Microbial Genetics
Effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5
Authors: Z.R. Xu, F.G. Jiang and F. Chen
The OPTN gene is thought to be associated with certain types of glaucoma and the function of the protein for which it encodes, optineurin, has been extensively researched, but with contradictory results. We explored the effects of abnormal optineurin expression on the survival of the rat retinal ganglion cell line RGC-5. Plasmids expressing wild-type (WT).. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.7.27
- DOI:
- 10.4238/2015.August.7.27
Isolation, identification, and optimal cultivation of a marine bacterium antagonistic to Magnaporthe grisea
Authors: W.J. Zhang, P. Guo, M. Liu, B.L. Yang, J.H. Wang and J. Jiang
In this paper, a plate confrontation method was used to isolate bacteria antagonistic to the rice blast fungus Magnaporthe grisea from samples collected from China’s Dalian Bay. The antagonist strain LM-031 was obtained. We studied this strain’s morphological, physiological and biochemical characteristics and analy.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028646
- DOI:
- 10.4238/gmr.15028646
Genetic diversity and taxonomic status of Gymnocypris chilianensis based on the mitochondrial DNA cytochrome b gene
Authors: J.P. Zhang, Z. Liu, B. Zhang, X.Y. Yin, L. Wang, H.N. Shi and Y.J. Kang
In order to study the genetic diversity and taxonomic status of Gymnocypris chilianensis on a molecular level, the mitochondrial DNA cytochrome b gene was sequenced for 74 individuals of G. chilianensis from two locations (Heihe River and Shule River) and 42 individuals of its affinis species Gymnocypris przewalskii. Analyses of genetic diversity and sequ.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.10.5
- DOI:
- 10.4238/2015.August.10.5
Significance of Bcl10 gene mutations in the clinical diagnosis of MALT-type ocular adnexal lymphoma in the Chinese population
Authors: J. Zhu, R.L. Wei, Y.L. Pi and Q. Guo
We investigated the expression of Bcl10 gene mutations in mucosa-associated lymphoid tissue-type ocular adnexal lymphoma (OAL), atypical lymphoid hyperplasia (ALH), and reactive lymphoid hyperplasia (RLH) in the Chinese population and its role in clinical diagnosis and pathogenesis. Forty-three samples were collected during patient surgeries. Pathological.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.12.6
- DOI:
- 10.4238/2013.April.12.6
Mutational analyses of the TSC1 and TSC2 genes in cases of tuberous sclerosis complex in Chinese Han children
Authors: G.-X. Wang, D.-W. Wang, C.-Y. Yi, J.-S. Qu and Y.-L. Wang
Tuberous sclerosis complex (TSC) is an autosomal dominant neurogenetic disorder characterized by hamartomas in multiple organs and is caused by a wide spectrum of mutations in 1 of 2 causative genes (TSC1 or TSC2). Here, we present mutational analyses of the TSC1 and TSC2 genes in 4 cases of TSC in Chinese Han children, including 2 familial and 2 sporadic.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.12.3
- DOI:
- 10.4238/2013.April.12.3
Expression and diagnostic value of proteins in Mycobacterium tuberculosis
Authors: Z.Y. Zhu, Y. Liu, H.B. Wang, J.Z. Xiao, Y.F. Qiu, L. Yan, D. Chen, A.G. Liu and X. Yang
We constructed a prokaryotic expression vector expressing the Mycobacterium tuberculosis protein TB16.3, as well as 3 other proteins, including TB15.3, CFP-10, and Rv2626C, which were purified and analyzed for their effectiveness as detection antibodies. The TB16.3 genes of M. tuberculosis H37Rv geno.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.26.16
- DOI:
- 10.4238/2014.September.26.16
DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa)
Authors: C.Y. Zhang, N.N. Wang, Y.H. Zhang, Q.Z. Feng, C.W. Yang and B. Liu
Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.5
- DOI:
- 10.4238/2013.April.17.5
Impacts of single nucleotide polymorphisms and haplotypes in the bovine Dapper1 gene on body weight
Authors: J. Wang, C. Wang, Y. Gao, X.-Y. Lan, C.-Z. Lei, J.-Q. Wang and H. Chen
The Dapper1 protein plays important role in multiple developmental processes via negative modulation of the Wnt signaling pathway. We detected variations in Dapper1 in 1185 individuals from 5 Chinese cattle breeds and determined their effects on bovine body weight. Two silent mutations (g.8344C>T and g.8428C>T) in exon 6 along with two substitutions.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.4
- DOI:
- 10.4238/2013.April.17.4
Construction of overexpression vectors of Magnaporthe oryzae genes BAS1 and BAS4 fusion to mCherry and screening of overexpression strains
Authors: M.L. Liang, J.L. Yan, Y.Q. Yang, L. Liu, C.Y. Li and J. Yang
The aim of this study was to construct overexpression vectors and selecting strains of the Magnaporthe oryzae effectors BAS1 and BAS4. Primer pairs of BAS1, BAS4, and mCherry were designed based on their known nucleotide sequences. The coding sequences of BAS1 and BAS4 were amplified, and the pXY201 plasmid was selected as a template to amplify the mCherr.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.26.17
- DOI:
- 10.4238/2015.June.26.17
A novel variant of the �²-lactamase ADC-61 gene in multi-drug resistant Acinetobacter baumannii
Authors: Y. Zhou, S.-J. Teng, L. Yang, S.-B. Li and Y. Xu
The aim of this study was to investigate the existence of a β-lactamase gene in a group of multi-drug resistant Acinetobacter baumannii. Twenty strains of multi-drug resistant A. baumannii were isolated. Thirty-four β-lactamase genes and the ISaba1- OXA-23 linkage were analyzed in these strains by polymerase chain reaction (PCR) and verified by .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.2
- DOI:
- 10.4238/2015.June.29.2