Human Genetics
Effects of BCL2 transfection on the cell cycle and proliferation of human GES-1 cells
Authors: G.Y. Shi, L.L. Zhao, K. Zhang, H.X. Zhou, A.H. Liu, J. Li, G. Li and L.H. Zhu
We investigated the effects of BCL2 transfection on the cell cycle and proliferation of GES-1 cells. A pcDNA3-BCL2 plasmid was used to transfect GES-1 cell line human gastric epithelial cells. Clones were obtained by G418 screening. BCL2-positive cells were identified by fluorescence immunohistochemistry. The pcDNA3-BCL2 vecto.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.15
- DOI:
- 10.4238/2015.October.5.15
Effects of MK-801 concentration on cell proliferation in rats with focal cerebral ischemia-reperfusion
Authors: Y.K. Gui, L.L. Su, X.L. Niu, P. Zeng, R.R. Fang, H.X. Lv and P. Zhang
We explored the relationship between MK-801 concentration and neural stem cell proliferation in rats with focal cerebral ischemia-reperfusion (FCIR). A total of 60 male Sprague Dawley rats were randomized into control (six rats), sham-operation (six rats), operation (12 rats), and MK-801 groups. The MK-801 group comprised 36 rats that were subjected to di.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.3
- DOI:
- 10.4238/2015.October.21.3
Construction of a hepatocellular carcinoma cell line that stably expresses stathmin with a Ser25 phosphorylation site mutation
Authors: J. Du, Z.H. Tao, J. Li , Y.K. Liu and L. Gan
We constructed hepatocellular carcinoma (HCC) cells that stably express stathmin with a Ser25 phosphorylation site mutation (stathmin S25A). We used the polymerase chain reaction for site-directed mutagenesis, constructed a stathmin S25A plasmid, and verified the results by restriction enzyme cleavage and sequencing technology.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.24
- DOI:
- 10.4238/2015.October.5.24
miR-71b regulation of insulin/IGF-1 signaling during starvation in planarians
Authors: Y.Y. Wu, J.M. Zhao, Q. Liu, Q. Guo, Z. Liu, X.X. Wang, C.Y. Wang, R.Y. Li, Y.Z. Zhang and S.T. Zhang
Planarians, which have a large population of stem cells called neoblasts, are molecularly tractable model systems used in the study of regeneration. However, planarians have strong resistance to hunger and have developed growth arrest strategies. For example, they can change their size and undergo growth regression during star.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.4
- DOI:
- 10.4238/2015.October.5.4
Clinical characteristics of patients with non-small cell lung cancers harboring anaplastic lymphoma kinase rearrangements and primary lung adenocarcinoma harboring epidermal growth factor receptor mutations
Authors: L. Xu, J. Lei, Q.Z. Wang, J. Li and L. Wu
Echinoderm microtubule associated protein like 4-anaplastic lymphoma kinase (EML4-ALK) gene rearrangements and epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer (NSCLC) have been intensively studied. The objective of this study was to determine the clinicopathological characteristics in genotype-specific subsets of patients w.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.18
- DOI:
- 10.4238/2015.October.21.18
LITAF, DUSP1 and HHEX expression in mesenchymal stem cells from patients with psoriasis.
Authors: W.J. Chang, X.P. Niu, R.X. Hou, J.Q. Li, R.F. Liu, Q. Wang1, C.F. Wang,X.H. Li, G.H. Yin and K.M. Zhang
Psoriasis is a common chronic relapsing inflammatory skin disease, in which mesenchymal stem cells (MSCs) have been hypothesized to play an important role in abnormal localized inflammation and vascular proliferation observed in skin lesions. Previous studies have revealed abnormal gene expression patterns, DNA methylation sta.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.31
- DOI:
- 10.4238/2015.December.1.31
Circulating B7-H4 in serum predicts prognosis in patients with hepatocellular carcinoma
Authors: S.A. Zhang, Z.X. Wu, X. Zhang, Z.Y. Zeng and D.L. Li
B7-H4 is member of the B7 family that negatively regulates the immune response, which are important for fine-tuning of the tumor microenvironment. Dysregulation of B7-H4 expression has been associated with tumor progression. However, expression level of B7-H4 in hepatocellular carcinoma (HCC) tissues is still a controversial topic. In addition, whether se.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.25
- DOI:
- 10.4238/2015.October.21.25
Regulation of the expression of zinc finger protein genes by microRNAs enriched within acute lymphoblastic leukemia-derived microvesicles
Authors: L. Lu, X.M. Chen, H.M. Tao, W. Xiong, S.H. Jie and H.Y. Li
Microvesicles (MVs) are submicrometric membrane fragments that can “engulf” cytoplasmic contents such as microRNAs (miRNAs) from their cellular origin. The study of miRNAs carried within MVs might provide insights into the roles that miRNAs play in the underlying pathophysiologic processes of acute lymphoblastic le.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.2
- DOI:
- 10.4238/2015.October.5.2
Inhibition of gap junctions relieves the hepatotoxicity of TNF-�±
Authors: Z.Y. Chen, R. Wang, F. Huang, D.D. Yuan and S.R. Li
The aim of this study was to observe the influence of gap junction (GJ) functional changes on the hepatotoxicity of TNF-α. Three different methods were employed to study functional effects of the GJ inhibition: 1) pretreatment with a GJ inhibitor; 2) inoculation of cells at high and low densities; and 3) inhibition of th.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.3
- DOI:
- 10.4238/2015.October.5.3
Influence of various quantitative trait loci (QTL) mapping methods on the mapping accuracy under varying heritability levels
Authors: C.F. Su, Z.B. Liu and Y.G. Li
The study of quantitative trait effects is of great significance for molecular marker-assisted breeding. The accuracy of quantitative trait loci (QTL) mapping is the key factor affecting marker-assisted breeding, and is extremely significant. The effect of different heritability rates (10, 30, 50, 70, and 90%) on the accuracy of QTL mapping of five recomb.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.21
- DOI:
- 10.4238/2015.October.21.21