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Effects of kinase insert domain receptor (KDR) gene silencing on the sensitivity of A549 cells to erlotinib
Authors: W.L. Zhu and Y.H. Liu
We investigated the effects of kinase insert domain receptor (KDR) gene silencing on the proliferation of A549 cells and their sensitivity to erlotinib. A KDR small interfering RNA (siRNA) sequence was designed and synthesized; then, it was transfected into A549 cells using LipofectamineTM 2000. KDR mRNA and protein expression after KDR gene si.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.15
- DOI:
- 10.4238/2015.November.24.15
Genetic structure in fragmented populations of Solanum lycocarpum A. St.-Hil. with distinct anthropogenic histories in a Cerrado region of Brazil
Authors: T.M. Moura, K. Martins, P.S. Sujii, A.M. Sebbenn and L.J. Chaves
Solanum lycocarpum is a woody tree widely distributed in the Cerrado that reaches high population densities in disturbed environments. We examined the genetic diversity and population differentiation of six S. lycocarpum populations with different degrees of human disturbance in order to determine if they are negatively affected by anthropogenic activity... Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.16
- DOI:
- 10.4238/2012.July.10.16
Analysis of an â??off-ladderâ? allele at the Penta D short tandem repeat locus
Authors: Y.L. Yang, J.G. Wang, D.X. Wang, W.Y. Zhang, X.J. Liu, J. Cao and S.L. Yang
Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.18
- DOI:
- 10.4238/2015.November.24.18
Detection of CK19, LUNX, and KS1/4 mRNA expression in the peripheral blood for diagnosis of micrometastases in patients with non-small cell lung cancer and their clinical implications
Authors: L. Yan, Y. Yao, L.H. Wang, M.L. Wang and X.H. Fu
The expression of CK19, LUNX, and KS1/4 mRNA biomarkers was detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients to investigate the feasibility of indicating lung cancer micrometastases. Micrometastases were identified in the peripheral blood of 32 NSCLC patients, 15 benign pulmonar.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.24.17
- DOI:
- 10.4238/2015.November.24.17
Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae
Authors: T. Jin, L. Zeng, Y.-Y. Lin, Y.-Y. Lu and G.-W. Liang
Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-tr.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.12
- DOI:
- 10.4238/2012.July.10.12
Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape
Authors: X.Q. Huang, R.Q. He, X.Y. Liao, B. Zhou, W.S. Peng, J.Z. Lin, D.Y. Tang, Y.H. Zhu, X.Y. Zhao and X.M. Liu
Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed ra.. Read More»
- Genet. Mol. Res. 13(2):
Genetic diversity and population structure of Chinese pony breeds using microsatellite markers
Authors: L.X. Xu, S.L. Yang, R.Y. Lin, H.B. Yang, A.P. Li and Q.S. Wan
China is one of the principal origins of ponies in the world. We made a comprehensive analysis of genetic diversity and population structure of Chinese ponies based on 174 animals of five indigenous Chinese pony breeds from five provinces using 13 microsatellite markers. One hundred and forty-four alleles were detected; the mean number of effective allele.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.25.4
- DOI:
- 10.4238/2012.June.25.4
Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells
Authors: Q.D. Jiang, H.P. Li, F.J. Liu, X.J. Wang, Y.J. Guo, L.F. Wang, W.F. Lu, H.J. Li, X.P. Li and Y.Y. Wang
This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 106 cells per dish). The test was divided into 6 group.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.24.12
- DOI:
- 10.4238/2014.January.24.12
Identification and analysis of a 5-bp indel of a porcine BMP7 gene promoter
Authors: P. Lu, X. Feng, J.S. Zhou, X.B. Liu, Q.X. Chen and M. Li
Bone morphological protein7 (BMP7), a member of the transforming growth factor-β (TGF-β) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ov.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.19
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.19
Identification and characterization of polymorphic microsatellite loci in the red-crowned crane
Authors: L. Zhang, Z.H. Zhang, F.J. Shen, R. Hou, W.P. Zhang, Y.L. Liu, K.Y. Tu and A.L. Yang
We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes... Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.5
- DOI:
- 10.4238/2015.November.25.5