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Microbial Genetics   Research Article

Complete mitochondrial genomes of the Bright Sunbeam Curetis bulis and the Small Copper Lycaena phlaeas (Lepidoptera: Lycaenidae) and their phylogenetic implications

Authors: L.L. Zhang1, J.S. Hao1,2, D.Y. Huang1, X.Y. Sun2, J.J. Hao1, C.M. Peng1 and Q. Yang2

In this study, the complete mitochondrial genomes of Curetis bulis and Lycaena phlaeas were determined and analyzed. The circular genomes are 15,162 bp long for C. bulis and 15,280 bp long for L. phlaeas, with a total A+T content of 82.6 and 83.1%, respectively. Both mitogenomes contain 37 genes, and their gene orders are similar to those of other lepidop.. Read More»

Genet. Mol. Res. 12(4):
2013.October.10.9
DOI:
10.4238/2013.October.10.9
Microbial Genetics   Research Article

Bioprospection of bacteria and yeasts from Atlantic Rainforest soil capable of growing in crude-glycerol residues

Authors: E.A.A. Duarte1, G.V. Lacerda Jr1, T.A.S. de Oliveira2, M. Brendel1, L.L. Loguercio1, J.C. de M. Cascardo1†

The increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. This could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. We explored an Atlantic Rainforest soil sample to search for crude glycerol-degrading microorganisms. .. Read More»

Genet. Mol. Res. 12(4):
2013.October.10.8
DOI:
10.4238/2013.October.10.8
Microbial Genetics   Research Article

Multidrug-resistant genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in Acinetobacter baumannii strains

Authors: J.-T. Wen, Y. Zhou, L. Yang and Y. Xu

We examined the distribution of genes of aminoglycoside-modifying enzymes and 16S rRNA methylases in multidrug-resistant Acinetobacter baumannii to explore the association of these genes with drug resistance. Strains isolated from clinical specimens were screened using an automatic microbial identification system, and 9 aminog.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.16.9
DOI:
http://dx.doi.org/10.4238/2014.May.16.9
Microbial Genetics   Research Article

Molecular cloning and expression of the porcine S14R gene in Escherichia coli

Authors: Y.J. Guo1*, G.Z. Liu1*, C.M. Wang1, Y.Y. Wang2, H.J. Li2, K. Zhong2, W.F. Lu2, Y.L. Wang2, G.Y. Yang2

We amplified S14R protein gene cDNA of porcine, cloned it into a prokaryotic expression plasmid, and expressed it in Escherichia coli. A pair of primers was designed based on the cDNA sequence of the porcine S14R gene in GenBank. The target gene fragment from porcine liver tissue was amplified by RT-PCR. Confirmed by auto-sequencing, the target gene fragm.. Read More»

Genet. Mol. Res. 12(4):
2013.October.10.6
DOI:
10.4238/2013.October.10.6
Microbial Genetics   Research Article

Full-length cDNA cloning and structural characterization of preproinsulin in Alligator sinensis

Authors: R. Zhang, S.Z. Zhang, E. Li, C. Wang, C.L. Wang and X.B. Wu

Insulin is an important endocrine hormone that plays a critical physiological role in regulating metabolism and glucostasis in vertebrates. In this study, the complete cDNA of Alligator sinensis preproinsulin gene was cloned for the first time by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods; the amino acid s.. Read More»

Genet. Mol. Res. 13(4):
Microbial Genetics   Research Article

Microsporogenesis in Paspalum conspersum Schrad. (Virgata group) with different ploidy levels

Authors: L. Janke1, F.H.D. Souza2, J.F.M. Valls3 and M.S. Pagliarini1

Knowledge about the cytology and reproductive behavior of a species is indispensable for hybridization programs. This is especially true for species belonging to the genus Paspalum, among which apomixis and a wide range of ploidy levels are frequently found. Paspalum conspersum Schrad. is a robust and warm-season perennial bunchgrass native to South Ameri.. Read More»

Genet. Mol. Res. 12(4):
2013.October.24.6
DOI:
10.4238/2013.October.24.6
Microbial Genetics   Research Article

Comparative proteomic analysis between early developmental stages of the Coffea arabica fruits

Authors: G.B. Bandil1, R.M. Etto2, C.W. Galvão2, H.J.O. Ramos3, E.M. Souza4, F.O. Pedrosa4, D.F.S. Chaves4, L.F. Huergo4 and R.A. Ayub1

Coffee is one of the most valuable agricultural commodities. There is much agronomic research on coffee, but molecular knowledge of its fruit development and ripening is limited... Read More»

Genet. Mol. Res. 12(4):
2013.October.29.4
DOI:
10.4238/2013.October.29.4
Microbial Genetics   Research Article

Karyotype variation in cultivars and spontaneous cocoa mutants (Theobroma cacao L.)

Authors: G.S.F. Figueiredo, C.A.F. Melo, M.M. Souza, I.S. Araújo, H.A. Zaidan, J.L. Pires and D. Ahnert

Four mutant cocoa accessions with morphological changes and a cultivar sample were karyomorphologically characterized. Slides were prepared by enzymatic digestion of the root meristem and squashed in 45% acetic acid, followed by 2% Giemsa staining. The chromosome number of 2n = 20 was seen in all accessions. The karyotype formula for Cacau Comum and Cacau.. Read More»

Genet. Mol. Res. 12(4):
2013.October.18.5
DOI:
10.4238/2013.October.18.5
Microbial Genetics   Research Article

Molecular characterization by amplified ribosomal DNA restriction analysis and antimicrobial potential of endophytic fungi isolated from Luehea divaricata (Malvaceae) against plant pathogenic fungi and pathogenic bacteria

Authors: J. Bernardi-Wenzel1, A. Garcia2, J.L. Azevedo2 and J.A. Pamphile2

Luehea divaricata is an important plant in popular medicine; it is used for its depurative, anti-inflammatory, and other therapeutic activities. We evaluated the antimicrobial activity of endophytic fungi isolated from leaves of L. divaricata against phytopathogens and pathogenic bacteria, and characterized the isolates based on amplified ribosomal DNA re.. Read More»

Genet. Mol. Res. 12(4):
2013.October.29.2
DOI:
10.4238/2013.October.29.2
Microbial Genetics   Research Article

Analysis of GATA1 mutations and leukemogenesis in newborns with Down syndrome

Authors: L.B. Queiroz, B.D. Lima, J.F. Mazzeu, R. Camargo, M.S. Córdoba, I.Q. Magalhães, C. Martins-de-Sá and I. Ferrari

It has been reported that patients with Down syndrome (DS) frequently develop transient myeloproliferative disorder (TMD) and less commonly myeloid leukemia in DS (ML-DS). We examined the pathogenetic relationship of these conditions with somatic mutations of the GATA1 gene in children with both TMD and ML-DS. To determine the incidence of GATA1 mutations.. Read More»

Genet. Mol. Res. 12(4):
2013.October.18.1
DOI:
10.4238/2013.October.18.1