Microbial Genetics
Application of RNA-seq to reveal the transcript profile in bacteria
Authors: A.C. Pinto, H.P. Melo-Barbosa, A. Miyoshi, A. Silva and V. Azevedo
The large number of microbial genomes deposited in databanks has opened the door for in-depth studies of organisms, including post-genomics investigations. Thanks to new generation sequencing technology, these studies have made advances that have lead to extraordinary discoveries in bacterial transcriptomics. In this review, w.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1554
- DOI:
- 10.4238/vol10-3gmr1554
Genotoxic effects of rotenone on cultured lymphocytes
Authors: PatrÃ?Âcia Danielle Lima de Lima, Elizabeth Sumi Yamada, Edmar Tavares da Costa, Claudia do O. Pessoa, Silvia Helena Barem Rabenhorst, Marcelo de Oliveira Bahia, PlÃ?Ânio Cerqueira Cardoso, Raquel Alves Santos, Marilia de Arruda Cardoso Smith and Rommel RodrÃ?Âguez Burbano
Rotenone is a heterocyclic compound widely used as an insecticide, acaricide and piscicide. Its toxicity is mainly caused by the inhibition of mitochondrial respiratory processes and ATP production, resulting in the generation of reactive oxygen species. Reactive oxygen species can interact with DNA, RNA and proteins, leading .. Read More»
- Genet. Mol. Res. 4(4):
Two chitin metabolic enzyme genes from Hyriopsis cumingii: cloning, characterization, and potential functions
Authors: G.-L. Wang B. Xu Z.-Y. Bai J.-L. Li
Chitin, the second most important natural polymer in the world, and its N-deacetylated derivative chitosan are found in a wide variety of organisms. These versatile biopolymers are associated with a broad range of biological functions. This article is the first to report the potential functions of 2 chitin metabolic enzyme gen.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.15.4
- DOI:
- 10.4238/2012.October.15.4
Genetic diversity of ITS sequences of Bursaphelenchus xylophilus
Authors: J.M.S. Cardoso L. Fonseca I. Abrantes
The sequence variation of internal transcribed spacer (ITS) regions of ribosomal DNA has been routinely used for species identification and species-level phylogeny of the pinewood nematode, Bursaphelenchus xylophilus. In this study, the intraspecies ITS genetic diversity of B. xylophilus was evaluated. Three pinewood nematode isolates from the United Stat.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.15.1
- DOI:
- 10.4238/2012.October.15.1
Development and characterization of microsatellite markers for molecular genetic diversity in Atrina pectinata
Authors: Y.F. Ning, Z.B. Li, J.B. Shangguan, Y.S. Huang, P.D. Jiang, A.L. Xu and Y. Yuan
The pen shell, Atrina pectinata, is an economically valuable species that is widely distributed along the coastal waters of temperate and tropical areas, mainly growing in the Indian and Pacific Oceans. Eight novel microsatellite loci from the genome of A. pectinata were developed using fast isolation by amplified fragment length polymorphism of sequence .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.17
- DOI:
- 10.4238/2015.July.27.17
Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae)
Authors: R.O. Manoel, M.L.M. Freitas, E.V. Tambarussi, J. Cambuim, M.L.T. Moraes and A.M. Sebbenn
Genipa americana is a tropical tree species that is widely distributed in the humid tropical and subtropical regions of Central and South America. This study investigated Mendelian inheritance, genetic linkage, and genotypic disequilibrium at six microsatellite loci developed for G. americana. Adult trees (188) and regenerants (163) were sampled and genot.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.4
- DOI:
- 10.4238/2015.July.27.4
Identification of Pseudomonas syringae pv. actinidiae strains causing bacterial canker of kiwifruit in the Anhui Province of China, and determination of their streptomycin sensitivities
Authors: X. Yang, X.-K. Yi, Y. Chen, A.-F. Zhang, J.-Y. Zhang, Z.-H. Gao, Y.-J. Qi and Y.-L. Xu
27Bacterial canker, caused by Pseudomonas syringae pv. actinidiae, is one of the most severe diseases of kiwifruit. It has become an international pandemic and threatens the sustainable development of kiwifruit production in all main kiwi-growing regions worldwide. Streptomycin has been the major bactericide for the control of kiwifruit canker, especially.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.7
- DOI:
- 10.4238/2015.July.27.7
Transferability and characterization of simple sequence repeat markers from Anacardium occidentale to A. humile (Anacardiaceae)
Authors: L.G. Cota P.A. Moreira E.V. Menezes A.S. Gomes A.R.O. Ericsson D.A. Oliveira A.F. Melo Jr.
Use of molecular markers can be limited by the high cost and extensive time required for their development. Transfer of simple sequence repeat (SSR) markers reduces the cost and time limitations and has allowed the use of these markers in a larger number of species. We tested 11 SSR markers previously developed for Anacardium occidentale on A. humile. The.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.17.7
- DOI:
- 10.4238/2012.October.17.7
Relationship between polymorphisms in exon 10 of FSHR gene and litter size in swine
Authors: X.D. Zhang, H.Y. Zhu, J. Zhou, N. Wang, N. Zhou, L. Huang, T. Wu, Y.F. Feng, Y.Y. Ding and Z.J. Yin
Follicle-stimulating hormone (FSH), a glycoprotein secreted by the anterior pituitary, can regulate ovarian function through the FSH receptor (FSHR). To evaluate the effects of the FSHR gene on reproductive traits in pigs, polymorphisms in exon 10 of the FSHR gene were observed by polymerase chain reaction-single-strand conformation polymorphism, and 3 si.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.13
- DOI:
- 10.4238/2015.July.27.13
Investigation of the role of VEGF gene polymorphisms in the risk of osteosarcoma
Authors: G. Zhang, R. Bai, T. Zhang, H. Zhang, S.Z. Wen and D.M. Jiang
We conducted a case-control study in a Chinese population, and assessed whether the VEGF -634G/C, +936C/T, and +1612G/A polymorphisms could affect the risk of osteosarcoma and its association with environmental factors. A total of 180 consecutive osteosarcoma patients and 360 controls were included in our study. The genotypes of VEGF -634G/C, +936C/T, and.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.27.16
- DOI:
- 10.4238/2015.July.27.16