Microbial Genetics
Cloning and prokaryotic expression of the porcine lipasin gene..
Authors: M.M. Li* J. Geng, Y.J. Guo, X.Q. Jiao, W.F. Lu, H.S. Zhu, Y.Y. Wang and G.Y. Yang
Lipasin has recently been demonstrated to be involved in lipid metabolism. In this study, two specific primers were used to amplify the lipasin open reading frame from porcine liver tissue. The polymerase chain reaction product was cloned to a pGEM®-T Easy Vector, digested by SalI and NotI, and sequenced. The lipasin fragment was then cloned to a pET2.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.34
- DOI:
- 10.4238/2015.November.18.34
Gene expression profiles of the heterotrophic microalga Chlorella pyrenoidosa F-9
Authors: X. Sun1, N.J. Xu1, L.Z. Jiang1, W. Hu1, M.H. Fan1 and Z.M. Liu2
A strain of the microalga Chlorella pyrenoidosa F-9 in our laboratory showed special characteristics when transferred from autotrophic to heterotrophic culture. In order to elucidate the possible metabolic mechanism, the gene expression profiles of the autonomous organelles in the green alga C. pyrenoidosa under autotrophic and heterotrophic cultivation w.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.17
- DOI:
- 10.4238/2014.October.20.17
Cytoplasmic and extracellular expression of pharmaceutical-grade mycobacterial 65-kDa heat shock protein in Lactococcus lactis
Authors: M.S.P. de Azevedo, C.S. Rocha, N. Electo, D.S. Pontes, J.B. Molfetta, E.D.C. Gon�§alves, V. Azevedo, C.L. Silva and A. Miyoshi
Lactic acid bacteria (LAB) are an attractive and safe alternative for the expression of heterologous proteins, as they are nonpathogenic and endotoxin-free organisms. Lactococcus lactis, the LAB model organism, has been extensively employed in the biotechnology field for large-scale production of heterologous proteins, and its.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.14
- DOI:
- 10.4238/2012.April.27.14
Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance
Authors: M. Lu*, L. Wang*, J. Zhang, S. Sun, Y. Li, W. Du, J. Wu, J. Zhao, Q. Yang and X. Chen
A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.10
- DOI:
- 10.4238/2014.December.18.10
DNA methyltransferase 3B -149C/T polymorphism and the risk of laryngeal squamous cell carcinoma: a case-control study
Authors: X.M. Zhang, S. Li and Q.M. Zhang
A variety of molecular epidemiological studies have been conducted to examine the association between the DNMT3B -149C/T polymorphism and cancer susceptibility; however, there has been no study investigating the association between the DNMT3B -149C/T polymorphism and the risk of laryngeal squamous cell carcinoma (LSCC) until now. To determine the role of .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.6
- DOI:
- 10.4238/2015.October.21.6
Differential expression of COX-2 in osteoarthritis and rheumatoid arthritis
Authors: H.W. Fan, G.Y. Liu, C.F. Zhao, X.F. Li and X.Y. Yang
In this study, we investigated the differential expression profiles of cyclooxygenase-2 (COX-2) mRNA and proteins in osteoarthritis (OA) and rheumatoid arthritis (RA) patients to elucidate the role of COX-2 expression in the pathogenesis and development of these diseases and to provide novel drug targets for treating arthritis. A total of 60 patients who .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.7
- DOI:
- 10.4238/2015.October.21.7
Breeding of a target genotype variety based on identified chalkiness marker-QTL associations in rice (Oryza sativa L.)
Authors: X. Liu, Y.R. Du, X.H. Li, X.L. Li, W.Q. Yang and Y. Wang
The aim of this study was to breed a target genotype variety based on the identified chalkiness marker-QTL (quantitative trait locus) associations in rice. First, a permanent mapping population of rice that consisted of 525 recombinant inbred lines (RILs), which were derived from Zhenshan 97/Minghui 63, was used to identify QTLs with additive effects for .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.10
- DOI:
- 10.4238/2015.October.21.10
Hyperproliferative action of bovine papillomavirus: genetic and histopathological aspects
Authors: R.P. Araldi, T.C. Melo, A.C. Neves, D.D. Spadacci-Morena, R.F. Magnelli, D.G. Modolo, P.L. de-S�¡-J�ºnior, J. Mazucchelli-de-Souza, R.F. Carvalho, W. Be�§ak and R.C. Stocco
The bovine papillomavirus (BPV) causes papillomas that regress spontaneously, but can also progress to malignancy. This study evaluated the role of BPV in oncogenesis. Twenty-four samples from uninfected calves and the papillomas of BPV infected cattle were subjected to molecular diagnosis, as well as histopathological and immunohistochemical analyses. Th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.15
- DOI:
- 10.4238/2015.October.21.15
Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes
Authors: Y.H. Zhao, Z.B. Zhang, C.Q. Zhao, Y. Zhang, Y.F. Wang, W.J. Guan and Z.Q. Zhu
The genes of top athletes are a valuable genetic resource for the human race, and could be exploited to identify novel genes related to sports ability, as well as other functions. We analyzed the expressed sequence tags from top half-pipe snowboarding athletes using the SMART complementary DNA (cDNA) library construction method to elucidate the characteri.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.13
- DOI:
- 10.4238/2015.October.21.13
Matrilineage differentiation of the genus Tetragonisca using mitochondrial DNA markers and the polymerase chain reaction-restriction fragment length polymorphism technique
Authors: S.A. Santos, A.R. Bronzato, B.M.T. Moreira, K.F. Araujo, L. Ronqui, C.A. Mangolin, V.A.A. Toledo and M.C.C. Ruvolo-Takasusuki
The Meliponinae are important pollinators of plant species, and one of the most managed species is Tetragonisca angustula. Initially, two subspecies were identified in T. angustula: T. angustula angustula and T. angustula fiebrigi. Subsequently, T. a. fiebrigi was considered a species, based on the coloration of its mesepisternum. The objective of the pre.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.2
- DOI:
- 10.4238/2015.October.21.2