Cytogenomics
Conjugated linoleic acid-induced milk fat reduction associated with depressed expression of lipogenic genes in lactating Holstein mammary glands
Authors: L.Q. Han K. Pang H.J. Li S.B. Zhu L.F. Wang Y.B. Wang G.Q. Yang G.Y. Yang
The efficacy of conjugated linoleic acid (CLA) in diet supplements for milk fat reduction is well documented in several species. However, the mechanisms by which fatty acids regulate mammary lipogenesis remain largely unknown, especially with regard to gene expression of enzyme and regulators. In this study, 8 Holstein dairy cows in their mid-lactation pe.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.17.2
- DOI:
- 10.4238/2012.September.17.2
CADM1 mRNA expression and clinicopathological significance in esophageal squamous cell carcinoma tissue
Authors: J.B. Qian, H.B. Liu, Y. Zhu, F. Lu, Q.C. Yang and Y. Shen
The mRNA expression of cell adhesion molecule 1 (CADM1) and its clinicopathological significance in esophageal squamous cell carcinoma (ESCC) tissues were investigated. CADM1 mRNA and protein expression were detected in tissue samples from 50 patients with ESCC by reverse transcription-polymerase chain reaction (RT-PCR) and st.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029178
- DOI:
- 10.4238/gmr16029178
Association of p53 Arg72Pro and MDM2 SNP309 polymorphisms with glioma
Authors: J.N. Zhang S.H. Yi X.H. Zhang X.Y. Liu Q. Mao S.Q. Li W.H. Xiong Y.M. Qiu T. Chen J.W. Ge
Epidemiological studies of the association of variants p53 Arg72Pro and MDM2 single-nucleotide polymorphism 309 (SNP309) with glioma risk have produced inconsistent results. The aim of the current study was to evaluate the association of these 2 variants with glioma susceptibility using a meta-analysis approach. For p53 Arg72Pro, 10 case-control studies i.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.4.9
- DOI:
- 10.4238/2012.October.4.9
An improved protocol and a new grinding device for extraction of genomic DNA from microorganisms by a two-step extraction procedure
Authors: S.S. Zhang, D. Chen and Q. Lu
Current protocols to extract genomic DNA from microorganisms are still laborious, tedious and costly, especially for the species with thick cell walls. In order to improve the effectiveness of extracting DNA from microbial samples, a novel protocol, defined as two-step extraction method, along with an improved tissue-grinding .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.10
- DOI:
- 10.4238/2012.May.21.10
Isolation and identification of the immune-relevant ribosomal protein L10 (RPL10/QM-like gene) from the large yellow croaker Pseudosciaena crocea (Pisces: Sciaenidae)
Authors: X. Chen Y.Q. Su J. Wang M. Liu S.F. Niu S.P. Zhong F. Qiu
In order to investigate the immune role of ribosomal protein L10 (RPL10/QM-like gene) in marine fish, we challenged the large yellow croaker Pseudosciaena (= Larimichthys) crocea, the most important marine fish culture species in China, by injection with a mixture of the bacteria Vibrio harveyi and V. parahaemolyticus (3:1 in volume). Microarray analysis .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.15.7
- DOI:
- 10.4238/2012.October.15.7
Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle
Authors: L.H. Wang W. Zhang Q.X. Gao F. Wang
Luteinizing hormone (LH) is an important glycoprotein hormone that regulates gonadal function in mammals and in turn regulates physiological status changes during the estrous cycle. The function of LH is mediated by luteinizing hormone receptor (LHR). In order to examine the expression patterns of the LHR gene in non-gonadal tissues during the 4 phases of.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.15.8
- DOI:
- 10.4238/2012.October.15.8
Mapping of AFLP loci linked to tolerance to cowpea golden mosaic virus
Authors: M.A. Rodrigues C.A.F. Santos J.R.F. Santana
AFLP markers combined with the bulk segregant analysis methodology was used for the identification of molecular markers associated with the cowpea golden mosaic virus (CGMV) resistance gene in 286 F2 cowpea plants derived from the cross IT97K-499-35 x Canapu T16. Segregation data in the F2 population demonstrated that tolerance to CGMV is controlled by a sin.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.August.17.12
- DOI:
- 10.4238/2012.August.17.12
Does copper stress lead to spindle misposition-dependent cell cycle arrest?
Authors: C. Tarhan A.T. Sarikaya
Because of its specific electrochemical properties, copper is an essential heavy metal for living organisms. As with other heavy metals, high levels can provoke damage. We examined gene expression under copper stress in wild-type fission yeast (Schizosaccharomyces pombe) through differential display. After the EC50 concentration of CuSO4 was determined as 50.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.25.1
- DOI:
- 10.4238/2012.October.25.1
Population genetics of the Pacific abalone (Haliotis discus hannai) in Korea inferred from microsatellite marker analysis
Authors: H.S. An J.W. Lee J.Y. Park
Populations of the Pacific abalone, Haliotis discus hannai, have been severely overexploited over the past few decades in Korea. Information regarding the levels of genetic variability and structure within populations is insufficient for the development of effective strategies for conservation of genetic diversity of this species. To assess the genetic statu.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.12.8
- DOI:
- 10.4238/2012.November.12.8
Analysis of the role of hMLH1 hypermethylation and microsatellite instability in meningioma progression
Authors: M.N. Chen P. Wang J. Zhang B.Y. Zhou Q. Mao Y.H. Liu
We investigated a possible role of hMLH1 hypermethylation and microsatellite instability in meningioma progression. Fifty meningomas were examined for methylation of hMLH1 using a methylation-specific PCR; 43 of them were analyzed for microsatellite instability using nine microsatellite markers. Loss of heterozygosity on chrom.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.August.17.7
- DOI:
- 10.4238/2012.August.17.7