Cytogenomics
Evolutionary analysis of the short-type peptidoglycan-recognition protein gene (PGLYRP1) in primates
Authors: W. Liu Y.F. Yao L. Zhou Q.Y. Ni H.L. Xu
Short-type peptidoglycan (PGN)-recognition protein 1 (PGLYRP1), an innate immunity protein that directly breaks down the structure of microbial cell wall PGNs, plays an important role both in antibacterial defenses and several inflammatory diseases. To explore the adaptive evolution of the PGLYRP1 gene in primates and provide insight into the function of thi.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.8.10
- DOI:
- 10.4238/2013.February.8.10
Adenovirus-mediated interference of FABP4 regulates mRNA expression of ADIPOQ, LEP and LEPR in bovine adipocytes
Authors: S. Wei L.S. Zan H.B. Wang G. Cheng M. Du Z. Jiang G.J. Hausman D.C. McFarland M.V. Dodson
Fatty acid binding protein 4 (FABP4) is an important adipocyte gene, with roles in fatty acid transport and fat deposition in animals as well as human metabolic syndrome. However, little is known about the functional regulation of FABP4 at the cellular level in bovine. We designed and selected an effective shRNA (small hairpin RNA) against bovine FABP4, c.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.4.21
- DOI:
- 10.4238/2013.January.4.21
More severe clinical course of cardiovascular dysfunction in intensive care unit patients with the 894TT eNOS genotype
Authors: L.R. Fraga F.J.O. Paludo A.J.G. B�³s J.L.S. Ferraro F.S. Dias C.S. Alho
The endothelial nitric oxide synthase (eNOS) plays an important homeostatic role in the cardiovascular system (CVS) by maintaining appropriate blood pressure through production of nitric oxide. The 894TT genotype of 894G>T (Glu298Asp, rs1799983), a polymorphic variant of eNOS, has been associated with several vascular diseases. On the basis of this str.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.27.5
- DOI:
- 10.4238/2013.February.27.5
Intracellular localization and association of MHC class I with porcine invariant chain
Authors: F.Z. Xu S.G. Wu W.Y. Yu
The objective was to investigate the intracellular localization and association of pig major histocompatibility complex (MHC) class I subunits with invariant chain (Ii). Pig MHC class I subunit cDNAs were cloned by RT-PCR and eukaryotic expression plasmids of Ã?± and Ã?²2m were constructed with fusions to red or enhanced green fluorescent protein (pDsRed2-.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.11.17
- DOI:
- 10.4238/2013.March.11.17
Genetic variability of watermelon accessions based on microsatellite markers
Authors: R.N.C. de S. Gama C.A.F. Santos R. de C.S. Dias
We analyzed the genetic variability of 40 watermelon accessions collected from 8 regions of Northeastern Brazil using microsatellite markers, in order to suggest strategies of conservation and utilization of genetic variability in this species. These accessions are not commercial cultivars. They were sampled in areas of traditional farmers that usually keep .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.13.3
- DOI:
- 10.4238/2013.March.13.3
Serum lipid abnormalities are not associated with apoB 3' VNTR polymorphism in nephrotic children
Authors: P. Hu B. Hu Y.H. Qin L. Lu Z.Q. Li L.Q. Tao Q. Pei J. Chen
Apolipoprotein B (apoB) gene 3' variable number of tandem repeat (VNTR) is highly variable, and therefore can be an informative marker for associative analysis of lipid metabolism. This is the first report focusing on a possible association of apoB VNTR polymorphism with nephrotic hyperlipidemia. Genomic DNA was extracted from 500 children with primary nephr.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.13.5
- DOI:
- 10.4238/2013.March.13.5
Isolation and phylogenetic analysis of novel �³-gliadin genes in genus Dasypyrum
Authors: G.R. Li C. Liu E.N. Yang Z.J. Yang
As the most ancient member of the wheat gluten family, the Ã?³-gliadin genes are suitable for phylogenetic analysis among wheat and related species. Species in the grass genus Dasypyrum have been widely used for wheat cross breeding. However, the genomic relationships among Dasypyrum species have been little studied. We isolated 22 novel Ã?³-gliadin gene s.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.13.7
- DOI:
- 10.4238/2013.March.13.7
Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress
Authors: J.K. Gan D.X. Zhang D.L. He X.Q. Zhang Z.Y. Chen Q.B. Luo
DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dr.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.1
- DOI:
- 10.4238/2013.March.15.1
Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment
Authors: J.Y. Park Y.S. Yoon H.S. Park S.U. Kuh
We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphat.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.4
- DOI:
- 10.4238/2013.March.15.4
Study of the cytogenetic effects of occupational exposure to pesticides on sanitation workers in Belo Horizonte, Brazil
Authors: F.S.G. Kehdy, E.M.M. Cerqueira, M.B. Bonjardim, R.M. Camelo, M.C.L. Castro
Sanitation workers handling pesticides in the control of disease vectors constitute an occupationally exposed population to genotoxic substances. The aim of the present study was to investigate the relation between the occupational exposure to various pesticides and the presence of cytogenetic damage. Fifty-nine men were selec.. Read More»
- Genet. Mol. Res. 6(3):