Microbial Genetics
Evaluation of cellulolytic activity in insect digestive fluids
Authors: L.-J. Su, H.-F. Zhang, X.-M. Yin, M. Chen, F.-Q. Wang, H. Xie, G.-Z. Zhang and A.-D. Song
Efficient and low-cost cellulolytic enzymes are urgently needed to degrade recalcitrant plant biomass during the industrial production of lignocellulosic biofuels. Here, the cellulolytic activities in the gut fluids of 54 insect species that belong to 7 different taxonomic orders were determined using 2 different substrates, carboxymethyl cellulose (CMC) .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.11
- DOI:
- 10.4238/2013.January.4.11
Optimization of virus-induced gene silencing in pepper (Capsicum annuum L.)
Authors: J.-E. Wang, D.-W. Li, Z.-H. Gong and Y.-L. Zhang
Virus-induced gene silencing is currently a powerful tool for the study of gene function in plants. Here, we optimized the protocol for virus-induced gene silencing, and investigated factors that affect the efficiency of tobacco rattle virus-induced gene silencing in pepper plants. Consequently, an optimal protocol was obtained by the syringe-infiltration.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.24.4
- DOI:
- 10.4238/2013.July.24.4
Quantitative determination of activated coagulation factor XI as an impurity in therapeutic immunoglobulins from Chinese blood fractionation companies
Authors: L. Ma, P. Sun, F.Z. Lin, G. Diao and C.Q. Li
Residual activated coagulation factor XI (FXIa) has been suggested to play an important role in thromboembolic events associated with the use of intravenous immunoglobulin (IVIG) lots. This study investigated the predominant plasma proteases in 42 IVIG lots from 4 Chinese manufacturers. In one-stage clotting assays, the procoagulant activities of factors .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.17
- DOI:
- 10.4238/2013.January.4.17
Mitotic and meiotic behavior of rye chromosomes in wheat - Psathyrostachys huashanica amphiploid x triticale progeny
Authors: Q. Xie, H. Kang, D.L. Sparkes, S. Tao, X.M. Fan, L. Xu, X. Fan, L. Sha, H. Zhang, Y. Wang, J. Zeng and Y. Zhou
The dynamics of rye chromosomes during mitosis and meiosis was analyzed in a subset comprising 33 F3 lines from the cross of wheat, Psathyrostachys huashanica amphiploid (AABBDDNsNs) and hexaploid triticale (AABBRR), as visualized by genomic in situ hybridization. The results indicated that 31 of the total lines contained 4-14 rye chromosomes. Twenty-eigh.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.January.4.16
- DOI:
- 10.4238/2013.January.4.16
Molecular phylogeny of Threskiornithidae (Aves: Pelecaniformes) based on nuclear and mitochondrial DNA
Authors: J.L. Ramirez, C.Y. Miyaki and S.N. Del Lama
The family Threskiornithidae includes 13 genera and 32 species, and it is traditionally divided into 2 subfamilies: Plataleinae and Threskiornithinae. We present a phylogenetic reconstruction to test the monophyly of currently accepted subfamilies, including 15 species from both subfamilies and 10 genera of family Threskiornithidae. Phylogenetic trees wer.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.11
- DOI:
- 10.4238/2013.July.30.11
Assessment of toxic, genotoxic, antigenotoxic, and recombinogenic activities of Hymenaea courbaril (Fabaceae) in Drosophila melanogaster and mice
Authors: C.R. Vale, C.R. Silva, C.M.A. Oliveira, A.L. Silva, S. Carvalho and L. Chen-Chen
Hymenaea courbaril L., popularly known as jatobá, is a plant species that grows in the forests of South America. The species has been used for culinary purposes and in folk medicine to treat arthritis and inflammations. Due to the increasing use of this plant globally, the present study aimed to evaluate the toxic, genotoxic, recombinogenic, and an.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.9
- DOI:
- 10.4238/2013.July.30.9
Single nucleotide polymorphisms in immunity-related genes and their association with mastitis in Chilean dairy cattle
Authors: A.M. Carvajal, P. Huircan and A. Lepori
Mastitis remains a major cattle disease with great global economic implications. Various approaches are currently employed in attempts to improve understanding of mastitis resistance and develop phenotypic markers for use in breeding programs (e.g., somatic cell score), including QTL discovery, wide-genome association studies, and identification of candid.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.8
- DOI:
- 10.4238/2013.July.30.8
Inheritance of cytosine methylation patterns in purebred versus hybrid chicken lines
Authors: Q. Xu, D.X. Sun, J.L. Li, R. Liu, Y.C. Wang and Y. Zhang
We used methylation-sensitive amplified polymorphism to examine DNA methylation levels and CCGG patterns in parents and offsprings of 3 groups of adult chickens, purebred White Leghorn (AA), White Plymouth Rock (EE), and crossbred individuals (EA) using 10 primer combinations. We found that about 66% of the cytosines at CCGG sites were not methylated. Ful.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.6
- DOI:
- 10.4238/2013.July.30.6
Variation of the OsGI intron and its phenotypic associations in Oryza rufipogon Griff. and Oryza sativa L.
Authors: Y. Dong, Z. Chen, X. Pei, F. Wang, Q. Yuan, H. Wu, S. Jia and Y. Peng
We analyzed intron 9 of the OsGI gene in Oryza rufipogon and Oryza sativa in order to investigate evolutionary relationships in rice and the relationship between intron variation and phenotype. OsGI-9 was cloned in 38 O. rufipogon populations and in 139 O. sativa cultivars and the phylogeny was reconstructed. Seed cold tolerance and dormancy were quantifi.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.4
- DOI:
- 10.4238/2013.July.30.4
Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda
Authors: S.W. Jia, P. Liu, J. Li, J.T. Li and L.Q. Pan
The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.August.8.1
- DOI:
- 10.4238/2013.August.8.1