Se
Development of transcript-associated microsatellite markers in Ancherythoculter nigrocauda and cross-amplification in Culter alburnus
Authors: Y. Sun1,2, Q. Li1, G. Wang1, D. Zhu1, J. Chen1 and P. Li1
Twenty-eight polymorphic microsatellite markers were developed from the transcriptome of Ancherythoculter nigrocauda. These loci were used to characterize the genotypes of 48 individuals. The observed number of alleles per locus ranged from 5 to 11, with an average of 7.7. Expected and observed heterozygosities ranged from 0.437 to 0.978 and from 0.373 to.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.12
- DOI:
- 10.4238/2015.November.13.12
Construction of a cDNA library and preliminary analysis of expressed sequence tags in Piper hainanense
Authors: R. Fan, P. Ling, C.Y. Hao, F.P. Li, L.F. Huang, B.D. Wu and H.S. Wu
Black pepper is a perennial climbing vine. It is widely cultivated because its berries can be utilized not only as a spice in food but also for medicinal use. This study aimed to construct a standardized, high-quality cDNA library to facilitated identification of new Piper hainanense transcripts. For this, 262 unigenes were us.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.17
- DOI:
- 10.4238/2015.October.19.17
Effect of progranulin (PGRN) on the proliferation and senescence of cervical cancer cells
Authors: X.Y. Zhang1, Z.X. Pan1, H. Liu1, J.L. Yu1, G.X. Li2, H.Y. Wang3 and M.M. Liu4
We investigated the effect of progranulin (PGRN) expression on the proliferation and senescence of cervical cancer cells. PGRN small interfering RNA (siRNA) was introduced into the SiHa and HeLa cell lines of human cervical carcinoma using liposome-mediated transfection. The expression levels of PGRN in each cell line after transfection of PGRN siRNA were.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.18
- DOI:
- 10.4238/2015.November.13.18
Identification of differently expressed genes in leukemia using multiple microarray datasets
Authors: Z.Y. Zhang, R.Q. Xu, T.J. Guo, M. Zhang, D.X. Li and X.Y. Lu
The purpose of this study was to identify differentially expressed genes and analyze biological processes related to leukemia. A meta-analysis was performed using the Rank Product package of Gene Expression Omnibus datasets for leukemia. Next, Gene Ontology-enrichment analysis and pathway analysis were performed using the Gene Ontology website and Kyoto E.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.9
- DOI:
- 10.4238/2014.December.12.9
Avian sarcoma and leukosis virus gag gene in the Anser anser domesticus genome
Authors: F. Zhu1, H. Jie3, L. Lian2, L.J. Qu2, Z.C. Hou2, J.X. Zheng2, S.Y. Chen1,N. Yang2 and Y.P. Liu1
Endogenous retroviruses are regarded as ideal genetic markers for evolutionary analyses. Birds were some of the initial vertebrates found to contain endogenous retroviruses. However, few studies have investigated the presence and distribution of endogenous retroviruses in goose. In this study, we detected the avian sarcoma and leukosis virus gag gene in t.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.1
- DOI:
- 10.4238/2015.November.18.1
Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers
Authors: L.G. Zhang, Y. Chang, X.F. Zhang, F.Z. Guan, H.M. Yuan, Y. Yu and L.J. Zhao
Hemp (Cannabis sativa) is an important fiber crop, and native cultivars exist widely throughout China. In the present study, we analyzed the genetic diversity of 27 important Chinese native hemp cultivars, by using inter-simple sequence repeats (ISSR) and chromosome markers. We determined the following chromosome formulas: 2n = 20 = 14m + 6sm; 2n = 20 = 2.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.10
- DOI:
- 10.4238/2014.December.12.10
Presence of Mycobacterium leprae DNA and PGL-1 antigen in household contacts of leprosy patients from a hyperendemic area in Brazil
Authors: J.D. Pinho1, P.M.S. Rivas1, M.B.P. Mendes1, R.E.P. Soares1, G.C. Costa2,F.R.F. Nascimento2, M.F.L. Paiva3, D.M.C. Aquino4, I.A. Figueireido5,A.M. Santos3 and S.R.F. Pereira1
Leprosy is a highly infectious disease endemic to underdeveloped countries. In Maranhão State, Northeastern Brazil, the hyperendemic rate of 56.11 cases/100,000 inhabitants increased the necessity of better understanding the epidemiological profile of this population, particularly regarding efficient methods for evaluating individuals res.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.10
- DOI:
- 10.4238/2015.November.18.10
Genetic parameters for milk production traits and breeding goals for Gir dairy cattle in Brazil
Authors: M.A. Prata, L.E. Faro, H.L. Moreira, R.S. Verneque, A.E. Vercesi Filho, M.G.C.D. Peixoto and V.L. Cardoso
To implement an animal breeding program, it is important to define the production circumstances of the animals of interest to determine which traits of economic interest will be selected for the breeding goal. The present study defined breeding goals and proposed selection indices for milk production and quality traits of Gir .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.2
- DOI:
- 10.4238/2015.October.19.2
Genetic diversity and relationship analysis of Gossypium arboreum accessions
Authors: F. Liu1,2, Z.L. Zhou2, C.Y. Wang2, Y.H Wang2, X.Y. Cai2, X.X. Wang2,Z.S. Zhang1 and K.B. Wang2
Simple sequence repeat techniques were used to identify the genetic diversity of 101 Gossypium arboreum accessions collected from India, Vietnam, and the southwest of China (Guizhou, Guangxi, and Yunnan provinces). Twenty-six pairs of SSR primers produced a total of 103 polymorphic loci with an average of 3.96 polymorphic loci per primer. The average of t.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.15
- DOI:
- 10.4238/2015.November.18.15
Cloning and characterization of the SERK1 gene in triploid Pingyi Tiancha [Malus hupehensis (Pamp.) Rehd. var. pingyiensis Jiang] and a tetraploid hybrid strain
Authors: L.J. Zhang1,3, W.X. Dong1, S.M. Guo1, Y.X. Wang2, A.D. Wang1 and X.J. Lu3
This study aims to explore the roles of somatic embryogenesis receptor-like kinase (SERK) in Malus hupehensis (Pingyi Tiancha). The full-length sequences of SERK1 in triploid Pingyi Tiancha (3n) and a tetraploid hybrid strain 33# (4n) were cloned, sequenced, and designated as MhSERK1 and MhdSERK1, respectively. Multiple alignments of amino acid sequences .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.21
- DOI:
- 10.4238/2015.November.18.21