Microbial Genetics
BacPP: a web-based tool for Gram-negative bacterial promoter prediction
Authors: S. de Avila e Silva, D.L. Notari, F.A. Neis, H.G. Ribeiro and S. Echeverrigaray
Bacterial Promoter Prediction (BacPP) is a tool used to predict given sequences as promoters of Gram-negative bacteria according to the σ factor that recognizes it. The first version of BacPP was implemented in Python language in a desktop version without a friendly interface. For this reason, a web version of BacPP is n.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027973
- DOI:
- 10.4238/gmr.15027973
AMFR gene silencing inhibits the differentiation of porcine preadipocytes
Authors: C.Z. Chen, Y.N. Zhu, M.L. Chai, L.S. Dai, Y. Gao, H. Jiang, L.J. Zhang, Y. Ding, S.Y. Liu, Q.Y. Li, W.F. Lu and J.B. Zhang
Our study clarifies the role of the autocrine motility factor receptor (AMFR) gene in porcine preadipocyte differentiation. AMFR-siRNA was transfected into porcine preadipocytes and the preadipocytes were induced to differentiation. Subsequently, qRT-PCR was conducted to examine changes in mRNA expression of a series of genes .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027354
- DOI:
- 10.4238/gmr.15027354
Description of the diploid chromosome set of Triatoma pintodiasi (Hemiptera, Triatominae)
Authors: K.C.C. Alevi, F.F.F. Moreira, J. Jurberg and M.T.V. Azeredo-Oliveira
Triatoma pintodiasi has been described and recently grouped in the Rubrovaria subcomplex. T. pintodiasi was initially compared to T. carcavalloi by staining and subsequently identified as T. circummaculata. However, after thorough examination, it was observed to be a cryptic species of T. circummaculata, and was described base.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026343
- DOI:
- 10.4238/gmr.15026343
Systematic tracking of altered modules identifies disrupted pathways in teratozoospermia
Authors: Z.Q. Huang, G.X. Wang, X.L. Jiang, E.P. Tian, W.L. Yao and T. Zeng
The objective of this study was to identify disrupted pathways in teratozoospermia by systematically tracking dysregulated modules in reweighted protein-protein interaction (PPI) networks. We inferred and reweighted the PPI networks of normal and teratozoospermia groups based on Spearman correlation coefficients. Modules in th.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027514
- DOI:
- 10.4238/gmr.15027514
MicroRNA-199a-3p is downregulated in gastric carcinomas and modulates cell proliferation
Authors: W. Peng, Z.-Y. Chen, L. Wang, Z. Wang and J. Li
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the translation of targeted mRNAs. An increasing amount of evidence indicates that miRNAs play important role in cancer pathogenesis, apoptosis, proliferation, and differentiation as oncogenes or tumor suppressors. Recently, miRNA-199a has been shown to be involved in many human cancers, although .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.20.5
- DOI:
- 10.4238/2013.August.20.5
Temporal variations of Hsp60 and HSF-1 in primary rat myocardial cells in vitro under heat stress
Authors: R. Buriro, Y.J. Lv, I. Ali, S. Tang, Z.J. Liu, M. Zhang, A. Adem, J. Hartung and E.D. Bao
The mechanisms involved in sudden animal death due to acute heart failure during heat stress are not well understood. We examined the relationship between heat stress-induced variations of protective Hsp60 and expression of its regulatory factor, HSF-1, in heat-stressed primary myocardial cells of neonatal rats in vitro through cardiac enzyme detection, i.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.20.2
- DOI:
- 10.4238/2013.August.20.2
Molecular cloning, functional verification, and evolution of TmPm3, the powdery mildew resistance gene of Triticum monococcum L.
Authors: C.Z. Zhao, Y.H. Li, H.T. Dong, M.M. Geng, W.H. Liu, F. Li, Z.F. Ni, X.J. Wang, C.J. Xie and Q.X. Sun
Powdery mildew (Pm) is one of the most harmful diseases in wheat. Three Pm-resistance genes, Pm3, Pm21, and Pm8, have been cloned but most Pm3/Pm8 alleles have lost their resistance to Pm in hexaploid wheat. In this study, a new Pm3 homolog gene (TmPm3) was isolated from Triticum monococcum L. using a homology-based cloning st.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028056
- DOI:
- 10.4238/gmr.15028056
Agrobacterium-mediated transformation of the �²-subunit gene in 7S globulin protein in soybean using RNAi technology
Authors: J. Qu, S.Y. Liu, P.W. Wang, S.Y. Guan, Y.G. Fan, D. Yao, L. Zhang and J.L. Dai
The objective of this study was to use RNA interference (RNAi) to improve protein quality and decrease anti-nutritional effects in soybean. Agrobacterium tumefaciens-mediated transformation was conducted using RNAi and an expression vector containing the 7S globulin β-subunit gene. The BAR gene was used as the selective m.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027561
- DOI:
- 10.4238/gmr.15027561
Characterization of a novel Cry8Ea3-binding V-ATPase Subunit A in Holotrichia parallela
Authors: W. Wei, G. Wei, Z. Dan1, Y. Xiaoping and Z. Yakun
Several receptor proteins of Cry toxin have been previously identified, including cadherin-like, aminopeptidase N, and alkaline phosphatase. In the present work, a novel binding protein, V-ATPase subunit A (HpVAA), was identified in Holotricia parallela larvae and characterized. We performed reverse transcription-polymerase ch.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038994
- DOI:
- 10.4238/gmr.15038994
Mutagenic influences of colchicine on phenological and molecular diversity of Calendula officinalis L.
Authors: Y.I. El-Nashar and M.H. Ammar
Six different colchicine concentrations: 0, 400, 800, 1200, 1600, and 2000 ppm, in combination with four soaking time treatments (1, 2, 3, and 4 h), were selected to assess the effects on germination, vegetative growth, and flower yield components in calendula plants. The molecular diversity among the treatments was assessed u.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027745
- DOI:
- 10.4238/gmr.15027745