Human Genetics
Rapid method for DNA extraction from the honey bee Apis mellifera and the parasitic bee mite Varroa destructor using lysis buffer and proteinase K
Authors: M.R.C. Issa1, V.L.C. Figueiredo2, D. De Jong1, C.H. Sakamoto1 and Z.L.P. Sim�µes2
We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, tho.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.4
- DOI:
- 10.4238/2013.October.22.4
Clinical features of diabetes mellitus cases complicated by Burkholderia pseudomallei septicemia
Authors: H.B. Quan, T.Y. Li, Y.Y. Gao and D.X. Chen
The aim of this study was to analyze the clinical characteristics of diabetes mellitus patients with Burkholderia pseudomallei septicemia and evaluate strategies of diagnosis and treatment. The clinical characteristics, diagnosis, treatment, and prognosis of 39 diabetes mellitus patients with B. pseudomallei septicemia were re.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.17.7
- DOI:
- 10.4238/2014.April.17.7
Association analysis of colorectal cancer susceptibility variants with gastric cancer in a Chinese Han population
Authors: C.-P. Zhou, H.-Z. Pan, F.-X Li, N.-Y. Hu, M. Li and X.-X. Yang
Evidence suggests that some genetic variants are risk factors for both colorectal cancer (CRC) and gastric cancer (GC). Thus, we selected 12 reported single nucleotide polymorphisms (SNPs) from genome-wide association studies of CRC and conducted this case-control study to assess the associations between these SNPs and the ris.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.10
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.10
Association of GSTTI and GSTM1 variants with acute myeloid leukemia risk
Authors: Y.M. Zi, S. Wu, D. Ma, C. Yang, M. Yang, Y. Huang and S.J. Yang
We aimed to investigate the relationships between polymorphisms of the glutathione S-transferases (GSTs) GSTM1, GSTTI, and GSTP1 and the risk of developing acute myeloid leukemia (AML). A total of 206 AML cases and 231 controls were collected for our study. The genotyping of GSTs (GSTM1, GSTTI, and GSTP1) was based upon the du.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.11
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.11
Dynamic changes in TAP1 expression levels in newborn to weaning piglets, and its association with Escherichia coli F18 resistance
Authors: J. Wang, Y. Liu, W.H. Dong, Y.J. Huo, X.G. Huang, S.L. Wu and W.B. Bao
The transporter associated with antigen processing (TAP) transports peptides from the cytosol into the endoplasmic reticulum for subsequent loading onto the major histocompatibility complex (MHC) class I molecules. This study showed the dynamic changes in the TAP1 expression level in newborn to weaning piglets. Tissue expressi.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.12
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.12
Association of genetic polymorphisms of SAA1 (rs12218) with myocardial infarction in a Chinese population
Authors: J.W. Wang, H.Q. Wang, W.W. Xiang and T.Y. Chai
We recently cloned MtVP1, a type I vacuolar-type H+-translocating inorganic pyrophosphatase from Medicago truncatula. In the present study, we investigated the cellular location and the function of this H+-PPase in Arabidopsis and potato (Solanum tuberosum L.). An MtVP1::enhanced green fluorescent protein fusion was constructe.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.4
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.4
Association of beta 2 adrenoceptor gene polymorphisms in Malaysian hypertensive subjects
Authors: M. Komara, R. Vasudevan, P. Ismail, S.A. Bakar, S.R. Pishva and F. Heidari
The sympathetic nervous system plays a major role in blood pressure regulation. Beta 2 (β2) adrenoceptor gene polymorphisms have been associated with hypertension in different populations with conflicting results. We examined the association of three common polymorphisms, Arg16Gly, Gln27Glu, and Thr164Ile, of the β2 .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.2
- DOI:
- 10.4238/2014.April.16.2
Expression profiling analysis of hypoxic pulmonary disease
Authors: L. Zhou, L.M. Wang, H.M. Song, Y.Q. Shen, W.J. Xu, J.H. Xu, Y. Liu, W.W. Yan and J.F. Jiang
Exposure of humans to low levels of environmental oxygen results in alveolar hypoxia and normally causes chronic pulmonary hypertension and morphological alterations of precapillary pulmonary vessels. In this study, the microarray dataset GSE11341 was used to identify potential differentially expressed genes related with human lung microvascular endotheli.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.7.2
- DOI:
- 10.4238/2013.October.7.2
Association study of polymorphisms between the Radixin gene and rheumatoid arthritis in a Korean population
Authors: H.-K. Kim, J.-S. Joo, H.-Y Lee, J.-T. Kwon, D.-R. Sohn, S.-J. Hong and H.-J. Kim
Radixin (RDX) is part of the ezrin-radixin-moesin (ERM) protein family. It functions as a membrane-cytoskeletal linker in actin-rich cell surface structures and is thought to be essential for cortical cytoskeleton organization, cell motility, adhesion, and proliferation. An increase in phosphorylated ERM in fibroblast-like syn.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.14
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.14
Expression of ERCC1 and BRCA1 predict the clinical outcome of non-small cell lung cancer in patients receiving platinum-based chemotherapy
Authors: T.B. Wang, N.L. Zhang, S.H. Wang, H.Y. Li, S.W. Chen and Y.G. Zheng
We examined mRNA expression levels of ERCC1, BRCA1, RRM1, and human β-tubulin-III (TUBB3) in non-small-cell lung carcinoma (NSCLC) patients and investigated the association between expression of these genes and the clinical outcome of NSCLC treatment. A total of 366 patients who underwent surgery for NSCLC were included i.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.9.15
- DOI:
- http://dx.doi.org/10.4238/2014.May.9.15