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Cytogenomics

Cytogenomics   Research Article

Characterization and expression of the calpastatin gene in Cyprinus carpio

Authors: W.X. Chen and Y. Ma

Calpastatin, an important protein used to regulate meat quality traits in animals, is encoded by the CAST gene. The aim of the present study was to clone the cDNA sequence of the CAST gene and detect the expression of CAST in the tissues of Cyprinus carpio. The cDNA of the C. carpio CAST gene, amplified using rapid amplification of cDNA ends PCR, is 2834 .. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.16
DOI:
10.4238/2015.July.3.16
Cytogenomics   Research Article

Meiotic behavior and chromosome number of Urochloa adspersa (Trin.) R. D. Webster from the Brazilian Chaco

Authors: M.F. Felismino, R.L.S. Maior, G.A. Damasceno Jr., A. Pott and M.S. Pagliarini

This is the first report of meiotic division in Urochloa adspersa (Trin.) collected from the Brazilian Chaco. Meiotic analyses were performed on three specimens of U. adspersa named G10, G15, and G16. Inflorescences were collected and fixed in a mixture of ethanol and acetic acid (3:1, v/v) for 24 h and then stored in 70% alcohol. Diakinesis revealed diff.. Read More»

Genet. Mol. Res. 14(3):
2015.July.3.21
DOI:
10.4238/2015.July.3.21
Cytogenomics   Review Article

From geochemistry and biochemistry to prebiotic evolution � we necessarily enter into Ganti�s fluid automata

Authors: Hoenigsberg HF

The present study is just an overview of the opening of the geochemical stage for the appearance of life. But that opening would not have been sufficient for the intellectual discovery of the origin of life! The excellent works and many commendable efforts that advance this explanation have not shown the fundamental elements t.. Read More»

Genet. Mol. Res. 6(2):
Cytogenomics   Research Article

Association between polymorphisms in insulin-like growth factor-1 and risk of osteoporosis

Authors: W. Zhang, L.C. Zhang, H. Chen, P.F. Tang and L.H. Zhang

We conducted a case-control study to assess the relationship between rs35767, rs2288377, and rs5742612 insulin-like growth factor-1 (IGF-1) and osteoporosis risk in a Chinese female population. The genotypes of rs35767, rs2288377, and rs5742612 of IGF-1 were determined by polymerase chain reaction-restriction fragment length polymorphism. Patients with os.. Read More»

Genet. Mol. Res. 14(3):
2015.July.13.10
DOI:
10.4238/2015.July.13.10
Cytogenomics   Research Article

Evaluation of hot saline solution and restriction endonuclease techniques in cytogenetic studies of Cycloneda sanguinea L. (Coccinellidae)

Authors: Maffei EMD and Pompolo SG

The goal of the present study was to determine if simple methods, especially hot saline solution (HSS) and MspI and HaeIII restriction endonucleases, which do not require special equipments, may be helpful in studies of genetic variability in the lady beetle, Cycloneda sanguinea. The HSS method extracted the heterochromatin re.. Read More»

Genet. Mol. Res. 6(1):
Cytogenomics   Research Article

An exploratory survey of professionals on the use of stored tissue samples from minors for genetic research

Authors: K. Hens, J. Snoeck, H. Nys, J.-J. Cassiman and K. Dierickx

The ethical aspects of the use of stored tissue samples collected from minors are of topical interest. However, the views of professionals working in the field of genetics have not been investigated in depth anywhere. We conducted a survey among 194 such professionals in Belgium. This list was composed of the members of the Hi.. Read More»

Genet. Mol. Res. 9(2):
vol9-2gmr781
DOI:
10.4238/vol9-2gmr781
Cytogenomics   Research Article

DNA polymorphisms of the Hu sheep melanocortin-4 receptor (MC4R) gene associated with birth weight and 45d-weaning weight

Authors: X.M. Song J.F. Jiang G.Z. Zhang F.X. Shi Y.Q. Jiang

The melanocortin-4 receptor (MC4R) has important roles in regulating food intake, energy balance, and body weight in mammals. In pigs and cattle, MC4R mutations have been identified as genetic markers for growth and traits. Compared with abundant research conducted on other livestock species, little is known about mutations of the ovine MC4R gene. We inve.. Read More»

Genet. Mol. Res. 11(4):
2012.September.27.3
DOI:
10.4238/2012.September.27.3
Cytogenomics   Research Article

Benchmark comparison of ab initio microRNA identification methods and software

Authors: L.L. Hu Y. Huang Q.C. Wang Q. Zou Y. Jiang

MicroRNAs (miRNAs) are short, non-coding RNA molecules that play an important role in the world of genes, especially in regulating the gene expression of target messenger RNAs through cleavage or translational repression of messenger RNA. Ab initio methods have become popular in computational miRNA detection. Most software tools are designed to distinguish m.. Read More»

Genet. Mol. Res. 11(4):
2012.October.17.4
DOI:
10.4238/2012.October.17.4
Cytogenomics   Research Article

Sexual dimorphism of STGC3 tumor suppressor function in nasopharyngeal carcinoma CNE2 cells

Authors: Q. Qiu B. Hu Z.C. Chen X.S. He

STGC3 is a potential tumor suppressor in nasopharyngeal carcinoma. We previously found that CNE2 cells that re-expressed STGC3 formed smaller tumors in female mice than in male mice. Here, we investigated the sexual dimorphism of STGC3 as a tumor-suppressor in female and male nude mice injected subcutaneously with pcDNA3.1(+)-.. Read More»

Genet. Mol. Res. 11(4):
2012.October.9.9
DOI:
10.4238/2012.October.9.9
Cytogenomics   Research Article

Analysis of genetic diversity among wild bermudagrass germplasm from southwest China using SSR markers

Authors: Y. Ling X.-Q. Zhang X. Ma S.-Y. Chen T.-T. Chen W. Liu

Fifty-five wild accessions of bermudagrass (Cynodon dactylon) were collected from southwest China (Sichuan, Chongqing, Yunnan, Guizhou, and Tibet), and their genetic diversity was analyzed using simple sequence repeat markers. A total of 267 polymorphic bands were detected with 18 primer combinations. The genetic similarity among the accessions ranged from 0.. Read More»

Genet. Mol. Res. 11(4):
2012.October.17.5
DOI:
10.4238/2012.October.17.5