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Characterization of novel nitrate reductase-deficient mutants for transgenic Dunaliella salina systems
Authors: L.J. Gao, Y.L. Jia, S.K. Li and L.L. Qiu
The aim of the present study was to isolate and characterize novel nitrate reductase (NR)-deficient mutants, which may be useful for the transgenic manipulation of Dunaliella salina. Three NR-deficient mutants of D. salina, J-1, J-2, and J-3, were successfully isolated by screening for chlorate resistance after chemical mutagenesis with ethylnitrosourea. .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.25
- DOI:
- 10.4238/2015.October.26.25
Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library
Authors: N.B. Stafuzza C.A. Abbey C.A. Gill J.E. Womack M.E.J. Amaral
River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC li.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.22.6
- DOI:
- 10.4238/2012.May.22.6
Molecular cloning, sequence characterization of a novel pepper gene NADP-ICDH and its effect on cytoplasmic male sterility
Authors: M.H. Deng J.F. Wen J.L. Huo H.S. Zhu X.Z. Dai Z.Q. Zhang H. Zhou X.X. Zou
NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nuc.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.15
- DOI:
- 10.4238/2012.May.18.15
Association between C807T(C/T) polymorphism of platelet glycoprotein gene and sensitivity to ischemic stroke: a meta-analysis
Authors: C. Luo, L.H. Fan, H. Zhang, J. Zhao, L. Li, L. Zhang, H.X. Zhang and M.M. Ma
Ischemic stroke can lead to loss of neurologic functions. It occurs due to obstruction in blood supply to the brain. It has been proposed that C807T(C/T) polymorphism within the platelet glycoprotein gene may be associated with density and function of glycoprotein Ia/IIa receptors and contributes to the pathogenesis of thrombo.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019416
- DOI:
- 10.4238/gmr16019416
Nramp1 gene expression in different tissues of Meishan piglets from newborn to weaning
Authors: C.H. Dai, J.Y. Wu, C.X. Zhao, L.H. Yu, W.B. Bao and S.L. Wu
Natural resistance-associated macrophage protein gene 1 (Nramp1) plays an important role in the innate immune response of swine, and is believed to influence disease resistance. In this study, a real-time quantitative polymerase chain reaction technique was used to investigate Nramp1 expression in 12 different tissues in newbo.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019288
- DOI:
- 10.4238/gmr16019288
Dynamic relationship between SIPA1 gene and protein expression and the development of gastric cancer
Authors: J.Y. Li, J.B. Wang, C.B. Liu, D.L. Ma, J.H. Ma
Association of signal-induced proliferation-associated 1 (SIPA) gene and protein expression with gastric cancer development was examined. SIPA1 mRNA and protein levels were determined by real-time quantitative polymerase chain reaction and western blot, respectively, in 40 gastric tumor and tumor-adjacent normal tissues. SIPA1.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019271
- DOI:
- 10.4238/gmr16019271
Paracrine effect of bone marrow mesenchymal stem cells on proliferation, apoptosis, and alpha-actin-2 expression in hepatic stellate cells
Authors: H.-J. Cao, M.-D. Wang, S.-G. Li, L. Zhu, J.-H. Zheng
We investigated the paracrine effects of bone marrow mesenchymal stem cells (BMSCs) on the proliferation, apoptosis, and alpha-actin-2 (ACTA2) expression of hepatic stellate cells (HSCs), and explored the possible mechanisms of hepatocyte growth factor (HGF). We established a co-culture system by culturing BMSCs on the upper layer and HSCs on the lower la.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019201
- DOI:
- 10.4238/gmr16019201
Multivariate diallel analysis allows multiple gains in segregating populations for agronomic traits in Jatropha.
Authors: P.E. Teodoro, E.V. Rodrigues, L.A. Peixoto, L.A. Silva, B.G. Laviola, L.L. Bhering
Jatropha is research target worldwide aimed at large-scale oil production for biodiesel and bio-kerosene. Its production potential is among 1200 and 1500 kg/ha of oil after the 4th year. This study aimed to estimate combining ability of Jatropha genotypes by multivariate diallel analysis to select parents and crosses that allo.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019545
- DOI:
- 10.4238/gmr16019545
Co-inheritance of variants/mutations in Malaysian patients with Crohn�s disease
Authors: K.H. Chua C.C. Ng I. Hilmi K.L. Goh
Crohn’s disease is a chronic, relapsing inflammatory bowel disease; it affects the mucosa and deeper layers of the digestive wall. Two Crohn’s disease patients who carried the JW1 variant and two patients who carried the SNP5 variant were investigated for other co-inherited polymorphisms that could influence Crohn’s disease development. .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.31.9
- DOI:
- 10.4238/2012.August.31.9
11�²-hydroxysteroid dehydrogenase type 1 gene expression is increased in ascending aorta tissue of metabolic syndrome patients with coronary artery disease
Authors: F. Atalar B. Vural C. Ciftci A. Demirkan G. Akan B. Susleyici-Duman D. Gunay B. Akpinar E. Sagbas U. Ozbek A.S. Buyukdevrim
11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1) activity and mRNA levels are increased in visceral and subcutaneous adipose tissues of metabolic syndrome subjects. We analyzed 11β-HSD-1 expression in human epicardial adipose (EA) and ascending aorta (AA) tissues of metabolic syndrome patients and examined their contribution to the devel.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.31.10
- DOI:
- 10.4238/2012.August.31.10