Bioinformatics

Development and characterization of microsatellite markers for the walking goby (Scartelaos viridis; Gobiidae)

Authors: D.Q. Sun, H.Y. Li, T.J. Xu and R.X. Wang

Scartelaos viridis (walking goby) is a small edible fish that inhabits warm inshore environments. To provide molecular information of S. viridis, we developed and characterized microsatellite markers for this species. Using (CA)15-enriched genomic libraries of Scartelaos viridis, 44 positive clones were sequenced; 34 sequences.. Read More»

Genet. Mol. Res. 10(1):

vol10-1gmr1015

DOI:

10.4238/vol10-1gmr1015

Critical genes in head and neck squamous cell carcinoma revealed by bioinformatic analysis of gene expression data

Authors: B. Wang, T. Wang, X.L. Cao and Y. Li

In this study, bioinformatic analysis of gene expression data of head and neck squamous cell carcinoma (HNSCC) was performed to identify critical genes. Gene expression data of HNSCC were downloaded from the Cancer Genome Atlas (TCGA) and differentially expressed genes were determined through significance analysis of microarrays. Protein-protein interacti.. Read More»

Genet. Mol. Res. 14(4):

2015.December.21.10

DOI:

10.4238/2015.December.21.10

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

Authors: M.P.C. Telles, F.P. Peixoto, J.S. Lima, L.V. Resende, R.P. Vianello, M.E.M.T. Walter and R.G. Collevatti

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer desig.. Read More»

Genet. Mol. Res. 10(1):

vol10-1gmr1026

DOI:

10.4238/vol10-1gmr1026

Quantitative assessment of protein function prediction programs

Authors: B.N. Rodrigues, M.B.R. Steffens, R.T. Raittz, I.C.R. Santos-Weiss and J.N. Marchaukoski

Fast prediction of protein function is essential for high-throughput sequencing analysis. Bioinformatic resources provide cheaper and faster techniques for function prediction and have helped to accelerate the process of protein sequence characterization. In this study, we assessed protein function prediction programs that accept amino acid sequences as i.. Read More»

Genet. Mol. Res. 14(4):

2015.December.21.28

DOI:

10.4238/2015.December.21.28

Isolation and characterization of 12 polymorphic microsatellite markers for the frog Pelophylax hubeiensis

Authors: J.-C. Yan, F.-H. Zhu and H.-L. Wu

Twelve polymorphic microsatellite loci were isolated from an (AC)n- and (AG)n-enriched DNA library for the endemic Chinese frog Pelophylax hubeiensis (Ranidae). The number of alleles per locus ranged from two to eight, with a mean of 5.17. The observed and expected heterozygosities ranged from 0.226 to 0.839 and from 0.204 to .. Read More»

Genet. Mol. Res. 10(1):

vol10-1gmr1003

DOI:

10.4238/vol10-1gmr1003

Cobalt chloride induces metaphase when topically applied to larvae and pupae of the stingless bee Melipona scutellaris (Hymenoptera, Apidae, Meliponini)

Authors: C. Ueira-Vieira, R.R. Tavares, S. Morelli, B.B. Pereira, R.P. Silva, A.R. Torres-Mariano, W.E. Kerr and A.M. Bonetti

In order to optimize preparations of bee metaphases, we tested cobalt chloride, which has been used as a metaphase inducer in other organisms, such as hamsters and fish. Four microliters of 65 mM cobalt chloride aqueous solution was topically applied to larval and pupal stages of the stingless bee Melipona scutellaris. The cerebral ganglion was removed af.. Read More»

Genet. Mol. Res. 12(2):

2013.February.6.1

DOI:

10.4238/2013.February.6.1

Mitochondrial plasmid: could this be the next hit for genetic engineering?

Authors: S. Ijaz

Genet. Mol. Res. 10(1):

vol10-1gmr1092

DOI:

10.4238/vol10-1gmr1092

Experimental optimization of the number of blocks by means of algorithms parameterized by confidence interval in popcorn breeding

Authors: T.O.M. Paula, C.D. Marinho, A.T. Amaral J�ºnior, L.A. Peternelli and L.S.A. Gon�§alves

The objective of this study was to determine the optimal number of repetitions to be used in competition trials of popcorn traits related to production and quality, including grain yield and expansion capacity. The experiments were conducted in 3 environments representative of the north and northwest regions of the State of Rio de Janeiro with 10 Brazilia.. Read More»

Genet. Mol. Res. 12(2):

2013.June.27.2

DOI:

10.4238/2013.June.27.2

A simplified universal genomic DNA extraction protocol suitable for PCR

Authors: T.Y. Wang, L. Wang, J.H. Zhang and W.H. Dong

Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relative.. Read More»

Genet. Mol. Res. 10(1):

vol10-1gmr1055

DOI:

10.4238/vol10-1gmr1055

An efficient algorithm for computing fixed length attractors based on bounded model checking in synchronous Boolean networks with biochemical applications

Authors: X.Y. Li,G.W. Yang,D.S. Zheng,W.S. Guo and W.N.N. Hung

Genetic regulatory networks are the key to understanding biochemical systems. One condition of the genetic regulatory network under different living environments can be modeled as a synchronous Boolean network. The attractors of these Boolean networks will help biologists to identify determinant and stable factors. Existing methods identify attractors bas.. Read More»

Genet. Mol. Res. 14(2):

http://dx.doi.org/2015.April.28.5

DOI:

http://dx.doi.org/10.4238/2015.April.28.5