Microbial Genetics
Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain
Authors: J.H. Hu1,2*, F.Y. Zhang1*, K.J. Jiang1, Y.B. Fang1,2, J. Wang1,2, M. Zhao1,2, Z.G. Qiao1 and L.B. Ma1
The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.19
- DOI:
- 10.4238/2014.December.4.19
Sequence variation in the Toxoplasma gondii eIF4A gene among strains from different hosts and geographical locations
Authors: J. Chen, S.F. Fang, D.H. Zhou, Z.Y. Li, G.H. Liu and X.Q. Zhu
Toxoplasma gondii is an opportunistic protozoan parasite that infects a wide range of animals, including humans. The T. gondii eukaryotic translation initiation factor 4A (eIF4A) protein is expressed in the tachyzoite, but its expression is markedly downregulated in the bradyzoite, and it is therefore considered to be associat.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.14
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.14
Efficient production of transgenic melon via Agrobacterium-mediated transformation
Authors: I. Bezirganoglu, S.Y. Hwang, J.F. Shaw and T.J. Fang
Oriental melon (Cucumis melo L. var. makuwa) is an important fruit for human consumption. However, this plant species is one of the most recalcitrant to genetic transformation. The lack of an efficient in vitro system limits the development of a reproducible genetic transformation protocol for Oriental melon. In this study, an.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.25.7
- DOI:
- 10.4238/2014.April.25.7
Confirmation of soybean plastid rRNAs by formaldehyde denaturing agarose gel electrophoresis
Authors: Y.Q. Zhu1,2, Y. Zheng1,2, H.B. Chen1,2 and L.Q. Huang1,2
Owing to their prokaryotic origin, plastid rRNAs are mainly 23s/16s/5s rRNAs. We present a novel plant RNA isolation method in this paper. Also, not only the eukaryotic 28s (26s, 25s)/18s rRNAs but the prokaryotic 26s/23s rRNAs as well were demonstrated in a single sample for the first time by formaldehyde denaturing agarose gel electrophoresis.. Read More»
- Genet. Mol. Res. 13(4):
Genetic variability and phylogenetic aspects in species of the genus Macrobrachium
Authors: A.L. Guerra, A.V.B. Lima, R.V. Lucato J�?ºnior, M.C. Chiachio, F.G. Taddei and L. Castiglioni
The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this .. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.7
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.7
Comparison of the sensitivity of strains of Salmonella enterica serovar Typhimurium in the detection of mutagenicity induced by nitroarenes
Authors: C.R. Rainho, S.M. Corr�?ªa, J.L. Mazzei, C.A.F. Aiub and I. Felzenszwalb
The use of strains of Salmonella enterica serovar Typhimurium with different metabolic capacities can indicate the class or classes of compounds present in an environmental sample and enable the diagnosis of the mutagenic activity of these pollutants adsorbed on particulate matter (PM) in the air. In the present study, the sen.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.9
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.9
Molecular authentication of multi-species honeysuckle tablets
Authors: C. Jiang*, Y. Yuan*, M. Chen and L. Huang
Authenticating multi-species original raw materials in commercial formulations is difficult. Jin Yin Hua and Shan Yin Hua, both classified as raw honeysuckle materials in the Chinese Pharmacopoeia, are used in various medicines. Differentiating one variety from another is difficult based on chemical analysis. We developed molecular authentication of multi.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.2
- DOI:
- 10.4238/2013.October.22.2
Expression of neurotrophin-4/5 in Streptococcus pneumoniae meningitis
Authors: H. Zhang, H.Y. Qian and L. Li
This study aims to investigate the expression of neurotrophin-4/5 (NT-4/5) in the pallium and hippocampus in juvenile rats with intraventricular injection of Streptococcus pneumoniae. We used 40 SPF SD rats (3 weeks old, regardless of gender) in this study. We drew 50 μL cerebrospinal fluid, and then, we injected 50 μL n.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.8
- DOI:
- 10.4238/2014.April.16.8
Genome-wide identification and expression profiling of the fatty acid desaturase gene family in the silkworm, Bombyx mori
Authors: Q.M. Chen, D.J. Cheng, S.P. Liu, Z.G. Ma, X. Tan and P. Zhao
Fatty acid desaturases exist in all living organisms and play important roles in many different biologic processes, such as fatty acid metabolism, lipid biosynthetic processes, and pheromone biosynthetic processes. Using the available silkworm genome sequence, we identified 14 candidate fatty acid desaturase genes. Eleven gene.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.13.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.13.2
Complete mitochondrial genomes of the Bright Sunbeam Curetis bulis and the Small Copper Lycaena phlaeas (Lepidoptera: Lycaenidae) and their phylogenetic implications
Authors: L.L. Zhang1, J.S. Hao1,2, D.Y. Huang1, X.Y. Sun2, J.J. Hao1, C.M. Peng1 and Q. Yang2
In this study, the complete mitochondrial genomes of Curetis bulis and Lycaena phlaeas were determined and analyzed. The circular genomes are 15,162 bp long for C. bulis and 15,280 bp long for L. phlaeas, with a total A+T content of 82.6 and 83.1%, respectively. Both mitogenomes contain 37 genes, and their gene orders are similar to those of other lepidop.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.10.9
- DOI:
- 10.4238/2013.October.10.9