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MEF2A gene mutations and susceptibility to coronary artery disease in the Chinese population
Authors: J. Li1, H.-X. Chen2, J.-G. Yang3, W. Li3, R. Du3 and L. Tian3
We investigated mutations and polymorphisms of the coronary artery disease (CAD)-related myocyte enhancer factor 2A (MEF2A) gene in a Chinese population. Polymerase chain reaction-single-strand conformation polymorphism and DNA sequencing were used to detect exon 11 of the MEF2A gene in 210 Hubei patients with CAD and 190 healthy controls... Read More»
- Genet. Mol. Res. 13(4):
- /2014.October.20.15
- DOI:
- /10.4238/2014.October.20.15
Correlation between mandibular gland secretion and cuticular hydrocarbons in them stingless bee Melipona quadrifasciata
Authors: C. Cruz-Landim, M.J. Ferreira-Caliman, L.F. Gracioli-Vitti and R. Zucchi
We investigated whether Melipona quadrifasciata worker mandibular gland secretions contribute directly to their cuticular hydrocarbon profile. The mandibular gland secretion composition and cuticular surface compounds of newly emerged worker bees, nurse bees, and foragers were determined by gas chromatography and mass spectrom.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.2
- DOI:
- 10.4238/2012.April.19.2
Cloning flanking sequence by single-primer PCR in transgenic plants
Authors: J. Ma1, Y.P. Wang1, S. Ren2, Z. Zhang1, S. Lu1 and P.W. Wang1
The insertion position of exogenous genes in plant genomes is usually identified by adapter ligation-mediated polymerase chain reaction (PCR), thermal asymmetric interlaced PCR, and restriction site extension PCR in transgenic plant research. However, these methods have various limitations, such as the complexity of designing primers and time-consuming an.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.October.20.16
- DOI:
- 10.4238/2014.October.20.16
AMP-activated protein kinase regulates autophagic protection against cisplatin-induced tissue injury in the kidney
Authors: L. Wei, W. Chen, Y. Zou, H. Huang, B. Pan, S. Jin, R. Huang, S. Nie and G. Kong
Although the nephrotoxicity of cisplatin has been well documented as a major side effect of chemotherapy, the exact mechanism by which prosurvival and apoptotic pathways interplay to determine renal pathology remains elusive. Recent studies suggested that autophagy might serve as an adaptive mechanism to promote cell survival .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.13
- DOI:
- 10.4238/2015.October.5.13
Multivariate analysis in a genetic divergence study of Psidium guajava
Authors: A.M. Nogueira, M.F.S. Ferreira, J.H.S. Guilhen and A. Ferreira
The family Myrtaceae is widespread in the Atlantic Forest and is well-represented in the Espírito Santo State in Brazil. In the genus Psidium of this family, guava (Psidium guajava L.) is the most economically important species. Guava is widely cultivated in tropical and subtropical countries; however, the widespread cultivation of only a small num.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.8
- DOI:
- 10.4238/2014.December.18.8
Leishmania (Viannia) braziliensis in dogs in Brazil: epidemiology, co-infection, and clinical aspects
Authors: F.S. Carvalho, A.A. Wenceslau, G.R. Albuquerque, A.D. Munhoz, E. Gross, P.L.S. Carneiro, H.C. Oliveira, J.M. Rocha, I.A. Santos and R.P. Rezende
Leishmaniasis is an endemic disease present in 98 countries. In Brazil, the northeast region accounts for approximately half of the cases in humans, and has experienced an increased number of positive cases in dogs. In this study, we investigated the epidemiology of canine leishmaniasis in the city of Ilhéus, Bahia, usi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.19
- DOI:
- 10.4238/2015.October.5.19
Identification and bioinformatic analysis of a putative calcium-dependent protein kinase (CDPK6) from Toxoplasma gondii
Authors: N.Z. Zhang, S.Y. Huang, D.H. Zhou, Y. Xu, J.J. He and X.Q. Zhu
Toxoplasma gondii is recognized as an opportunistic human pathogen with a worldwide distribution. Development of effective vaccines is considered the only ideal way to control T. gondii infection. However, only one live vaccine is commercially available for use in sheep and goats. Therefore, the identification of more effective antigenic proteins is very .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.9
- DOI:
- 10.4238/2014.December.18.9
Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance
Authors: M. Lu*, L. Wang*, J. Zhang, S. Sun, Y. Li, W. Du, J. Wu, J. Zhao, Q. Yang and X. Chen
A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The .. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.10
- DOI:
- 10.4238/2014.December.18.10
Phylogenetic relationship of Turkish Apis mellifera subspecies based on sequencing of mitochondrial cytochrome C oxidase I region
Authors: F. ��zdil and F. �°lhan
Mitochondrial DNA sequence variation can be used to infer honey bee evolutionary relationships. We examined DNA sequence diversity in the cytochrome C oxidase I (COI or Cox1) gene segment of the mitochondrial genome in 112 samples of Apis mellifera from 15 different populations in Turkey. Six novel haplotypes were found for th.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.12
- DOI:
- 10.4238/2012.April.27.12
Characterization and expression analysis of somatic embryogenesis receptor-like kinase genes from Phalaenopsis
Authors: Y.W. Huang, Y.J. Tsai and F.C. Chen
Somatic embryogenesis receptor-like kinase (SERK) genes have been found to be involved in the somatic embryogenesis of several plant species. We identified and characterized 5 PhSERK genes in the Phalaenopsis orchid. The amino acid sequences of PhSERKs and other SERK proteins are highly conserved, with the highest homology observed in the leucine-rich rep.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.18.11
- DOI:
- 10.4238/2014.December.18.11