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Carbon source-induced changes in the physiology of the cacao pathogen Moniliophthora perniciosa (Basidiomycetes) affect mycelial morphology and secretion of necrosis-inducing proteins
Authors: F.C. Alvim1, E.M. Mattos1, C.P. Pirovani1, K. Gramacho2, C. Pungartnik1, M. Brendel1, J.C.M. Cascardo1 and M. Vincentz3
Quantitative and qualitative relationships were found between secreted proteins and their activity, and the hyphal morphology of Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao. This fungus was grown on fermentable and non-fermentable carbon sources; significant differences in mycelial morphology we.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr619
- DOI:
- 10.4238/vol8-3gmr619
Alpaca fiber growth is mediated by microRNA let-7b via down-regulation of target gene FGF5
Authors: T. Wang, Y. Zhang, H.D. Wang, Y. Shen, N. Liu, J. Cao, X.J. Yu, C.S. Dong and X.Y. He
MicroRNAs are very small endogenous RNA molecules that play a crucial role in an array of biological processes, including regulation of skin morphogenesis. The microRNA let-7b is thought to modulate animal hair growth, by binding target genes that encode growth factors. Fibroblast growth factor 5 (FGF5) has been previously reported to be involved in the i.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.38
- DOI:
- 10.4238/2015.October.28.38
Cloning, characterization and expression analysis of coagulation factor II gene in grass carp (Ctenopharyngodon idella)
Authors: B.H. Xu, K.J. Chen, Y.B. Yao, Q.L. Liu, T.Y. Xiao, J.M. Su and H.Z. Peng
Here, we characterized the structure and function of the coagulation factor II (FII) gene in grass carp and determined its role in coagulation mechanisms. The FII gene EST was obtained using a constructed splenic transcriptome database; the full-length FII gene sequence was obtained by 3' and 5' RACE. The open reading frame (ORF) of FII was cloned and the.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.39
- DOI:
- 10.4238/2015.October.28.39
Molecular cloning and characterization of the pseudorabies virus US1 gene
Authors: M.L. Li J.H. Chen Z.Y. Zhao K.J. Zhang Z. Li J. Li J.Y. Mai X.M. Zhu M.S. Cai
Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated P.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.22.7
- DOI:
- 10.4238/2013.January.22.7
Regularized quantile regression applied to genome-enabled prediction of quantitative traits
Authors: M. Nascimento, F.F. e Silva, M.D.V. de Resende, C.D. Cruz,A.C.C. Nascimento, J.M.S. Viana, C.F. Azevedo, L.M.A. Barroso
Genomic selection (GS) is a variant of marker-assisted selection, in which genetic markers covering the whole genome predict individual genetic merits for breeding. GS increases the accuracy of breeding values (BV) prediction. Although a variety of statistical models have been proposed to estimate BV in GS, few methodologies h.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019538
- DOI:
- 10.4238/gmr16019538
Genetic diversity and selection of common bean lines based on technological quality and biofortification
Authors: S. de M. Steckling, N.D. Ribeiro, F.D. Arns, H.C. Mezzomo, M.T.D.F. Possobom
The development of common bean cultivars with high technological quality that are biofortified with minerals, is required to meet the demand for food with health benefits. The objectives of this study were to evaluate whether common bean genotypes differ in terms of technological and mineral biofortification traits, to study t.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019527
- DOI:
- 10.4238/gmr16019527
Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells
Authors: H.-Y. Liu M.-M. Yang Y.-H. Cui T. Ma X.-Q. Liu L. Bai W.-P. Xiao H.-B. Zhao J.-Y. Peng X.-B. Hu B.-Y. Cao
Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fl.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.4
- DOI:
- 10.4238/2013.January.24.4
REML/BLUP and sequential path analysis in estimating genotypic values and interrelationships among simple maize grain yield-related traits
Authors: T. Olivoto, M. Nardino, I.R. Carvalho3, D.N. Follmann4, M. Ferrari3, V.J. Szareski5, A.J. de Pelegrin3 and V.Q. de Souza6
Methodologies using restricted maximum likelihood/best linear unbiased prediction (REML/BLUP) in combination with sequential path analysis in maize are still limited in the literature. Therefore, the aims of this study were: i) to use REML/BLUP-based procedures in order to estimate variance components, genetic parameters, and .. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019525
- DOI:
- 10.4238/gmr16019525
Demographic characterization of Brazilian patients enrolled in the Fabry Registry
Authors: A.M. Martins S.O. Kyosen J. Garrote F.M.V. Marques J.G. Guilhem E. Macedo J. Sobral Neto S. Ura
Fabry disease (FD) is an X-linked inborn error of metabolism caused by alpha-galactosidase A deficiency. The Fabry Registry is an ongoing observational database that compiles clinical data on patients with FD. We analyzed the Fabry Registry data of patients enrolled in Brazil to characterize the demographic and baseline clinical characteristics of this pa.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.5
- DOI:
- 10.4238/2013.January.24.5
Lack of association between TYK2 and STAT3 genes and Crohn�s disease in the Malaysian population
Authors: L.H. Lian T.P. Lau V.L. Lee W.S. Lee I. Hilmi K.L. Goh K.H. Chua
This study aimed to investigate the potential association of TYK2 and STAT3 genes with the susceptibility to Crohn’s disease (CD) among Malaysians. DNA samples were obtained from 80 CD patients and 100 healthy controls. Polymerase chain reaction-restriction fragment length polymorphism methods were employed for genotyping, followed by statistical an.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.9
- DOI:
- 10.4238/2013.January.24.9