Medical Genetics
A real-time RT-PCR assay for rapid detection of coxsackievirus A10
Authors: C.Y. Mu, A.Y. Wang, C. Chen, L. Zhao and Z. Li
Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) have been the primary causative agents of hand, foot, and mouth disease (HFMD) outbreaks in mainland China in the past. Hence, the surveillance of HFMD has mostly focused on these viruses. However, in recent years, coxsackievirus A10 (CA10) has also been associated with the increasing sporadic HFMD cases.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.21
- DOI:
- 10.4238/2015.December.21.21
N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats
Authors: Y. Zhou , Y. Liu, S.X. Yang and Z. Wang
This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.11
- DOI:
- 10.4238/2015.August.14.11
Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells
Authors: L. Zhang, Y.H. Xie and B.R. Lin
We examined the effects of washed platelets (WPLTs) and platelet-rich plasma (PRP) on the proliferation and mineralization of rat dental pulp cells. Rat dental pulp cells were separated, cultured, and identified. Medium containing 1, 10, 100, or 500 mL/L PRP or WPLTs was added to 4th generation cells. The MTS method was used to determine cell proliferatio.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.12
- DOI:
- 10.4238/2015.August.14.12
Isolation and characterization of novel polymorphic microsatellite loci in large yellow croaker (Larimichthys crocea)
Authors: X.Q. Wang, D. Zeng, X. Ma, G. Xiong, Z.Y. Wang and T. Sakamoto
The large yellow croaker (Larimichthys crocea) is one of the largest marine net-cage cultured species in the oceans around China. In the present study, we isolated and characterized 13 polymorphic microsatellite markers from genomic libraries of L. crocea. Loci were screened for 10 wild specimens from 2 sites in southeast of China. All loci were polymorph.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.7
- DOI:
- 10.4238/2015.August.14.7
Efficient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene
Authors: Y.K. Li, C.Z. Fu, Y.R. Zhang and L.S. Zan
In this study, we cloned the coding DNA sequence (CDS) region of Qinchuan cattle LYR motif-containing 1 (LYRM1) and constructed a recombinant adenovirus expression vector to examine the function of LYRM1 on the cellular level. Total RNA was extracted from the adipose tissue of Qinchuan cattle, cDNA was obtained by reverse transcription, and polymerase cha.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.10
- DOI:
- 10.4238/2015.August.14.10
Aquaporin in the proliferation and apoptosis of diabetic myocardial cells
Authors: P.F. Zhao and M.J. Sun
The aim of this study was to explore the effect of aquaporin on the molecular mechanism of human diabetic myocardial cell apoptosis. The methylthiazolyle tetrazolium assay was used to detect the inhibitory effect of different concentrations of aquaporin on cell growth. The rate of aquaporin-induced myocardial cell apoptosis was detected by flow cytometric.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.21.5
- DOI:
- 10.4238/2015.December.21.5
Molecular analysis and conventional cytology: association between HPV and bacterial vaginosis in the cervical abnormalities of a Brazilian population
Authors: A.L. Peres, J.R.S.L. Camarotti, M. Cartaxo, N. Alencar, R.C. Stocco, W. Be�§ak, N.T. Pontes-Filho, R.F.F. Ara�ºjo, J.L. Lima-Filho and D.B.G. Martins2
We investigated the association between bacterial vaginosis (BV) and human papillomavirus (HPV) infection in Papanicolaou smears in a Brazilian population. Cross-sectional analysis was performed on 673 samples collected from women attending public health centers in Olinda (PE, Brazil) by conventionalcytology methodology and molecular analysis, PCR tests (.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.13
- DOI:
- 10.4238/2015.August.14.13
Specialized features of the outer hair cell shapes in the cochlear fovea of bats
Authors: S.Q. Zhang, S.L. Li, H.L. Zhu and L.Y. Yan
In this study, we examined the specialized features of the outer hair cells (OHCs) and the stereocilium bundles of the bat cochlear fovea. Bat cochlea hair cells were observed by scanning and transmission electron microscopy, and the auditory brainstem response thresholds were assessed. The stereocilia bundles of the OHCs were extremely short. The OHC bod.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.16
- DOI:
- 10.4238/2015.August.14.16
Effect of hypothalamic-pituitary-adrenal axis alterations on glucose and lipid metabolism in diabetic rats
Authors: M.W. Si, M.K. Yang and X.D. Fu
This study investigated the relationship between alterations in the hypothalamic-pituitary-adrenal (HPA) axis function and glucose and lipid metabolism in diabetic rats. To accomplish this a diabetes model was established by jointly administering a long-term high-fat diet plus Streptozotocin (STZ; 50 mg/kg ip). The rats were randomly divided into four gro.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.19
- DOI:
- 10.4238/2015.August.14.19
Association between IL-21 polymorphism and systemic lupus erythematosus: a meta-analysis
Authors: J.H. Qi, J. Qi, L.N. Xiang and G. Nie
Several case-control studies have been conducted to investigate the association between Interleukin-21 (IL-21) polymorphisms and systemic lupus erythematosus (SLE) susceptibility, and most of the studies focused on IL-21 rs907715 and rs2221903 polymorphisms. Given the inconsistent results from these studies, the present meta-analysis aimed to obtain a mor.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.14.22
- DOI:
- 10.4238/2015.August.14.22