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Microbial Genetics   Research Article

Genetic structure in fragmented populations of Solanum lycocarpum A. St.-Hil. with distinct anthropogenic histories in a Cerrado region of Brazil

Authors: T.M. Moura, K. Martins, P.S. Sujii, A.M. Sebbenn and L.J. Chaves

Solanum lycocarpum is a woody tree widely distributed in the Cerrado that reaches high population densities in disturbed environments. We examined the genetic diversity and population differentiation of six S. lycocarpum populations with different degrees of human disturbance in order to determine if they are negatively affected by anthropogenic activity... Read More»

Genet. Mol. Res. 11(3):
2012.July.10.16
DOI:
10.4238/2012.July.10.16
Medical Genetics   Research Article

Analysis of an â??off-ladderâ? allele at the Penta D short tandem repeat locus

Authors: Y.L. Yang, J.G. Wang, D.X. Wang, W.Y. Zhang, X.J. Liu, J. Cao and S.L. Yang

Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than .. Read More»

Genet. Mol. Res. 14(4):
2015.November.24.18
DOI:
10.4238/2015.November.24.18
Medical Genetics   Research Article

Detection of CK19, LUNX, and KS1/4 mRNA expression in the peripheral blood for diagnosis of micrometastases in patients with non-small cell lung cancer and their clinical implications

Authors: L. Yan, Y. Yao, L.H. Wang, M.L. Wang and X.H. Fu

The expression of CK19, LUNX, and KS1/4 mRNA biomarkers was detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients to investigate the feasibility of indicating lung cancer micrometastases. Micrometastases were identified in the peripheral blood of 32 NSCLC patients, 15 benign pulmonar.. Read More»

Genet. Mol. Res. 14(4):
2015.November.24.17
DOI:
10.4238/2015.November.24.17
Microbial Genetics   Research Article

Characteristics of protein variants in trichlorphon-resistant Bactrocera dorsalis (Diptera; Tephritidae) larvae

Authors: T. Jin, L. Zeng, Y.-Y. Lin, Y.-Y. Lu and G.-W. Liang

Functional proteins in larvae of Bactrocera dorsalis, a major fruit pest, play a central role in their resistance to organophosphorus insecticides. Changes in proteins in B. dorsalis larvae after trichlorphon treatment may have a role in the resistance response to trichlorphon. We analyzed 14 protein spots of crude proteins from B. dorsalis larvae post-tr.. Read More»

Genet. Mol. Res. 11(3):
2012.July.10.12
DOI:
10.4238/2012.July.10.12
Plant Genetics   Research Article

Effect of exogenous gibberellin on reserve accumulation during the seed filling stage of oilseed rape

Authors: X.Q. Huang, R.Q. He, X.Y. Liao, B. Zhou, W.S. Peng, J.Z. Lin, D.Y. Tang, Y.H. Zhu, X.Y. Zhao and X.M. Liu

Exogenous gibberellins (GAs) are widely applied to increase crop yields, with knowledge about the physiological functioning and biochemistry mechanisms of these phytohormones improving; however, information remains limited about the effect of GAs on seed filling. In this study, the siliques (containing the seeds) of oilseed ra.. Read More»

Genet. Mol. Res. 13(2):
Bioinformatics   Research Article

Genetic diversity and population structure of Chinese pony breeds using microsatellite markers

Authors: L.X. Xu, S.L. Yang, R.Y. Lin, H.B. Yang, A.P. Li and Q.S. Wan

China is one of the principal origins of ponies in the world. We made a comprehensive analysis of genetic diversity and population structure of Chinese ponies based on 174 animals of five indigenous Chinese pony breeds from five provinces using 13 microsatellite markers. One hundred and forty-four alleles were detected; the mean number of effective allele.. Read More»

Genet. Mol. Res. 11(3):
2012.June.25.4
DOI:
10.4238/2012.June.25.4
Bioinformatics   Research Article

Effects of lipopolysaccharide on the stearoyl-coenzyme A desaturase mRNA level in bovine primary hepatic cells

Authors: Q.D. Jiang, H.P. Li, F.J. Liu, X.J. Wang, Y.J. Guo, L.F. Wang, W.F. Lu, H.J. Li, X.P. Li and Y.Y. Wang

This study aimed to compare the effects of lipopolysaccharide (LPS) on stearoyl-coenzyme A desaturase (SCD) gene expression in mouse primary hepatic cells. To obtain sufficient total RNA, primary hepatic cells were plated on 6-cm diameter-type collagen 1-coated dishes (1 x 106 cells per dish). The test was divided into 6 group.. Read More»

Genet. Mol. Res. 13(2):
2014.January.24.12
DOI:
10.4238/2014.January.24.12
Animal Genetics   Research Article

Identification and analysis of a 5-bp indel of a porcine BMP7 gene promoter

Authors: P. Lu, X. Feng, J.S. Zhou, X.B. Liu, Q.X. Chen and M. Li

Bone morphological protein7 (BMP7), a member of the transforming growth factor-β (TGF-β) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ov.. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.June.9.19
DOI:
http://dx.doi.org/10.4238/2014.June.9.19
Animal Genetics   Research Article

Identification and characterization of polymorphic microsatellite loci in the red-crowned crane

Authors: L. Zhang, Z.H. Zhang, F.J. Shen, R. Hou, W.P. Zhang, Y.L. Liu, K.Y. Tu and A.L. Yang

We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes... Read More»

Genet. Mol. Res. 14(4):
2015.November.25.5
DOI:
10.4238/2015.November.25.5
Plant Genetics   Research Article

Structural and phylogenetic analysis of Pto-type disease resistance gene candidates in Hevea brasiliensis

Authors: W. Zhai, Y. Zhao, L.X. Zhang and X.J. Li

The tomato Pto gene encodes a serine/threonine kinase (STK) whose molecular characterization has provided valuable insights into the disease resistance mechanism of tomato. Therefore, Pto is considered as a promising candidate for engineering broad-spectrum pathogen resistance in this crop. In this study, a pair of degenerate .. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.June.10.2
DOI:
http://dx.doi.org/10.4238/2014.June.10.2