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Correlation analysis between starch properties and single nucleotide polymorphisms of waxy genes in common rye (Secale cereale L.)
Authors: M. Meng, X. Gao, L.J. Han, X.Y. Li, D. Wu, H.Z. Li and Q.J. Chen
To understand the relationships between single nucleotide polymorphisms (SNPs) in the waxy gene and starch parameters in common rye, we performed sequence characterization, enzyme activity testing, amylopectin/amylose ratio evaluation, starch property testing, and correlation analysis. Specific primers were used to clone waxy .. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.14.7
- DOI:
- 10.4238/2014.January.14.7
Biolistic transformation of wheat using the HMW-GS 1Dx5 gene without selectable markers
Authors: J.B. Qin, Y. Wang and C.Q. Zhu
Genetic manipulation using linear DNA was applied to the common wheat variety Xindong No. 26 via particle bombardment with the aim to improve bread-making quality of flour. Initially, 2 biolistic parameters, helium pressure and target distance, were optimized using plasmid pAHC25. We transformed wheat immature embryo scutella .. Read More»
- Genet. Mol. Res. 13(2):
Nested polymerase chain reaction amplification and sequencing analysis of the light-chain and heavy-chain variable regions in the influenza A H1N1 virus hemagglutinin monoclonal antibody gene
Authors: H.J. Li, C.Y. Guo, J.Y. Sun, L.J. Sun, P.H. Zhao, L. Hu, Y. Li and J. Hu
The nested polymerase chain reaction (PCR) method was used for the amplification of the influenza A H1N1 virus hemagglutinin monoclonal antibody light-chain and heavy-chain genes. Sequence analysis of the obtained genes was then used to identify common cloning methods of the mouse immunoglobulin-kappa (Igκ) light-chain a.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.11.1
- DOI:
- http://dx.doi.org/10.4238/2014.June.11.1
Cryopreservation does not alter karyotype, multipotency, or NANOG/SOX2 gene expression of amniotic fluid mesenchymal stem cells
Authors: P.C. Ã?Â?ngelo, A.C.S. Ferreira, V.D. Fonseca, S.P. Frade, C.S. Ferreira, F.S.V. Malta, A.K. Pereira, H.V. Leite, A.P. Brum, V.C. Pardini, K.B. Gomes and A.C.V. Cabral
Cryopreservation of mesenchymal stem cells from amniotic fluid is of clinical importance, as these cells can be harvested during the prenatal period and stored for use in treatments. We examined the behavior of mesenchymal stem cells from human amniotic fluid in culture that had been subjected to cryopreservation. We assessed .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.19.5
- DOI:
- 10.4238/2012.April.19.5
Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism
Authors: T. Kanchanaketu, N. Sangduen, T. Toojinda and V. Hongtrakul
Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.13.2
- DOI:
- 10.4238/2012.April.13.2
High methylation of the SEPT9 gene in Chinese colorectal cancer patients
Authors: X.L. Su, Y.F. Wang, S.J. Li, F. Zhang and H.W. Cui
Methylation of the septin 9 gene (SEPT9) occurs in higher frequency in colorectal cancer (CRC) compared to control samples, which suggests that SEPT9 methylation is a useful biomarker for screening CRC. However, the methylation status of SEPT9 in Chinese CRC patients is scarcely reported. In the present study, SEPT9 methylatio.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.January.17.5
- DOI:
- 10.4238/2014.January.17.5
Relationship between perioperative cardiovascular risk factors and bone marrow cells from patients undergoing coronary artery bypass grafting surgery
Authors: L. Zhang, R. Wang, C.-S. Xiao, Y. Wu and C.-Q. Gao
Cell therapy through the implantation of autologous bone marrow cells has long been used in clinical trials for the treatment of ischemic heart diseases. However, as the outcomes of cell implantation vary among patients, risk factors that might influence the level and function of bone marrow progenitor cells should be determin.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.11
- DOI:
- 10.4238/2015.November.25.11
Methylation-sensitive amplified polymorphism-based genome-wide analysis of cytosine methylation profiles in Nicotiana tabacum cultivars
Authors: J. Jiao, J. Wu, Z. Lv, C. Sun, L. Gao, X. Yan, L. Cui, Z. Tang, B. Yan and Y. Jia
This study aimed to investigate cytosine methylation profiles in different tobacco (Nicotiana tabacum) cultivars grown in China. Methylation-sensitive amplified polymorphism was used to analyze genome-wide global methylation profiles in four tobacco cultivars (Yunyan 85, NC89, K326, and Yunyan 87). Amplicons with meth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.25.6
- DOI:
- 10.4238/2015.November.25.6
Classification of colon cancer based on the expression of randomly selected genes
Authors: X.H. Tan, R. Cheng, H.P. Hu and Y.P. Bai
In order to ascertain the relationship between gene expression and colon cancer localization, a classification method based on random gene selection and a self-organizing map network is proposed. Different numbers of genes were selected randomly from 54,675 genes of 53 colon cancer patients in stage union for international can.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.6
- DOI:
- 10.4238/2015.October.19.6
Thymidylate synthase enhancer region polymorphism not related to susceptibility to acute lymphoblastic leukemia in the Kashmir population
Authors: F.H. Nazki, A. Masood, M.A. Banday, A. Bhat and B.A. Ganai
Thymidylate synthase (TS) is a crucial enzyme in folate metabolism and plays a vital role in DNA synthesis and repair. The most common polymorphism in TS is a unique double (2R) or triple (3R) 28-bp tandem repeat sequence in the enhancer region of the TS gene (TSER). This genetic variation in TSER has been widely investigated .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.6
- DOI:
- 10.4238/2012.April.10.6