Human Genetics
A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae
Authors: W. Huanca-Mamani, D. Rivera-Cabello and J. Maita-Maita
In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of starting tissue. Maceration with liquid nitrogen, phenol.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.8
- DOI:
- 10.4238/2015.July.17.8
Missense mutation of the EDA gene in a Jordanian family with X-linked hypohidrotic ectodermal dysplasia: phenotypic appearance and speech problems
Authors: O.F. Khabour, F.S. Mesmar, F. Al-Tamimi, O.B. Al-Batayneh and A.I. Owais
Mutations in the EDA gene are responsible for X-linked hypohidrotic ectodermal dysplasia, the most common form of ectodermal dysplasia. Males show a severe form of this disease, while females often manifest mild to moderate symptoms. We identified a missense mutation (c.463C>T) in the EDA gene in a Jordanian family, using d.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr810
- DOI:
- 10.4238/vol9-2gmr810
Genetic structure and genetic diversity of single-variety Lonicera macranthoides populations in China, as indicated by SCoT markers
Authors: D.X. Chen, L.Y. Li, X. Zhang and Y. Wang
Lonicera macranthoides is an important traditional Chinese herb. The lack of information regarding the genetic structure and genetic relationships among its cultivars has hindered the conservation and utilization of this resource. This study used start codon targeted markers to assess the genetic diversity and other genetic characteristics of five single-.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.14
- DOI:
- 10.4238/2015.July.17.14
Correlation of spicule sign on computed tomography scans with peripheral lung cancers associated with interstitial lung disease and chronic obstructive pulmonary disease
Authors: L. Chen, L. Gao and W.L. Wu
The objective of this study was to investigate the correlation between spicular signs on computed tomography (CT) scans and peripheral lung cancer (PLC) that is associated with interstitial lung disease (ILD) and chronic obstructive pulmonary disease (COPD). We analyzed clinical data from 96 PLC cases and grouped patients based on whether they had interst.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.9
- DOI:
- 10.4238/2015.March.27.9
Preliminary in vitro analysis of mechanism of cardiac microvascular endothelial barrier function
Authors: W. Dong, G.N. Zhang and S.H. Gao
To preliminarily clarify the mechanism of cardiac microvascular endothelial barrier function leading to heart failure, primary HMVEC-D cells were selected and cultured for amplification. The cells were infected with adenovirus vector containing the ADP-ribosylation factor 6 (Arf6) Q67L gene. Full-length and functional fragment.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048864
- DOI:
- 10.4238/gmr15048864
A C-type lectin fold gene from Japanese scallop Mizuhopecten yessoensis, involved with immunity and metamorphosis
Authors: X.B. Bao, C.B. He, C.D. Fu, B. Wang, X.M. Zhao, X.G. Gao andW.D. Liu
C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 16.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.11
- DOI:
- 10.4238/2015.March.27.11
IGFBP-3 A-202C and C2133G polymorphisms and colorectal cancer risk: a meta-analysis of case-control studies
Authors: J. Xu, L. Xu, L.T. Li, Q. You and L.S. Cha
Recent evidence suggests that genetic variations in the IGFBP-3 gene may impact susceptibility to colorectal cancer, but individually published results are inconclusive. Our meta-analysis was aimed at providing a more precise estimation of these associations. An extensive literature search was conducted for appropriate articles published before May 1, 201.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.1
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.1
Influence of chitosan nanoparticle-mediated C-erbB-2 gene silencing on invasion and apoptosis of Hep-2 cells
Authors: W.R. Liu, L.R. Cao and G.J. Zuo
We aimed to measure the invasion ability of Hep-2 laryngeal cancer cells after treatment with C-erbB-2-small interfering RNA (siRNA)-chitosan nanoparticles, and assess the applied value of chitosan nanoparticle-mediated C-erbB-2 interference in inhibiting laryngeal cancer invasion and metastasis. Nanoparticles of approximately.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048860
- DOI:
- 10.4238/gmr15048860
Upregulation of salivary �±2 macroglobulin in patients with type 2 diabetes mellitus
Authors: J.K. Feng, Y.F. Lu, J. Li, Y.H. Qi, M.L. Yi and D.Y. Ma
We investigated the expression of salivary α2-macroglobulin (α2-MG) in patients with type 2 diabetes mellitus (T2DM) to investigate its value for predicting damage to the salivary glands. A total of 116 patients with T2DM and 60 patients with impaired fasting glucose (IFG) were included in this study. Sixty health volunteers were enrolled as a.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.12
- DOI:
- 10.4238/2015.March.27.12
Effects of glial cell line-derived neurotrophic factor and leukemia-inhibitory factor on the behavior of two calf testis germline stem cell colony types
Authors: P. Zheng, H. Huang, Y.G. Tian, Y. Zeng, E.O. Adegoke, X. Wang and G.X. Zhang
Germline stem cells are the only such capable of transmitting genetic information in vivo. The isolation and culture of these cells in vitro provide a unique model to understand sperm differentiation and hence, spermatogenesis and male fertility. In this study, we isolated, purified, and cultured germline stem cells from the t.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048856
- DOI:
- 10.4238/gmr15048856