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Microbial Genetics   Research Article

Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle

Authors: X.E. Shi, Z.Y. Song, Q.M. Yang, Y.G. Liu and G.S. Yang

We examined the expression of myosin heavy chain (MyHC) isoforms and forkhead box transcription factor O1 (FoxO1) in porcine soleus and extensor digitorum longus (EDL) muscles to clarify the correlation of FoxO1 and the relative abundance of transcripts of MyHC isoforms. Soleus muscle was found to be redder than EDL muscles in pigs, and immunohistochemica.. Read More»

Genet. Mol. Res. 13(4):
2014.December.4.18
DOI:
10.4238/2014.December.4.18
Microbial Genetics   Research Article

Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain

Authors: J.H. Hu1,2*, F.Y. Zhang1*, K.J. Jiang1, Y.B. Fang1,2, J. Wang1,2, M. Zhao1,2, Z.G. Qiao1 and L.B. Ma1

The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx.. Read More»

Genet. Mol. Res. 13(4):
2014.December.4.19
DOI:
10.4238/2014.December.4.19
Microbial Genetics   Research Article

Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations

Authors: J. Chen, S.F. Fang, D.H. Zhou, Z.Y. Li, G.H. Liu and X.Q. Zhu

Toxoplasma gondii is an opportunistic protozoan parasite that infects a wide range of animals, including humans. The T. gondii eukaryotic translation initiation factor 4A (eIF4A) protein is expressed in the tachyzoite, but its expression is markedly downregulated in the bradyzoite, and it is therefore considered to be associat.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.April.29.14
DOI:
http://dx.doi.org/10.4238/2014.April.29.14
Microbial Genetics   Research Article

Efficient production of transgenic melon via Agrobacterium-mediated transformation

Authors: I. Bezirganoglu, S.Y. Hwang, J.F. Shaw and T.J. Fang

Oriental melon (Cucumis melo L. var. makuwa) is an important fruit for human consumption. However, this plant species is one of the most recalcitrant to genetic transformation. The lack of an efficient in vitro system limits the development of a reproducible genetic transformation protocol for Oriental melon. In this study, an.. Read More»

Genet. Mol. Res. 13(2):
2014.April.25.7
DOI:
10.4238/2014.April.25.7
Microbial Genetics   Research Article

Confirmation of soybean plastid rRNAs by formaldehyde denaturing agarose gel electrophoresis

Authors: Y.Q. Zhu1,2, Y. Zheng1,2, H.B. Chen1,2 and L.Q. Huang1,2

Owing to their prokaryotic origin, plastid rRNAs are mainly 23s/16s/5s rRNAs. We present a novel plant RNA isolation method in this paper. Also, not only the eukaryotic 28s (26s, 25s)/18s rRNAs but the prokaryotic 26s/23s rRNAs as well were demonstrated in a single sample for the first time by formaldehyde denaturing agarose gel electrophoresis.. Read More»

Genet. Mol. Res. 13(4):
Microbial Genetics   Research Article

Genetic variability and phylogenetic aspects in species of the genus Macrobrachium

Authors: A.L. Guerra, A.V.B. Lima, R.V. Lucato Júnior, M.C. Chiachio, F.G. Taddei and L. Castiglioni

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this .. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.9.7
DOI:
http://dx.doi.org/10.4238/2014.May.9.7
Microbial Genetics   Research Article

Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes

Authors: C.R. Rainho, S.M. Corrêa, J.L. Mazzei, C.A.F. Aiub and I. Felzenszwalb

The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sen.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.9.9
DOI:
http://dx.doi.org/10.4238/2014.May.9.9
Microbial Genetics   Research Article

Molecular authentication of multi-species honeysuckle tablets

Authors: C. Jiang*, Y. Yuan*, M. Chen and L. Huang

Authenticating multi-species original raw materials in commercial formulations is difficult. Jin Yin Hua and Shan Yin Hua, both classified as raw honeysuckle materials in the Chinese Pharmacopoeia, are used in various medicines. Differentiating one variety from another is difficult based on chemical analysis. We developed molecular authentication of multi.. Read More»

Genet. Mol. Res. 12(4):
2013.October.22.2
DOI:
10.4238/2013.October.22.2
Microbial Genetics   Research Article

Expression of neurotrophin-4/5 in Streptococcus pneumoniae meningitis

Authors: H. Zhang, H.Y. Qian and L. Li

This study aims to investigate the expression of neurotrophin-4/5 (NT-4/5) in the pallium and hippocampus in juvenile rats with intraventricular injection of Streptococcus pneumoniae. We used 40 SPF SD rats (3 weeks old, regardless of gender) in this study. We drew 50 μL cerebrospinal fluid, and then, we injected 50 μL n.. Read More»

Genet. Mol. Res. 13(2):
2014.April.16.8
DOI:
10.4238/2014.April.16.8
Microbial Genetics   Research Article

Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori

Authors: Q.M. Chen, D.J. Cheng, S.P. Liu, Z.G. Ma, X. Tan and P. Zhao

Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven gene.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.13.2
DOI:
http://dx.doi.org/10.4238/2014.May.13.2