Human Genetics
Effect of continuous infusion of midazolam on immune function in pediatric patients after surgery
Authors: H.B. Lu, Y.P. Jia, Z.H. Liang, R. Zhou and J.Q. Zheng
The current study was performed to investigate the effects of midazolam on immune function in pediatric patients after surgery and possible mechanism involved. Patients who needed sedation for more than 2 consecutive days after undergoing surgery in the Pediatric Surgery Department of our hospital were enrolled for the study. Fifty-six patients (5-14 year.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.21.7
- DOI:
- 10.4238/2015.August.21.7
In vitro expansion and differentiation of rat pancreatic duct-derived stem cells into insulin secreting cells using a dynamic three-dimensional cell culture system
Authors: X.C. Chen, H. Liu, H. Li, Y. Cheng, L. Yang and Y.F. Liu
In this study, a dynamic three-dimensional cell culture technology was used to expand and differentiate rat pancreatic duct-derived stem cells (PDSCs) into islet-like cell clusters that can secrete insulin. PDSCs were isolated from rat pancreatic tissues by in situ collagenase digestion and density gradient centrifugation. Usi.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028808
- DOI:
- 10.4238/gmr.15028808
Protein-protein interaction between ezrin and p65 in human breast cancer cells
Authors: R. Tang, F.X. Li, W.F. Shao, Q.S. Wen, X.R. Yu and J.B. Xiong
Our study aimed to investigate the co-localization and protein-protein interactions between ezrin and p65 in human breast cancer cells. Liquid chromatography-mass spectrometry (LCMS) was used to uncover novel protein interactions with ezrin in MDA-MB-231 cells. Endogenous co-immunoprecipitation was used to validate protein-pro.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028334
- DOI:
- 10.4238/gmr.15028334
Comparative quantitative trait locus mapping of maize flowering-related traits in an F2:3 and recombinant inbred line population
Authors: Y.H. Liu, Q. Yi, X.B. Hou, X.G. Zhang, J.J. Zhang, H.M. Liu,Y.F. Hu and Y.B. Huang
Flowering-related traits in maize are affected by complex factors and are important for the improvement of cropping systems in the maize zone. Quantitative trait loci (QTLs) detected using different materials and methods usually vary. In the present study, 266 maize (Zea mays) F2:3 families and 301 recombinant inbred lines (RI.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028465
- DOI:
- 10.4238/gmr.15028465
Detection of protein and DNA damage induced by elevated carbon dioxide and ozone in Triticum aestivum L. using biomarker and comet assay
Authors: E. Abdelhaliem and A.A. Al-Huqail
This study was designed to compare the genetic effects of elevated carbon dioxide (CO2) and ozone (O3), alone or in combination, under irrigated and non-irrigated conditions on proteins and DNA of wheat (Triticum aestivum L.). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isozymes, random amplified poly.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028736
- DOI:
- 10.4238/gmr.15028736
A clinical study evaluating dendritic and cytokine-induced killer cells combined with concurrent radiochemotherapy for stage IIIB non-small cell lung cancer
Authors: X.P. Zhu, Y.H. Xu, J. Zhou and X.F. Pan
To compare the efficacy of dendritic and cytokine-induced killer cells (DC-CIK) therapy combined with concurrent radiochemotherapy on stage IIIB non-small cell lung cancer. Sixty-three patients with stage IIIB non-small cell lung cancer were randomly divided into the study and control groups. The study group, comprising 30 patients, was treated with DC-CI.. Read More»
- Genet. Mol. Res. 14(3):
Roles of microRNA-221/222 in type 2 diabetic patients with post-menopausal breast cancer
Authors: M.Y. Li, S.R. Pan and A.Y. Qiu
The aim of the research was to examine the expression level of microRNA221/222 (miR-221/222) in the serum of patients with type 2 diabetes mellitus (T2DM) who are also diagnosed with post-menopausal breast cancer. We aimed to evaluate the differences in microRNA expression in patients with T2DM alone, patients with post-menopa.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027259
- DOI:
- 10.4238/gmr.15027259
Expression of toll-like receptors in hepatic cirrhosis and hepatocellular carcinoma
Authors: L. Sun, J.J. Dai1, W.F. Hu and J. Wang
Toll-like receptors (TLRs) can specifically identify pathogen-associated molecular patterns (PAMPs) by recognizing structural patterns in diverse microbial molecules, and can provide an effective defense against multiple microbial infectious. A variety of TLRs can be expressed on the surface of liver parenchymal as well as nonparenchymal cells. Kupffer ce.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027419
- DOI:
- 10.4238/gmr.15027419
Predictive potential role of glutathione S-transferase polymorphisms in the prognosis of breast cancer
Authors: X. Wang and Z.H. Huang
The current study aimed at evaluating the association between GSTM1 null/present, GSTT1 null/present, and GSTP1 IIe105Val polymorphisms and clinical response to chemotherapy and treatment outcome of breast cancers patients. Genotyping of GSTP1 rs1695, GSTT1 deletion, and GSTM1 deletion was performed by Polymerase Chain Reaction Restriction Fragment Length.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.28.7
- DOI:
- 10.4238/2015.August.28.7
Inhibitory effect of microRNA-27b on interleukin 17 (IL-17)-induced monocyte chemoattractant protein-1 (MCP1) expression
Authors: K.D. Huang, Y. Shen, X. Wei, F.Q. Zhang, Y.Y. Liu and L. Ma
We investigated the effect of microRNA-27b (miR-27b), a gene expression regulatory factor, on the expression of monocyte chemoattractant protein-1 (MCP1) stimulated by interleukin 17 (IL-17). After IL-17 had been added to H9C2 cardiomyocytes, an miR-27b mimic was transfected into the H9C2 cells. The mRNA expression levels of m.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027784
- DOI:
- 10.4238/gmr.15027784