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Hybridization

Human Genetics   Research Article

Successful preimplantation genetic aneuploidy screening in Turkish patients

Authors: N. Ercelen, E. Turtar, M. Gultomruk, H. Comert, H. Coskun, R. Mercan and A. Nuhoglu

Preimplantation genetic diagnosis is a preventive approach for identifying genetic abnormalities in early stages of reproduction. We used preimplantation genetic aneuploidy screening in 230 cycles of patients with indications of advanced maternal age, recurrent implantation failure, recurrent spontaneous abortions, or severe m.. Read More»

Genet. Mol. Res. 10(4):
2011.November.17.6
DOI:
10.4238/2011.November.17.6
Plant Genetics   Research Article

Molecular cytogenetic characterization of a new leaf rolling triticale

Authors: E.N. Yang, Z.J. Yang, J.F. Zhang, Y.C. Zou and Z.L. Ren

Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed pro.. Read More»

Genet. Mol. Res. 10(4):
2011.November.29.6
DOI:
10.4238/2011.November.29.6
Human Genetics   Research Article

Sperm aneuploidy and implications for genetic counseling in a pedigree of three t(1;3) balanced translocation carriers

Authors: L.-L. Li, Y. Dong, R.-X. Wang, N. An, X. Yun and R.-Z. Liu

A reciprocal translocation between the short arm of chromosome 1 and the long arm of chromosome 3 was observed in a pedigree of three carriers (proband, and his brother and mother). In this study, the three carriers had different clinical manifestations: the proband with infertility, his brother with spousal miscarriages, and his mother with no adverse re.. Read More»

Genet. Mol. Res. 14(2):
2015.May.12.3
DOI:
10.4238/2015.May.12.3
Plant Genetics   Research Article

Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica

Authors: H.Y. Yang, X.W. Xia, W. Fang, Y. Fu, M.M. An and M.B. Zhou

Spontaneous leaf color variation in bamboo provides the opportunity to study the mechanisms of leaf color formation and the breeding of ornamental bamboos. Despite the fact that many genes are known to be involved in leaf color variation in model plants, molecular mechanisms governing natural leaf color variation in bamboo hav.. Read More»

Genet. Mol. Res. 14(4):
2015.October.2.16
DOI:
10.4238/2015.October.2.16
Plant Genetics   Research Article

Genome elimination during microsporogenesis in two pentaploid accessions of Brachiaria decumbens (Poaceae)

Authors: G.C.L. Ricci, M.S. Pagliarini and C.B. Valle

Polyploidy is a prominent and significant force in plant evolution, taking place since ancient times and continuing until today. Recent cytogenetic studies in the genus Brachiaria using germplasm collected from wild African savannas in the 1980s revealed that most species and accessions within species are polyploid. Diploid, t.. Read More»

Genet. Mol. Res. 9(4):
vol9-4gmr919
DOI:
10.4238/vol9-4gmr919
Human Genetics   Research Article

Postnatal diagnosis of constitutive ring chromosome 13 using both conventional and molecular cytogenetic approaches

Authors: L.B. Minasi, I.P. Pinto, J.G. de Almeida, A.V. de Melo,D.M.C. Cunha, C.L. Ribeiro, G.P. Silva, M.G. Brasil, D.M. Silva, C.C. da Silva and A.D. da Cruz

We describe the first postnatal diagnosis of a child from Central Brazil with de novo cytogenetic alterations in 13q showing malformations of the brain, eyes, distal limbs, and genitourinary tract, and severe intellectual disability. The karyotype was a constitutive 46,XX,r(13)[77]/45,XX,-13[17]/46,XX,idic r(13)[6]. Interphase and metaphase fluorescence i.. Read More»

Genet. Mol. Res. 14(1):
2015.March.6.15
DOI:
10.4238/2015.March.6.15
Plant Genetics   Research Article

Identification of floral genes for sex determination in Calamus palustris Griff. by using suppression subtractive hybridization

Authors: C.Y. Ng, R. Wickneswari and C.Y. Choong

Calamus palustris Griff. is an economically important dioecious rattan species in Southeast Asia. However, dioecy and onset of flowering at 3-4 years old render uncertainties in desired female:male seedling ratios to establish a productive seed orchard for this rattan species. We constructed a subtractive library for .. Read More»

Genet. Mol. Res. 13(3):
2014.August.7.18
DOI:
10.4238/2014.August.7.18
Plant Genetics   Research Article

Breeding new seedless grape by means of in vitro embryo rescue

Authors: W. Ji, Z.-Q. Li, Q. Zhou, W.-K. Yao and Y.-J. Wang

This project aimed at breeding new seedless grape cultivars by embryo rescue through three hybridization methods: 1) using cross-breeding between seedless Vitis vinifera cultivars and wild Chinese Vitis spp; 2) crossing with two seedless cultivars, and 3) hybridization between grapes of different ploidy. Genotype, sampling times, and media were confirmed .. Read More»

Genet. Mol. Res. 12(1):
2013
DOI:
10.4238/2013
Plant Genetics   Research Article

Microsporogenesis in Brachiaria dictyoneura (Fig. & De Not.) Stapf (Poaceae: Paniceae)

Authors: C. Risso-Pascotto, M.S. Pagliarini and C.B. Valle

Microsporogenesis was analyzed in five accessions of Brachiaria dictyoneura presenting x = 6 as the basic chromosome number. All accessions were tetraploid (2n = 4x = 24) with chromosome pairing in bi-, tri-, and quadrivalents. The recorded meiotic abnormalities were those typical of polyploids, including precocious chromosome.. Read More»

Genet. Mol. Res. 5(4):
Animal Genetics   Research Article

Genetic variability in species of bats revealed by RAPD analysis

Authors: P.R.L. Moreira and E. Morielle-Versute

Random amplified polymorphic DNA molecular marker was utilized as a means of analyzing genetic variability in seven bat species: Molossus molossus, M. rufus, Eumops glaucinus, E. perotis, Myotis nigricans, Eptesicus furinalis, and Artibeus planirostris. The determination of genetic diversity was based on 741 bands produced by .. Read More»

Genet. Mol. Res. 5(4):