotrimeric G protein beta 2 subunit and ArcA1
Authors: M.H. Deng, J.L. Huo, H.S. Zhu, H. Zhou and J.F. Wen
We isolated two transcription factor genes, heterotrimeric G protein beta 2 subunit (Gβ2) and ArcA1, from pepper (Capsicum annuum). The complete coding sequences were amplified using reversed transcriptase PCR based on conserved sequence information of Solanum lycopersicum and several other plant species. Nucleotide sequence analysis of these two gen.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.1
- DOI:
- 10.4238/2013.April.17.1
Subtyping Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis
Authors: M.P.S. Barros, V.M. Silveira-Filho, R.H.F.B. Lins, M.B.M. Oliveira, A.M.P. Almeida and T.C. Leal-Balbino
We subtyped Brazilian Yersinia pestis strains by pulsed-field gel electrophoresis (PFGE). This was done with 22 Brazilian Y. pestis strains: 17 from an outbreak and 5 from endemic routine surveillance. The strains were divided into 2 groups (I and II), 8 subgroups (A-H) and 19 PFGE profiles or pulsotypes. PFGE did not separate outbreak from non-outbreak s.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.January.4.23
- DOI:
- 10.4238/2013.January.4.23
Isolation and characterization of microsatellite markers in Ruditapes philippinarum
Authors: Z.B. Li, Y.F. Ning, J.B. Shangguan, B.B. Li, X.Q. Mao, Y.S. Huang and Y. Yuan
Ruditapes philippinarum is considered a commercially valuable species, which is commonly found in tidal flats along West Pacific coasts. In China, it is mainly distributed in the southeast sea. In this study, 16 novel microsatellite loci from the R. philippinarum genome were developed, using the protocol of fast isolation by a.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027742
- DOI:
- 10.4238/gmr.15027742
Smallest critical region for microcephaly in a patient with mosaic ring chromosome 13
Authors: P.-H. Su, C.-P. Chen, Y.-N. Su, S.-J. Chen, L.-L. Lin and J.-Y. Chen
A ring chromosome 13 or r(13) exhibits breakage and reunion at breakage points on the long and short arms of chromosome 13, with deletions of the chromosomal segments distal to the breakage points. The r(13) chromosome accounts for approximately 20% of ring chromosomes compatible with life. We describe a female patient with mental retardation, growth reta.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.2
- DOI:
- 10.4238/2013.April.25.2
Prevalence of CYP2B6 polymorphisms in Argentinians: the role of genetic testing
Authors: P. Scibona, C. Vazquez, A.R. Cajal, P.F. Argibay and W.H. Belloso
CYP2B6 is a highly polymorphic isoenzyme involved in the metabolism of many drugs including cyclophosphamide, bupropion, and efavirenz. A single nucleotide polymorphism (SNP) in CYP2B6 (516G>T) resulted in decreased expression and function associated with the CYP2B6*6 haplotype. Among the clinical implications of this phenotype, decreased activation of.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.6
- DOI:
- 10.4238/2015.December.11.6
Association of the rs7903146 and rs12255372 polymorphisms in the TCF7L2 gene with type 2 diabetes in a population from northeastern Brazil
Authors: C.M.A.R. Barros, A.P. Araujo-Neto, T.R. Lopes, M.A.L. Barros, F.J.N. Motta, R. Canalle, L.C.C. Nunes, J.A. Rey, R.R. Burbano, M.A. Lima-Barros, F.K.N. Yoshioka and G.R. Pinto
Approximately 200 million people suffer from type 2 diabetes (T2D) worldwide, and the rapid increase in the prevalence of this disease is likely a result of multiple environmental factors, such as increased food intake and decreased physical activity in genetically predisposed individuals. Different population studies have dem.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.1
- DOI:
- 10.4238/2014.September.29.1
Effect of isodillapiole on the expression of the insecticide resistance genes GSTE7 and CYP6N12 in Aedes aegypti from central Amazonia
Authors: V.S. Lima, A.C. Pinto and M.S. Rafael
The yellow fever mosquito Aedes (Stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. Dengue prevention measures for the control of A. aegypti involve mainly the use of synthetic insecticides. The constant use of insecticides has caused resistance in this mosquito. Alternative studies on plant extracts and their products have b.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.11.20
- DOI:
- 10.4238/2015.December.11.20
Cellulolytic activity and structure of symbiotic bacteria in locust guts
Authors: L.-J. Su, H. Liu, Y. Li, H.-F. Zhang, M. Chen, X.-H. Gao, F.-Q. Wang and A.-D. Song
Locusts are able to digest the cellulose of Gramineae plants, resulting in their being considered as major crop pests. To illustrate the mechanism involved in cellulose digestion, the cellulolytic activity and zymography in the gut contents of 16 locust species were determined using carboxymethyl cellulose (CMC) as substrate. .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.29.6
- DOI:
- 10.4238/2014.September.29.6
Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep
Authors: L.H. Wang, W. Zhang, J.L. Ji, Q.X. Gao, S.H. Xiao and F. Wang
Liver receptor homologue 1 (Lrh-1) is a member of the nuclear receptor belonging to the second subfamily of the nuclear receptor family 5A (NR5A), also named NR5A2, which is important for lipid homeostasis, embryogenesis, and regulation of aromatics. The present study aimed to understand the sequence of ovine Lrh-1 and the expression traits in reproductiv.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.6.2
- DOI:
- 10.4238/2013.May.6.2
Gene expression profile and enrichment pathways in different stages of bladder cancer
Authors: Z.-Q. Fang, W.-D. Zang, R. Chen, B.-W. Ye, X.-W. Wang, S.-H. Yi, W. Chen, F. He and G. Ye
Bladder cancer is a highly heterogeneous neoplasm. We examined the gene expression profile in 3 bladder cancer stages (Ta, T1, T2) using expression microarray analysis of 40 bladder tumors. Differentially expressed genes were found by the t-test, with <0.005 as the significance threshold. KEGG pathway-enrichment analysis was used to study the signaling.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.6.1
- DOI:
- 10.4238/2013.May.6.1