Microbial Genetics
Multiple abnormalities due to a nonsense mutation in the Alx4 gene
Authors: B. Chen, Lu Chen, Y. Zhou, T. Mi, D.Y. Chen, Li Chen, J. Yin and Z.F. Xue
Patterning of the limb anterior-posterior axes depends on several signals that derive from the three signaling centers of the limb bud. These signals interact to constitute a complex and ordered network that critically contributes to the development of limb buds. Preaxial polydactyly in mouse is predominantly caused by ectopic expression of the zone of po.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.2.2
- DOI:
- 10.4238/2013.August.2.2
Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle
Authors: R.C. Gomes, S.L. Silva, M.E. Carvalho, F.M. Rezende, L.F.B. Pinto, M.H.A. Santana, T.R. Stella, F.V. Meirelles, P. Rossi J�ºnior, P.R. Leme and J.B.S. Ferraz
We looked for possible associations of SNPs in genes related to protein turnover, with growth, feed efficiency and carcass traits in feedlot Nellore cattle. Purebred Nellore bulls and steers (N = 290; 378 ± 42 kg body weight, 23 months ± 42 days old) were evaluated for daily feed intake, body weight gain (BWG), gross feed efficiency, feed co.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.8
- DOI:
- 10.4238/2013.August.12.8
Agrobacterium tumefaciens-mediated genetic transformation of the Taxol-producing endophytic fungus Ozonium sp EFY21
Authors: L. Liu, Y.M. Wei, X.W. Zhou, J. Lin, X.F. Sun and K.X. Tang
An efficient Agrobacterium tumefaciens-mediated genetic transformation method was successfully established for a newly isolated Taxol-producing fungus, Ozonium sp EFY21. A specific hygromycin B resistance expression vector, pCAMBIA1304’AN7-1, was constructed for fungal transformation. Key factors affecting transformation efficiency were thoroughly i.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.7
- DOI:
- 10.4238/2013.August.12.7
Analysis of fusion gene expression in prostate tumors by using single-end reads
Authors: D.D. Xie, J.Y. Li, Y. Wang, L. Chen and D.X. Yu
Fusion gene expression, a kind of chromosome rearrangement mode, has been strongly linked to prostate cancer diagnosis and prognosis as well as to the Gleason score and the American Joint Committee on Cancer stage assessment. In combination with traditional methods for locating fusion genes and scoring their association with cancer cell growth, proliferat.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.4
- DOI:
- 10.4238/2013.August.12.4
Use of molecular markers to compare Fusarium verticillioides pathogenic strains isolated from plants and humans
Authors: S.C. Chang, D.P.C. Mac�ªdo, C.M. Souza-Motta and N.T. Oliveira
Fusarium verticillioides is a pathogen of agriculturally important crops, especially maize. It is considered one of the most important pathogens responsible for fumonisin contamination of food products, which causes severe, chronic, and acute intoxication in humans and animals. Moreover, it is recognized as a cause of localized infections in immunocompete.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.12.2
- DOI:
- 10.4238/2013.August.12.2
Characteristics of synonymous codon usage bias in the beginning region of West Nile virus
Authors: X.X. Ma*, Y.P. Feng*, J.L. Liu, L. Chen, Y.Q. Zhao, P.H. Guo, J.Z. Guo, L.Y. Ma, and Z.R. Ma
Adaptation in the overall codon usage pattern of West Nile virus (WNV) to that of two hosts was estimated based on the synonymous codon usage value (RSCU). Synonymous codon usage biases for the beginning coding sequence of this virus were also analyzed by calculating the usage fluctuation for each synonymous codon along the ta.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.5.13
- DOI:
- 10.4238/2014.September.5.13
Preparation of monoclonal antibody of anti-feline calicivirus and establishment of double-antibody sandwich enzyme-linked immunosorbent assay detecting method
Authors: B. Yuan, C.-X. Ai, L. Yuan, W. Gao, J.-P. Hu, J. Chen and W.-Z. Ren
This study aimed to prepare monoclonal antibody of feline calicivirus (FCV) and identify its basic biological characteristics. Saturated ammonium sulfate precipitation, combined differential centrifugation, and cesium chloride density gradient centrifugation were used for the purification of FCV. The purified FCV was used as a.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.4
- DOI:
- 10.4238/2014.September.12.4
Expression and serological diagnosis of Mycobacterium tuberculosis CFP-10 and Rv2626c proteins
Authors: Z.Y. Zhu, D. Zhang, H.B. Wang, J.Z. Xiao, Y.F. Qiu, L. Yan, D. Chen, A.G. Liu and X. Yang
We constructed a Mycobacterium tuberculosis vector expressing CFP-10 and Rv2626c to examine the expression of these proteins in Escherichia coli as well as their immunoreactivity. The CFP-10 and Rv2626c genes were amplified from tuberculosis H37Rv genomic DNA using polymerase chain reaction. They were ligated.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.5
- DOI:
- 10.4238/2014.September.12.5
Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky)
Authors: S.-L. Liu, Z.-M. Gu, Y.-Y. Jia, J.-L. Zhao, W.-P. Jiang, Q. Li and F. Li
The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)12 and (GA)12 probes. Th.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.14
- DOI:
- 10.4238/2014.September.12.14
Screening the first set of polymorphic microsatellite loci in Lunella coronata granulata (Turbinidae)
Authors: Z.B. Li, Q.H. Li, J.B. Shangguan, Y.F. Ning and G. Dai
Lunella coronata granulata, from the family Turbinidae, is an economically important species. The first set of 10 polymorphic microsatellite loci was screened from L. coronata granulata, and 30 individuals were used to analyze the degree of polymorphism in these loci. The level of observed and expected heterozygosity was 0.0667-0.7333 and 0.0644-0.6628, r.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.6
- DOI:
- 10.4238/2015.June.11.6