Microbial Genetics

Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)

Authors: R.C.C. Vasconcellos, T.F.C. Lima, C.N. Fernandes-Brum, A. Chalfun-Junior and J.B. Santos

The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses. This study aimed to i) investigate variation in the expression of different common bean (Phaseolus vulgaris L.) genotypes and its re.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038269

DOI:

10.4238/gmr.15038269

Characterization and heterologous expression of isocitrate lyase of Paracoccidioides brasiliensis

Authors: A.H.S. Cruz

Genet. Mol. Res. 11(4):

2012.December.17.5

DOI:

10.4238/2012.December.17.5

Phylogenetic analysis reveals gene conversions in multigene families of rhizobia

Authors: J.E. Hernandez-Salmeron and G. Santoyo

Gene families are an important and intrinsic trait of rhizobial species. These gene copies can participate in non-reciprocal recombination events, also called gene conversions. Gene conversion has diverse roles, but it is usually implicated in the evolution of multigene families. Here, we searched for gene conversions in multi.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1118

DOI:

10.4238/vol10-3gmr1118

Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance

Authors: S. Ghanem

In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E. coli DH5a ΔrecA (recombi.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1334

DOI:

10.4238/vol10-3gmr1334

Phylogeny and systematics of Indian Polygonum sensu lato in the subfamily Polygonoideae based on ITS sequences of nuclear ribosomal DNA

Authors: R.K. Choudhary S.-H. Park J. Lee

The nuclear ribosomal DNA internal transcribed spacer (ITS) sequences from 44 Indian Polygonum taxa were examined to investigate relationships among various sections proposed by earlier researchers. The maximum parsimony trees obtained from analysis of the ITS sequences suggested eight major groups of the Indian Polygonum spp. The relationships among diff.. Read More»

Genet. Mol. Res. 11(4):

2012.October.4.1

DOI:

10.4238/2012.October.4.1

Effect of domestication on microorganism diversity and anaerobic digestion of food waste

Authors: S.J. Bi, X.J. Hong, G.X. Wang, Y. Li, Y.M. Gao, L. Yan, Y.J. Wang and W.D. Wang

To accomplish the rapid start-up and stable operation of biogas digesters, an efficient inoculum is required. To obtain such an inoculum for food waste anaerobic digestion, we domesticated dairy manure anaerobic digestion residue by adding food waste every day. After 36 days, the pH and biogas yield stabilized signifying the completion of domestication. D.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038417

DOI:

10.4238/gmr.15038417

Isolation and characterization of 48 polymorphic microsatellite markers for the blood clam Scapharca broughtonii (Arcidae)

Authors: J.-T. Tian Z.-H. Liu L.-Q. Zhou B. Wu P. Liu A.-G. Yang

Blood clams (Scapharca broughtonii) are widely cultivated and consumed in noutheast Asia. Forty-eight polymorphic microsatellite loci were developed for this clam using magnetic-bead hybridization enrichment. The number of alleles per locus ranged from 2 to 14. Polymorphism of these loci was assessed in 30 individuals from a population collected from coas.. Read More»

Genet. Mol. Res. 11(4):

2012.October.11.2

DOI:

10.4238/2012.October.11.2

Polymorphism in Metarhizium anisopliae var. anisopliae (Hypocreales: Clavicipitaceae) based on internal transcribed spacer-RFLP, ISSR and intron markers

Authors: P.V. Tiago, M.P. Carneiro-Le�£o, M.L.A. Lima, N.T. Oliveira and E.A. Luna-Alves Lima

Isolates of entomopathogenic fungus Metarhizium anisopliae var. anisopliae were characterized using internal transcribed spacer-RFLP, ISSR and intron splice site primers. Thirty-seven isolates were studied, most of which were obtained from the sugar cane pest, Mahanarva fimbriolata (Hemiptera: Cercopidae) from Tangará d.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1069

DOI:

10.4238/vol10-3gmr1069

Identification of genes encoding hypothetical proteins in open-reading frame expressed sequence tags from mammalian stages of Trypanosoma cruzi.

Authors: C. Martins, J.L. Reis-Cunha, M.N. Silva, E.G. Pereira, G.J. Pappas Jr., D.C. Bartholomeu and B. Zingales

Approximately 50% of the predicted protein-coding genes of the Trypanosoma cruzi CL Brener strain are annotated as hypothetical or conserved hypothetical proteins. To further characterize these genes, we generated 1161 open-reading frame expressed sequence tags (ORESTES) from the mammalian stages of the VL10 human strain. Sequ.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1140

DOI:

10.4238/vol10-3gmr1140

Cytogenetic and genotoxic effects of the insecticides, imidacloprid and methamidophos

Authors: N. Ulku Karabay and M. Gunnehir Oguz

We examined the cytogenetic and genotoxic effects of the neonicotinoid insecticide imidacloprid and the organophosphate insecticide methamidophos, when administered alone or in combination. These insecticides were tested with the bone marrow chromosome aberration assay and micronucleus test in rats and by the bacterial mutatio.. Read More»

Genet. Mol. Res. 4(4):