Abstract

Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance

Author(s): M. Lu*, L. Wang*, J. Zhang, S. Sun, Y. Li, W. Du, J. Wu, J. Zhao, Q. Yang and X. Chen

A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The mitotic and meiotic investigations showed that the chromosome number and configuration of 13-2-2 were 2n = 42 = 21 II. GISH using rye genomic DNA as a probe detected a pair of R genome chromosome arms with strong hybridization signals in 13-2-2.

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