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Isolation and characterization of microsatellite markers for the Korean rockfish, Sebastes schlegeli
Authors: C.-C. Bai, S.-F. Liu, Z.-M. Zhuang, L. Lin, H. Wang and Y.-Q. Chang
The Korean rockfish (Sebastes schlegeli) is an important commercial fish that is widely used in aquaculture. We isolated and characterized 18 polymorphic microsatellite loci from the Korean rockfish using a (GT)13-enriched genomic library. Polymorphism was assessed in 48 individuals from a single population collected from the .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1522
- DOI:
- 10.4238/vol10-3gmr1522
Characterization of the �²-1,3-glucanase gene in peanut (Arachis hypogaea L.) by cloning and genetic transformation
Authors: L.X. Qiao, X. Ding, H.C. Wang, J.M. Sui and J.-S. Wang
Plant b-1,3-glucanases are commonly involved in disease resistance. This report describes the cloning and genetic transformation of a b-1,3-glucanase gene from peanut. The gene was isolated from both the genomic DNA and cDNA of peanut variety Huayu20 by polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), re.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.17
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.17
genetic evaluation of the HSP70 protein in the japanese quail (Coturnix japonica)
Authors: H.C.T. Gaviol, E. Gasparino, A.J. Prioli and M.A.M. Soares
Heat stress is one of the main problems in modern aviculture, since it affects birds especially in the final phase of rearing, causing bird mortality and economic losses to the aviculturist. The quail, as most birds, has difficulties in dissipating heat. However, little is known about the mechanism that controls the responses of the organism to stressor a.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr354
- DOI:
- 10.4238/vol7-1gmr354
Isolation and characterization of polymorphic microsatellite loci of the Chinese muntjac (Muntiacus reevesi)
Authors: H. Wang, X. Luo, W.B. Shi and B.W. Zhang
Eight polymorphic microsatellite markers for Muntiacus reevesi were identified and characterized in this study. The number of alleles per locus ranged from 3 to 10 across 24-48 samples. The loci showed expected and observed heterozygosities of 0.577-0.876 and 0.387-0.933, respectively, with an average polymorphic information c.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.18
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.18
Effect of the beta secretase-1 inhibitor on the amyloid C-terminal fragment of amyloid precursor protein processing in a hyperphosphorylated tau rat model
Authors: H.Y. Chen, L. Wang, J.F. Liu, W.Z. Wang and C.J. Yu
The amyloid C-terminal fragment (βCTF) of the amyloid precursor protein (APP) is the cleaved component of APP by beta secretase-1 (BACE1), which shows similar neurotoxicity as amyloid beta (Aβ) in many ways. Evidence suggested that in addition to Aβ, βCTF might also participate in the pathogenesis of Alzhei.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.15.4
- DOI:
- 10.4238/2014.August.15.4
Detection by denaturing gradient gel electrophoresis of ammonia-oxidizing bacteria in microcosms of crude oil-contaminated mangrove sediments
Authors: A.C.F. dos Santos, E.L.S. Marques, E. Gross, S.S. Souza, J.C.T. Dias, M. Brendel and R.P. Rezende
Currently, the effect of crude oil on ammonia-oxidizing bacterium communities from mangrove sediments is little understood. We studied the diversity of ammonia-oxidizing bacteria in mangrove microcosm experiments using mangrove sediments contaminated with 0.1, 0.5, 1, 2, and 5% crude oil as well as non-contaminated control and.. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.27.6
- DOI:
- http://dx.doi.org/10.4238/2012.January.27.6
A transient assay for recombination demonstrates that Arabidopsis SNM1 and XRCC3 enhance non-homologous recombination
Authors: R.A. Johnson, R.P. Hellens and D.R. Love
Replacement of endogenous genes by homologous recombination is rare in plants; the majority of genetic modifications are the result of transforming DNA molecules undergoing random genomic insertion by way of non-homologous recombination. Factors that affect chromatin remodeling and DNA repair are thought to have the potential .. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1347
- DOI:
- 10.4238/vol10-3gmr1347
Molecular variability in Brycon cf. pesu M�¼ller and Troschel, 1845 (Characiformes: Characidae) from the Araguaia-Tocantins basin
Authors: R.S. Panarari-Antunes, A.J. Prioli, S.M.A.P. Prioli, H.F. J�ºlio Jr. C.S. Agostinho and L.M. Prioli
Brycon pesu is a small-sized fish distributed throughout the Amazon and Orinoco Basins and other coastal basins of northeastern South America. Brycon cf. pesu specimens from the Araguaia-Tocantins Basin are currently separated into two morphotypes, Brycon sp1 and Brycon sp2, owing to different coloration of their anal fin. Brycon sp2 has a reddish margin .. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr365
- DOI:
- 10.4238/vol7-1gmr365
A simple boiling-based DNA extraction for RAPD profiling of landfarm soil to provide representative metagenomic content
Authors: J.H. Amorim, R.O. Vidal, G.V. Lacerda-Junior, J.C.T. Dias, M. Brendel, R.P. Rezende and J.C.M. Cascardo
Landfarm soils are employed in industrial and petrochemical residue bioremediation. This process induces selective pressure directed towards microorganisms capable of degrading toxic compounds. Detailed description of taxa in these environments is difficult due to a lack of knowledge of culture conditions required for unknown .. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.27.5
- DOI:
- http://dx.doi.org/10.4238/2012.January.27.5
Improved method for predicting protein fold patterns with ensemble classifiers
Authors: W. Chen, X. Liu, Y. Huang, Y. Jiang, Q. Zou and C. Lin
Protein folding is recognized as a critical problem in the field of biophysics in the 21st century. Predicting protein-folding patterns is challenging due to the complex structure of proteins. In an attempt to solve this problem, we employed ensemble classifiers to improve prediction accuracy. In our experiments, 188-dimension.. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.27.4
- DOI:
- http://dx.doi.org/10.4238/2012.January.27.4