Microbial Genetics
Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses
Authors: A.C. Leme, M.R.R. Bevilaqua, S.A. Rhoden, C.A. Mangolin, M.F.P.S. Machado and J.A. Pamphile
This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.11
- DOI:
- 10.4238/2013.September.27.11
Identification of a novel human testicular interstitial gene, RNF148, and its expression regulated by histone deacetylases
Authors: Y.Q. Liu, D.C. Tao, S.Y. Liao, Y. Yang, Y.X. Ma and S.Z. Zhang
Multiple genes are restrictively expressed in mammalian testicular tissues, and they play important roles in the complex process of spermatogenesis. Investigation of these genes and their expression regulation mechanisms is valuable to elucidate the molecular process of spermatogenesis. In this study, we identified a novel human gene, ring finger protein .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.7
- DOI:
- 10.4238/2013.September.27.7
Comparison of factor-analytic and reduced rank models for test-day milk yield in Gyr dairy cattle (Bos indicus)
Authors: R.J. Pereira, D.R. Ayres, L. El Faro, R.S. Verneque, A.E. Vercesi Filho and L.G. Albuquerque
We analyzed 46,161 monthly test-day records of milk production from 7453 first lactations of crossbred dairy Gyr (Bos indicus) x Holstein cows. The following seven models were compared: standard multivariate model (M10), three reduced rank models fitting the first 2, 3, or 4 genetic principal components, and three models considering a 2-, 3-, or 4-factor .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.5
- DOI:
- 10.4238/2013.September.27.5
Development and characterization of new microsatellite markers of Fenneropenaeus penicillatus
Authors: Y. Yuan,Z.B. Li1, Y.F. Ning, H.W. Deng, J.B. Shangguan, Y.S. Huang and G. Dai
Thirteen new polymorphic microsatellite markers in Fenneropenaeus penicillatus were isolated and characterized. The polymorphism of the thirteen microsatellite markers was tested by 30 individuals from Lianjiang, China. It showed that the number of alleles per locus ranged from 3 to 6 and the Polymorphism Information Content (PIC) was from 0.324 to 0.706... Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.11
- DOI:
- 10.4238/2015.June.18.11
Detection of drug-resistance mechanism of Pseudomonas aeruginosa developing from a sensitive strain to a persister during carbapenem treatment
Authors: J.L. Shen and Y.P. Fang
We explored the mechanism of the development from sensitivity to resistance to carbapenem in Pseudomonas aeruginosa. Two P. aeruginosa strains were collected during treatment with carbapenem. Strain homology was investigated using pulsed-field gel electrophoresis. Porin oprD2 expression was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophor.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.16
- DOI:
- 10.4238/2015.June.18.16
Morphological and molecular characteristics do not confirm popular classification of the Brazil nut tree in Acre, Brazil
Authors: P.S. Sujii, E.T.M.B. Fernandes, V.C.R. Azevedo, A.Y. Ciampi, K. Martins and L.H. de O. Wadt
In the State of Acre, the Brazil nut tree, Bertholletia excelsa (Lecythidaceae), is classified by the local population into two types according to morphological characteristics, including color and quality of wood, shape of the trunk and crown, and fruit production. We examined the reliability of this classification by comparing morphological and molecula.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.3
- DOI:
- 10.4238/2013.September.27.3
Effect of two single nucleotide polymorphisms on milk yield and composition
Authors: J.C. Rinc�³n, A. L�³pez-Herrera and J.J. Echeverri
The bovine prolactin (PRL) and bovine growth hormone (bGH) genes exhibit several polymorphisms. Some of them can be detected by molecular techniques using restriction endonucleases, such as RsaI for the PRL gene and MspI for the bGH gene. We examined the relationship between the PRL-RsaI and bGH-MspI polymorphisms and some economically important character.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.15
- DOI:
- 10.4238/2013.April.2.15
Cytogenetic analysis of Triatoma pseudomaculata CorrÃ?ªa and EspÃ?Ânola, 1964 (Hemiptera, Triatominae) from different Brazilian states
Authors: C.H.L. Imperador, K.C.C. Alevi, J. Oliveira, J.A. Rosa and M.T.V. Azeredo-Oliveira
Triatoma maculata and T. pseudomaculata are allopatric; however, it is believed that T. maculata was introduced into the Brazilian northeast by passive transportation of the nymphs between the feathers of migratory birds, followed by the speciation of T. pseudomaculata. T. pseudomaculata is the second most epidemiologically important species in the northe.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15016937
- DOI:
- 10.4238/gmr.15016937
Investigation of Sclerotinia sclerotiorum strains variability in Brazil
Authors: M.J. Abreu and E.A. Souza
White mold is a common bean disease caused by the fungus Sclerotinia sclerotiorum, resulting in economic losses in Brazil and worldwide. Lack of knowledge about the population structure of the pathogen makes it difficult to control the disease. The aim of this study was to characterize strains of S. sclerotiorum obtained from experimental and commercial c.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.31
- DOI:
- 10.4238/2015.June.18.31
Antimicrobial activity and cytotoxicity of endophytes from Scapania verrucosa Heeg.
Authors: J.-G. Wu, W. Peng, P.-Y. Zeng, Y.-B. Wu1, J. Yi and J.-Z. Wu
We evaluated the antibacterial activity and cytotoxicity of endophytes isolated from Scapania verrucosa Heeg., which belongs to the liverwort class. A total of 49 endophytic fungi were isolated from S. verrucosa and classified into seven genera and one family in our previous study. In this study, the cytotoxic activity of the endophytes was assessed using.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.8
- DOI:
- 10.4238/2013.April.2.8