Microbial Genetics
A protocol for high-quality genomic DNA extraction from legumes
Authors: I.O. Agbagwa S. Datta P.G. Patil P. Singh N. Nadarajan
Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding li.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.14.1
- DOI:
- 10.4238/2012.September.14.1
Characterization of hemocyanin from the mud crab Scylla paramamosain and its expression analysis in different tissues, at various stages, and under Vibrio parahaemolyticus infection
Authors: J. Wang, F.Y. Zhang, W. Song, Y.B. Fang, J.H. Hu, M. Zhao, K.J. Jiang and L.B. Ma
Hemocyanin is an important respiratory protein in many arthropod and mollusk species. Here, four cDNAs (SpHc1, SpHc2, SpHc3, and SpHc4), encoding distinct hemocyanin subunits from Scylla paramamosain were cloned using EST analyses and the rapid amplification of cDNA ends. The four full-length cDNA fragments (SpHc1-4) were 2281, 2002, 2184, and 2069 bp, re.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Abundance and diversity of sulfate-reducing bacteria in the sediment of the Zhou Cun drinking water reservoir in Eastern China
Authors: X. Yang, T.L. Huang, L. Guo, C. Xia, H.H. Zhang and S.L. Zhou
Sulfate-reducing bacteria (SRB) play an important role in the sediments of bay areas, estuaries, and lakes. However, information regarding the genetic diversity of SRB in the sediments of drinking water reservoirs is scarce. In this study, we collected sediment samples from different sites in the Zhou Cun drinking water reservoir between April and June 20.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.15
- DOI:
- 10.4238/2015.May.29.15
Predicting bacterial essential genes using only sequence composition information
Authors: L.W. Ning, H. Lin, H. Ding, J. Huang, N. Rao and F.B. Guo
Essential genes are those genes that are needed by organisms at any time and under any conditions. It is very important for us to identify essential genes from bacterial genomes because of their vital role in synthetic biology and biomedical practices. In this paper, we developed a support vector machine (SVM)-based method to .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.17.8
- DOI:
- http://dx.doi.org/10.4238/2014.June.17.8
Activation of the ERK1/2 pathway by the CaMEK gene via adeno-associated virus serotype 9 in cardiomyocytes
Authors: Y.-N. Yang, W.-N. Ji1, Y.-T. Ma, X.-M. Li, B.-D. Chen, Y. Xiang and F. Liu
Extracellular signal-regulated kinase (ERK1/2) is one of the mitogen-activated protein kinases, key components of the reperfusion injury salvage kinase pathway, which plays an important role in protecting the myocardium from lethal ischemia-reperfusion injury. Constitutive activation of the mitogen-activated protein kinase kinase 1 (CaMEK) can promote ERK.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.17.1
- DOI:
- 10.4238/2012.October.17.1
Polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae)
Authors: H. Li, Z.-S. Zhou, J. Guo and L.H. Lin
We isolated and characterizated 12 polymorphic microsatellite loci in the rapid racerunner Eremias velox (Squamata: Lacertidae). The loci were screened in 37 E. velox individuals. The number of alleles ranged from 6 to 16. The observed heterozygosity ranged from 0.432 to 0.919, and the expected heterozygosity ranged from 0.685 to 0.902. These microsatelli.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.9.5
- DOI:
- 10.4238/2012.October.9.5
Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense
Authors: W.F. Wang, L.M. Mu, X.S. Wu, H. Yang and Q.J. Ning
Imidazole derivative KK-42 is a well-known regulator of insect growth. KK-42 pretreatment has been shown to promote the survival of Macrobrachium nipponense infected with Aeromonas hydrophila, possibly via activation of superoxide dismutase (SOD). In this study, the cytMnSOD gene was cloned from the hepatopancreas of M. nippon.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038484
- DOI:
- 10.4238/gmr.15038484
Validation of two real-time PCRs targeting the PE-PGRS 20 gene and the region of difference 4 for the characterization of Mycobacterium bovis isolates
Authors: M.L. Sales, A.A. Fonseca Jr., L. Orzil, A.P. Alencar, M.A. Hodon, M.A. Issa, P.M. Soares Filho, M.R. Silva, A.P. Lage and M.B. Heinemann
This study aimed to develop and validate real-time PCR for the diagnosis of Mycobacterium bovis isolates. Two hundred and seventy-four M. bovis isolates and 156 M. tuberculosis isolates were tested. Both qPCRs amplified all of the 274 M. bovis samples, but none of the 156 M. tuberculosis samples. The qPCR for PE-PGRS 20 had 91.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.18.3
- DOI:
- http://dx.doi.org/10.4238/2014.June.18.3
Effect of mutations in a simian virus 40 PolyA signal enhancer on green fluorescent protein reporter gene expression
Authors: H.G. Wang, X.F. Wang, X.Y. Jing, Z. Li, Y. Zhang and Z.J. Lv
Our previous studies have shown that tandem Alu repeats inhibit green fluorescent protein (GFP) gene expression when inserted downstream of the GFP gene in the pEGFP-C1 vector. We found that the 22R sequence (5'-GTGAAAAAAATGCTTTATTTGT-3') from the antisense PolyA (240 bp polyadenylation signal) of simian virus 40, eliminated r.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1169
- DOI:
- 10.4238/vol10-3gmr1169
Molecular identification of phosphate-solubilizing native bacteria isolated from the rhizosphere of Prosopis glandulosa in Mexicali valley
Authors: L. Moreno-RamÃ?Ârez, D. GonzÃ?¡lez-Mendoza, C. Cecena-Duran and O. Grimaldo-Juarez
One of the main limitations in intensive crop production in Northwestern Mexico is the dependence on the use of phosphate fertilizer. In this study, we isolated indigenous microorganisms with phosphate solubilization capacities from mesquite (Prosopis glandulosa) present in the Mexicali valley. In total, 4 bacteria were isolated from the rhizosphere of me.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.9
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.9